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Plasmid#189781PurposeCas12j2 (CasĪ¦) Gateway gRNA entry plasmid using Zea mays Ubi promoter and HH, HDV ribozyme processingDepositorInsertgRNA cloning site
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGlomyc-Ovol1
Plasmid#172871PurposeMammalian expression vector with myc-tagged mouse Ovol1 coding sequenceDepositorInsertOvol1 (Ovol1 Mouse)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
CaV1.3e[8a,11,31b,Ī32,42a]
Plasmid#49333PurposeRepaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].DepositorInsertCACNA1D (Cacna1d Rat)
UseTagsExpressionMammalianMutationContains exons 8a, 11, 31b and 42a.Deletion of exā¦PromoterCMVAvailable sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-MTOR-L1460P
Plasmid#69006Purposeactivating MTOR mutationDepositorInsertMTOR-L1460P (MTOR Human)
UseTagsFLAGExpressionMammalianMutationchange Leucine 1460 to ProlinePromoterAvailable sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ADAR2DD_E488Q
Plasmid#205598Purposeexpresses human ADAR2 deaminase domain (E488Q hyperactive mutant) in mammalian cellsDepositorInsertADAR2 deaminase domain (ADARB1 Human)
UseTagsExpressionMammalianMutationE488Q hyperactive mutationPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLB038 - PHS1āP
Plasmid#136285Purposeentry vector for GreenGate cloning method, C module, open reading frame of phosphatase-inactive PROPYZAMIDE-HYPERSENSITIVE 1 (PHS1) (PHS1DP)DepositorInsertPHS1āP
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cas12f-GE ver4.1
Plasmid#176544PurposeExpresses Cas12f-GE in mammalian cellsDepositorInsertsCas12f-GE ver4.1
Cas12f-GE ver4.1
UseTagsExpressionMammalianMutationPromoterU6 and chicken Ī²-actinAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-MTOR-T1977R
Plasmid#69011Purposeactivating MTOR mutationDepositorInsertMTOR-T1977R (MTOR Human)
UseTagsFLAGExpressionMammalianMutationchange Threonine 1977 to ArgininePromoterAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30a-HS-Tn5
Plasmid#127916PurposeProkaryotic expression of Hyperstable Tn5 (HS-Tn5) for Illumina library preperation. Developed for the large scale ChIP-Seq and ATAC-Seq experiments for the fruitENCODE and Maize Cistrome projects.DepositorInsertHyperstable Tn5
UseTagsE coli elongation factor Ts and Mxe intein - Chitā¦ExpressionBacterialMutationE54K, L372PPromoterT7Available sinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
MYH11-gRNA1
Plasmid#132587PurposegRNA used for knockin NanoLuc and tdTomado (separated by 2A) into MYH11 allele (MYH11-NanoLuc-2A-tdTomato vector) )DepositorInsertMYH11-gRNA1
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ADAR2DD_E488Q_Ago2
Plasmid#205599Purposeexpresses human ADAR2 deaminase domain (E488Q hyperactive mutant) fused with human Ago2 in mammalian cellsDepositorInserthAgo2 (AGO2 Human)
UseTagsADAR2DD_E488Q (hyperactive mutant)ExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD21-hgAPOAI
Plasmid#124962PurposeExpress ApoAI in the liverDepositorInserthuman APOAI gene (LPA Human)
UseAdenoviralTagsExpressionMutationPromoterhuman APOAIAvailable sinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-PGC-1alpha1
Plasmid#45501DepositorInsertPGC-1alpha Isoform1 (Ppargc1a Mouse)
UseTags6xHis and FlagExpressionMammalianMutationQ339K relative to NM_008904PromoterAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pD21-AI-hLDLR
Plasmid#124964PurposeExpress human LDLR under human APOAI promoterDepositorInserthuman LDLR (LDLR Human)
UseAdenoviralTagsExpressionMutationPromoterhuman APOAIAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-GST-4EBP1-Low GC
Plasmid#69018Purposeexpresses tagged 4EBP1DepositorInsertEIF4EBP1 (EIF4EBP1 Human)
UseTagsGST and HAExpressionMammalianMutationlowGC content, wt is ~70% GC rich. Still, it codeā¦PromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2B EDD
Plasmid#37188DepositorInsertEDD (UBR5 Human)
UseTags6x His Tag and FLAGExpressionMammalianMutationPlease see Depositor CommentsPromoterCMVAvailable sinceSept. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2B EDD C2768A
Plasmid#37189DepositorInsertEDD C2768A (UBR5 Human)
UseTags6x His Tag and FLAGExpressionMammalianMutationChanged Cysteine 2768 to AlaninePromoterCMVAvailable sinceJuly 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
Pinducer20-BSD-scFv-sfGFP-DNMT3A1
Plasmid#115780Purposetargeted DNA methylation toolDepositorInsertscFv-sfGFP-DNMT3A1 (DNMT3A Human)
UseTagsExpressionMammalianMutationPromoterTREAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSI2-DCD
Plasmid#166968PurposeExpress the fusion protein of human MSI2 and the catalytic dead domain of Drosophila ADARDepositorInsertMSI2-DCD
UseRetroviralTagsMSI2-DCDExpressionMammalianMutationN495SPromoterMSCVAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-MTOR-I2500F
Plasmid#69014Purposeactivating MTOR mutationDepositorInsertMTOR-I2500F (MTOR Human)
UseTagsFLAGExpressionMammalianMutationchange Isoleucine 2500 to PhenylalaninePromoterAvailable sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only