Addgene

We narrowed to 13,670 results for: Coli

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pAW158

Plasmid

#113177

Purpose

E. coli expression vector for untagged E. coli Cas1 R112E + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Use

Tags

Expression

Bacterial

Mutation

R112E

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCK354

Plasmid

#129698

Purpose

For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120010799

Depositor

John Heap

Article

Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m

Inserts

PrhaBAD
yfp
rhaS
Terminator ECK120010799

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCK351

Plasmid

#129696

Purpose

For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120034435

Depositor

John Heap

Article

Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m

Inserts

PrhaBAD
yfp
rhaS
Terminator ECK120034435

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCK353

Plasmid

#129697

Purpose

For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120015170

Depositor

John Heap

Article

Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m

Inserts

PrhaBAD
yfp
rhaS
Terminator ECK120015170

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCK355

Plasmid

#129699

Purpose

For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + ilvBN terminator

Depositor

John Heap

Article

Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m

Inserts

PrhaBAD
yfp
rhaS
ilvBN terminator

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pJK515

Plasmid

#72385

Purpose

Produces Escherichia coli glycinamide ribonucleotide formyltransferase 2 (EcPurT)

Depositor

T. J. Kappock

Insert

phosphoribosylglycinamide formyltransferase 2

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pKPY115

Plasmid

#72669

Purpose

Encodes Thr251Gly-EcPheRS Under Arabinose-Inducible (PBAD) Control

Depositor

David Tirrell

Insert

Thr251Gly-EcPheRS

Use

Tags

Expression

Bacterial

Mutation

Thr251Gly in Alpha Subunit

Promoter

PBAD

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCASCADE-proD

Plasmid

#65820

Purpose

Const. Promoter ProD targetting RNA E. coli Low Phosphate Induction

Depositor

Michael Lynch

Insert

proD guide

Use

Tags

Expression

Mutation

Promoter

E . coli ugpB gene promoter, low phosphate induc…

Available since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

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pHflu2

Plasmid

#203880

Purpose

pHI1 with insertion of ampicillin-resistance gene

Depositor

Bogumil Karas

Insert

broad-spectrum class A beta-lactamase TEM-116

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pHflu3

Plasmid

#203881

Purpose

pHI1 with insertion of kanamycin-resistance gene

Depositor

Bogumil Karas

Insert

aminoglycoside O-phosphotransferase APH(3')-IIa

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pDL17

Plasmid

#106483

Purpose

pDL17 is based on conditionally replicating backbone derived from pQE-30 and expresses gam, bet and exo genes under control of PrhaB promoter for efficient dsDNA integration into E. coli chromosome

Depositor

Sergey Sineoky

Article

Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0

Inserts

gam
bet
exo
lacI
rhaR
rhaS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pSCM001

Plasmid

#124605

Purpose

Expresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pH

Depositor

David Summers

Article

Zarkan et al Sci Rep. 2019 Mar 7;9(1):3868. doi: 10.1

Insert

mCherry-pHluorin

Use

Tags

mCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…

Expression

Bacterial

Mutation

Promoter

pDAB

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD24-rnc-sfGFP

Plasmid

#51560

Purpose

Gene encoding E. coli RNaseIII cloned as a N terminal translational fussion with superfolder GFP. Expression driven by arabinose (backbone is pBAD24).

Depositor

Sankar Adhya, Francisco Malagon

Article

Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

Insert

rnc (rnc E. coli)

Use

Tags

GLESTCRHASLAVLADERRFSA and superfolder GFP

Expression

Bacterial

Mutation

Contains additonal aa at the end of the sfGFP in …

Promoter

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pET24a-AlkB deltaN11

Plasmid

#73622

Purpose

Expresses E. coli AlkB absent the first 11 amino acids and with a C-terminal histidine tag

Depositor

Catherine Drennan

Article

Frick et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

AlkB (alkB E. coli)

Use

Tags

His

Expression

Bacterial

Mutation

deletion of first 11 AA

Promoter

T7

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only
EcMscL

Plasmid

#92418

Purpose

E. coli Mechanosensitive Channel of Large Conductance

Depositor

Douglas Rees

Insert

MscL (mscL E. coli)

Use

Tags

6x Histidine Tag and Thrombin Cleavage Site betwe…

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pJK649

Plasmid

#72399

Purpose

Produces Escherichia coli phosphoribosylpyrophosphate amidotransferase (EcPurF)

Depositor

T. J. Kappock

Insert

amidophosphoribosyltransferase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pCASCADE-zwf

Plasmid

#65825

Purpose

zwf targeting guide RNA E. coli , Low Phosphate Induction

Depositor

Michael Lynch

Insert

zwf guide

Use

Tags

Expression

Bacterial

Mutation

Promoter

E . coli ugpB gene promoter, low phosphate induc…

Available since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pCASCADE-udhA

Plasmid

#65818

Purpose

udhA targeting guide RNA E. coli , Low Phosphate Induction

Depositor

Michael Lynch

Insert

udhA guide

Use

Tags

Expression

Bacterial

Mutation

Promoter

E . coli ugpB gene promoter, low phosphate induc…

Available since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pCASCADE-gltA2

Plasmid

#65817

Purpose

gltA targeting guide RNA E. coli , Low Phosphate Induction

Depositor

Michael Lynch

Insert

gltA2 guide

Use

Tags

Expression

Bacterial

Mutation

Promoter

E . coli ugpB gene promoter, low phosphate induc…

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pHL1914

Plasmid

#53042

Purpose

PconNoHindM2:hfqlead::gfp, PconNoHind:RBS(st3):tetR

Depositor

Han Lim

Inserts

GFP
tetR

Use

Synthetic Biology

Tags

RBS(st3) and hfqlead

Expression

Bacterial

Mutation

Promoter

PconNoHindM2 and pLlacO-1

Available since

May 13, 2014

Availability

Academic Institutions and Nonprofits only