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Plasmid#61380PurposeExpresses Human full length RIPK1with I539P mutation in the mammalian cellsDepositorInsertFull length human RIP1with I539P mutation (RIPK1 Human)
UseTagsEGFP and HA tagExpressionMammalianMutationchanged I539 to PPromoterAvailable sinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-TRAF4(K190R)
Plasmid#58319PurposeEGFP-tagged TRAF4 K190R mutantDepositorInsertTNF receptor associated factor 4 (Traf4 Mouse)
UseTagsGFPExpressionMammalianMutationK 190 to RPromoterCMVAvailable sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQuantEGFP-bsr-kana
Plasmid#45590DepositorTypeEmpty backboneUseCre/Lox; Dt40 cell line targetingTagsQuant-EGFP tagExpressionMutationPromoterAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQuantEGFP-puro-kana
Plasmid#45589DepositorTypeEmpty backboneUseCre/Lox; Dt40 cell line targetingTagsQuant-EGFP tagExpressionMutationPromoterAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp4-a W118S
Plasmid#40042DepositorInserthSlp4-a W118S (SYTL4 Human)
UseTagsEGFPExpressionMammalianMutationTryptophane 118 to SerinePromoterCMV promoterAvailable sinceOct. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp4-a V21A
Plasmid#40041DepositorInserthSlp4-a V21A (SYTL4 Human)
UseTagsEGFPExpressionMammalianMutationValine 21 to AlaninePromoterCMV promoterAvailable sinceOct. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp4-a SHD
Plasmid#40034DepositorInserthSlp4-a SHD (SYTL4 Human)
UseTagsEGFPExpressionMammalianMutationSHD domain (1-143)PromoterCMV promoterAvailable sinceOct. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-STAG2-D299A
Plasmid#31975DepositorInserthuman STAG2 cDNA (STAG2 Human)
UseTagsEGFP and FLAGExpressionMammalianMutationchanged Aspartic acid 299 to AlaninePromoterAvailable sinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAG304GAL-EGFP-ccdB
Plasmid#14303DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCS2-EcYRS-EGFP*
Plasmid#191118PurposeA plasmid for incorporation of O-Me-Tyr into an EGFP reporter for zebrafishDepositorInsertMutant E. coli TyrRS - T2A - enhanced green fluorescence protein
UseTagsHis tagExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EGFP-SKL
Plasmid#183727PurposeFor exrpression of GFP targeted to peroxisomesDepositorInsertEGFP
UseLentiviralTagsSKL (peroxisomal targeting sequence 1)ExpressionMutationPromoterUbiCAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
MCP-EGFP-PiggyBac
Plasmid#190003PurposeExpression of MCP-EGFP in mammalian cells. To clone this plasmid, Addgene plasmid #119908 was used. We replaced the tandem coat proteins and double tagRFP with a single coat protein fused to EGFP.DepositorInsertMCP-EGFP
UseTagsExpressionMutationPromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EGFP-nonstop
Plasmid#226406PurposeExpresses EGFP without a stop codon that produces a GFP-tagged readthrough RQC substrate.DepositorInsertEGFP without stop codon
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA2-5.0dand5:EGFP
Plasmid#223327PurposeExpresses EGFP in Kupffer's vesicle cells in zebrafish embryosDepositorInsert5.0kb dand5 promoter (dand5 Zebrafish)
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUt-MONARCH 1.0_crEGFP
Plasmid#224790PurposeLPUtopia matching RMCE donor plasmid with MONARCH 1.0 circuit with crRNA targeting EGFP. Use BlastR for positive and HSV-TK for negative selectionDepositorInsertcrEGFP
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMV-d2iAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUt-MONARCH 2.0_crEGFP
Plasmid#224793PurposeLPUtopia matching RMCE donor plasmid with MONARCH 2.0 circuit with crRNA targeting EGFP.DepositorInsertscrEGFP
Triplex stablier
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMV-d2iAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL.mEGFP-CBX8
Plasmid#224710PurposeExpresses N-terminal hexahistidine-tagged human CBX8 in insect cells, with a monomeric variant EGFP tag.DepositorInsertCBX8 (CBX8 Human)
UseTagsmEGFPExpressionInsectMutationPromoterPolyhedrinAvailable sinceOct. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-PERK(LD)-EGFP
Plasmid#225680PurposeUsed to generate stable cell lines that express PERK(LD)-EGFP. This plasmid serves as a control for pLVX-PERK(LD)-EGFP-HOTag3.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-EGFP-GAPDH-CC
Plasmid#225476PurposeExpress mEGFP-GAPDH-CC fusionDepositorInsertGAPDH fused with CC2 domain of ROCK2 (GAPDH Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-UGCG-EGFP
Plasmid#225878PurposePlasmid donor template for CRISPR/Cas9 mediated UGCG knockout by EGFP insertionDepositorInsertEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only