Addgene

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KHT-1

Plasmid

#113054

Purpose

Expresses worm KHT-1 in mammalian cells

Depositor

Federico Sesti

Article

Cai et al EMBO J. 2009 Jun 3;28(11):1601-11. doi:

Insert

K+ channel for Habituation to Tap subunit 1

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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GAL-MAGE-2-429

Plasmid

#89126

Purpose

mammalian expression of full-length human MAGE-A11 with N-terminal GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

Insert

MAGE-A11 (MAGEA11 Human)

Use

Tags

GAL4 DBD

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only
VP-MAGE-2-429

Plasmid

#89125

Purpose

mammalian expression of full-length human MAGE-A11 with N-terminal VP16 activation domain

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

Insert

MAGE-A11 (MAGEA11 Human)

Use

Tags

VP16

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only
pGEX2TK E7 del EDE

Plasmid

#13675

Depositor

Karl Munger

Article

Jones et al Genes Dev. 1997 Aug 15. 11(16):2101-11.

Insert

HPV 16 E7 del EDE (E7 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

del aa 35-37

Promoter

Available since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
pUAST tdTomato Rab11 WT

Plasmid

#53473

Depositor

Matthew Scott

Insert

Rab11 (Rab11 Fly)

Use

Tags

tdtomato

Expression

Insect

Mutation

Promoter

GAL4

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
pTIN

Plasmid

#162885

Purpose

Overexpression vector for the fission yeast Schizosaccharomyces pombe

Depositor

Stewart Shuman

Article

Garg et al RNA. 2020 Nov;26(11):1743-1752. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMemoryArray

Plasmid

#60585

Purpose

An array constructed by concatenating the attB/P sites of the 11 phage integrases and can be used to distinguish 2048 possible combinations of events.

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

MemoryArray

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-TREX1(D18N)

Plasmid

#27220

Depositor

Judy Lieberman

Article

Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

Insert

TREX1 (TREX1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

D18N sequence 52-GAC changed to AAC, resulting i…

Promoter

Available since

Jan. 26, 2011

Availability

Academic Institutions and Nonprofits only

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pSopB2xHA

Plasmid

#112513

Purpose

PsopB driven sopB-2xHA and sigE

Depositor

Olivia Steele-Mortimer

Article

Knodler et al Cell Microbiol. 2009 Nov;11(11):1652-70.

Insert

sopB-2xHA/sigE operon

Use

Tags

2xHA

Expression

Bacterial

Mutation

SopB C-terminal 2xHA

Promoter

PsopB

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pSG5-MAGE-TA360

Plasmid

#89141

Purpose

mammalian expression of full-length human MAGE-A11 amino acid residues 2-429 with TA360 phosphorylation site mutated

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2008 Mar;28(6):1947-63. d

Insert

MAGE-A11 (MAGEA11 Human)

Use

Tags

Expression

Mammalian

Mutation

T360A

Promoter

SV40

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only
Anti-Jagged [D5] (Recombinant Antibody)

Antibody

#201864-rAb

Purpose

Anti-Jagged (Human) recombinant scFv-Fc-fusion

Depositor

John McCafferty

Article

Schofield et al Genome Biol. 2007;8(11):R254.

Recommended Applications

Immunocytochemistry

Reactivity

Human

Source Species

Human

Isotype

IgG1

Trial Size

Available to purchase

Available since

Nov. 8, 2023

Availability

Industry, Academic Institutions, and Nonprofits
pTIN7m

Plasmid

#162886

Purpose

Overexpression vector for the fission yeast Schizosaccharomyces pombe (lower strength)

Depositor

Stewart Shuman

Article

Garg et al RNA. 2020 Nov;26(11):1743-1752. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pSG5HA-MAGE-KA240,245

Plasmid

#89140

Purpose

mammalian expression of N-terminal HA-tagged full-length human MAGE-A11 amino acid residues 2-429 with KA240,KA245 ubiquitination sites mutated

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2008 Mar;28(6):1947-63. d

Insert

MAGE-A11 (MAGEA11 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K240A, K245A

Promoter

SV40

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pSG5HA-MAGE-112-429

Plasmid

#89131

Purpose

mammalian expression of N-terminal HA-tagged human MAGE-A11 amino acid residues 112-429

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2008 Mar;28(6):1947-63. d

Insert

MAGE-A11 (MAGEA11 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

contains aa 112-429

Promoter

SV40

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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C321.∆A T7RNAP

Bacterial Strain

#182776

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted

Depositor

Michael Jewett

Article

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

Bacterial Resistance

Spectinomycin

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only
Anti-Kir6.2 K+ channel [N363/71R] (Recombinant Antibody)

Antibody

#209595-rAb

Purpose

Anti-Kir6.2 K+ channel (Rat) recombinant mouse monoclonal antibody

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Recommended Applications

Immunocytochemistry

Reactivity

Mouse and Rat

Source Species

Mouse

Isotype

IgG2a

Trial Size

Available to purchase

Available since

Nov. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits
Anti-Desmin [D7] (Recombinant Antibody)

Antibody

#201865-rAb

Purpose

Anti-Desmin (Human) recombinant scFv-Fc-fusion

Depositor

John McCafferty

Article

Schofield et al Genome Biol. 2007;8(11):R254.

Recommended Applications

ELISA

Reactivity

Human

Source Species

Human

Isotype

IgG1

Trial Size

Available to purchase

Available since

Oct. 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits
HSD11B1

Plasmid

#39155

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

HSD11B1 (HSD11B1 Human)

Use

Tags

His Tev

Expression

Bacterial

Mutation

T220I; codon-optimized

Promoter

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-C3-hYAP1

Plasmid

#17843

Depositor

Marius Sudol

Article

Basu et al Mol Cell. 2003 Jan . 11(1):11-23.

Insert

YAP (YAP1 Human)

Use

Tags

EGFP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 29, 2008

Availability

Academic Institutions and Nonprofits only

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MSCV Myc T58A puro

Plasmid

#18773

Depositor

Scott Lowe

Article

Hemann et al Nature. 2005 Aug 11. 436(7052):807-11.

Insert

myc T58A (MYC Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

T58A

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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