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Plasmid#67547PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae XI-2 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only
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C-1-BE
Plasmid#135029PurposeC-T base editing using PmCDA1L1_4 fused to C terminal of nCas9DepositorInsertPmCDA1L1_4
UseCRISPRTagsExpressionMammalianMutationPromoterU6 with EF-1alpha intronAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHJ1_Full length LexA
Plasmid#107237PurposesfGFP expression repressed by transcriptional factor LexADepositorInsertFull length LexA repressor
UseSynthetic BiologyTagsExpressionMutationPromoterpLacO1Available sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
SaMscL (CDelta26)
Plasmid#79027PurposeStaphylococcus aureus MscL with C Terminal Truncation of 26 amino acidsDepositorInsertMscL
UseTags6x Histidine TagExpressionMutationStop codon placed before 26 amino acids at C Term…PromoterT7 PromoterAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJLI-sup35
Plasmid#1210DepositorInsertsup35 5' and 3' flanking regions (SUP35 Budding Yeast)
UseTagsExpressionYeastMutationsup35 gene is not included in this plasmid. Only…PromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
N-4-BE
Plasmid#135019PurposeC-T base editing using LjCDA1L1_4 fused to N terminal of nCas9DepositorInsertLjCDA1L1_4
UseCRISPRTagsExpressionMammalianMutationPromoterU6 with EF-1alpha intronAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCaSpeR-act5cB-LexAQFw
Plasmid#61311PurposeExpresses chimeric transactivator LexA_DBD:QF2w_AD in DrosophilaDepositorInsertLexA_DBD::QF2w_AD
UseTagsExpressionInsectMutationChanged EK on C-terminus of QF2 to KKKKPromoterAvailable sinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pattB-synaptobrevin-15-LexABD-G4MD-QFAD-hsp70
Plasmid#46124DepositorInsertLexABD-G4MD-QFAD
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCfB2223
Plasmid#67544PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae X-3 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLei-wt-sfGFP
Plasmid#191113PurposeAn E. coli sfGFP wild type controlDepositorInsertsuperfolder green fluorescence protein
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T5-MfnG-TEV-His
Plasmid#191114PurposeExpresses MfnG-TEV-His in E. coliDepositorInsertMfnG
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC2483 (pSpy1C)
Plasmid#220248Purposereplicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK484 (2mAID-Hygro)
Plasmid#214396Purpose2mAID for N-terminal taggingDepositorInsert2mAID-Hygro
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK485 (3mAID-Hygro)
Plasmid#214397Purpose3mAID for N-terminal taggingDepositorInsert3mAID-Hygro
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK454 (dTAG-BSD)
Plasmid#214384PurposedTAG for C-terminal taggingDepositorInsertdTAG-BSD
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA2.1
Plasmid#208300PurposeExpresses ABE-RA2.1 in mammalian cellsDepositorInsertTadA-RA2.1-SpCas9 D10A
UseTagsExpressionMammalianMutationP48A, R51H, D108G, K110R, H122R, M126I, N127KPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
FB430
Plasmid#203621PurposeTU for the expression of phleomycin resistance.DepositorInsertPpcbC:ble:TamdS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB431
Plasmid#203622PurposeTU for the expression of terbinafine resistance.DepositorInsertPgpdA:ergA:TamdS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBM10a
Plasmid#179688PurposeTarget plasmid for context profilingDepositorInsertACA target
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only