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pSG5-Myr-FLAG-p110β-DM

Plasmid

#55719

Purpose

eukaryotic expression of myristoylation tag (Myr-FLAG) p110b RBD double mutant S205D, K224A

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

p110 beta

Use

Tags

myristoylation tag (Myr-FLAG)

Expression

Mammalian

Mutation

S205D, K224A

Promoter

T7

Available since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pYZ_NAND2_L17_S19_S11

Plasmid

#132730

Purpose

T7 RNAP-driven expression of a 2-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 19 and 11

Depositor

Peng Yin, Alexander Green

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_NAND2_L17_S19_S11

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pNMHCII-C1C2-GFP

Plasmid

#46044

Depositor

Robert Adelstein

Article

Jana et al J Biol Chem. 2009 Apr 24;284(17):11563-7

Insert

nonmuscle myosin heavy chain II-C, isoform C1C2 (Myh14 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV immediate early

Available since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only
pMDC84-SEIPIN3-Ndelta

Plasmid

#96981

Purpose

Express N-terminal truncated Arabidopsis SEIPIN3 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN3 (AT2G34380 Mustard Weed)

Use

Tags

6xHis and GFP

Expression

Plant

Mutation

A segment of 507 nucleotides are deleted from the…

Promoter

35S

Available since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(RBSmut)_Psyn-sgRNArodA

Plasmid

#149656

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30, Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe-SIRT3

Plasmid

#66167

Purpose

Expresses SIRT3 via retroviral method

Depositor

Ronald Kahn

Article

Jing et al Diabetes. 2013 Oct;62(10):3404-17. doi:

Insert

SIRT3 (Sirt3 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

L235P

Promoter

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only
psbA2(noATbox)-PHLS (c)

Plasmid

#52308

Purpose

Replaces the psbA2 gene in Synechocystis with the PHLS gene

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2014 Aug;240(2):309-24. doi: 10.

Inserts

beta-phellandrene synthase
Chloramphenicol resistance

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

CAAATACA box in the psbA2 promoter to ggcgcgcc

Promoter

psbA2

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA3 humanN-Flag SadBS

Plasmid

#66881

Purpose

Mammalian expression vector for expressing N-terminally Flag-tagged short isoform of SadB

Depositor

Maria Alvarado-Kristensson

Article

Eklund et al J Biol Chem. 2014 Aug 1;289(31):21360-73

Insert

SadB (BRSK1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
cpc-PHLS (g)

Plasmid

#52311

Purpose

Replaces the cpc operon in Synechocystis with the PHLS gene

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2014 Aug;240(2):309-24. doi: 10.

Inserts

beta-phellandrene synthase
Chloramphenicol resistance

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Synechocystis endogenous cpc-operon promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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Dll3ICD-HPC4 pBacPAK8-3

Plasmid

#17334

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Delta-like 3 (Dll3 Mouse)

Use

Tags

HPC4

Expression

Insect

Mutation

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pCMV-myc-mIFITM3

Plasmid

#58458

Purpose

Expresses murine IFITM3 with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only
pTPTP alpha (S204A)

Plasmid

#17694

Depositor

Xinmin Zheng

Article

Zheng et al J Biol Chem. 2002 Jun 14. 277(24):21922-

Insert

PTPalpha S204A (PTPRA Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S204A

Promoter

Available since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
pTH734-CEN-minHIS3

Plasmid

#38227

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

HIS3 (HIS3 Synthetic, Budding Yeast)

Use

Tags

HA

Expression

Yeast

Mutation

Every codon of the coding sequence has been repla…

Promoter

TDH3

Available since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pTH737-CEN-maxHIS3

Plasmid

#38229

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

HIS3 (HIS3 Synthetic, Budding Yeast)

Use

Tags

HA

Expression

Yeast

Mutation

Every codon of the coding sequence has been repla…

Promoter

TDH3 (=GPD)

Available since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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GST-AR-4-52

Plasmid

#104199

Purpose

Expresses human AR-4-52 in in pGEX-4T-1 as GST fusion protein

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

Insert

human androgen receptor aa 4-52 (AR Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS_AKAR5’

Plasmid

#114141

Purpose

Mammalian expression of the AKAR5’ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

AKAR5'

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Bmf (S74D)

Plasmid

#24259

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bmf (S87D) (BMF Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed Serine 74 to Aspartic Acid

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag Bmf (S74A)

Plasmid

#24258

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bmf (S87A) (BMF Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed Serine 74 to Alanine

Promoter

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF3H02

Plasmid

#70956

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Iswi (Iswi Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
ATF4 19: (SL)RLuc.5’ATF4.GFP

Plasmid

#21865

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Stem Loop upstream of ATF4

Promoter

Available since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only