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Plasmid#111269Purposeexpress murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form (Syk Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-TYK2V362F-VSV
Plasmid#139345PurposeExpression of TYK2 V362F protein (rs2304256)DepositorInsertTYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
UseTagsVSVExpressionMammalianMutationV362FPromoterCMVAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBABE-hygro NM23 H1 WT
Plasmid#11364DepositorInsertNM23 H1 (NME1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSico-shRheb
Plasmid#81087Purposeconditional expression of an shRNA targeting murine RhebDepositorInsertRheb shRNA (Rheb Mouse)
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
ATF4 14: 5’ATF4.uORF1&2AUA.GFP
Plasmid#21861DepositorInsertATF4 (Atf4 Mouse)
UseTagsEGFPExpressionMammalianMutationAUG codons of uORFs1&2 were converted to AUAPromoterAvailable sinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBSSK p85 alpha (delta SH3)
Plasmid#13430DepositorInsertp85 alpha (delta SH3) (PIK3R1 Human)
UseTagsFLAGExpressionBacterialMutationSH3 domain replaced by flag tag.PromoterAvailable sinceNov. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_ManII-SBP-tagBFP
Plasmid#65254Purposesynchronize trafficking of ManII from the ER (RUSH system)DepositorInsertStreptavidin-KDEL and truncated Mannosidase II
UseTagsStreptavidin Binding Protein (SBP) and tagBFPExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.1-Pct5.1-crRNA(dadR)
Plasmid#191634PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer)DepositorInsertCymR
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(RBSmut)_Psyn-sgRNAftsI
Plasmid#149590Purposeall-in-one CRISPRi vector for targeting B. burgdorferi ftsIDepositorInsertdCas9, lacI, sgRNAftsI
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSilencer2.1-U6-NL8F100
Plasmid#190852PurposeFor mammalian expression of NL8F100, a dual-specificity aptamer that binds OGT and β-catenin. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker.DepositorInsertNL8F100 (A Dual-Specificity aptamer targeting OGT and β-catenin)
UseAffinity Reagent/ Antibody and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSilencer2.1-U6-3JB1R+6
Plasmid#190842PurposeFor mammalian expression of 3JB1R+6, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.DepositorInsert3JB1R_AP3+6bp (A Dual-Specificity aptamer targeting OGT and GFP)
UseAffinity Reagent/ Antibody and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUAST-attB-Flag-dTSC2 WT
Plasmid#111807PurposeInsect Expression of dTSC2DepositorInsertdTSC2 (gig Fly)
UseTags3xFlagExpressionInsectMutationPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJet-CMV-CD5HA2-bglpA
Plasmid#96962PurposeExpression plasmid for HA-tag embedded into human CD52 with short polyADepositorInsertCMV-CD5HA2-bglpA
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
2Bc-T MBP_BoMoC(ed)_I770A_6xH
Plasmid#185711PurposeRecombinant B. mori truncated R2 RT expressionDepositorInsertB. mori truncated R2 RT
UseTags6xHis and MBPExpressionBacterialMutationChanged I770 to APromoterT7Available sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBSSK p85 alpha (delta BH)
Plasmid#13431DepositorInsertp85 alpha (delta BH) (PIK3R1 Human)
UseTagsFLAGExpressionBacterialMutationBH domain replaced by flag tag.PromoterAvailable sinceNov. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pJZC625
Plasmid#62321PurposesgRNA with 1x MS2, Pol II promoter with ribozyme-gRNA-ribozyme design for yeast cellsDepositorInsertsgRNA +1x MS2, Pol II promoter with ribozyme cleavage
UseTagsExpressionYeastMutationPromoterpAdhAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 Flag Jnk1a1(APY)
Plasmid#15742DepositorInsertJnk1a1(TPF) (MAPK8 Human)
UseTagsFlagExpressionMammalianMutationThr-190AlaPromoterAvailable sinceMarch 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral CaMKKbeta K193A
Plasmid#33323DepositorInsertCalcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
UseLentiviralTagsFlagExpressionMutationK194APromoterhuman PGKAvailable sinceApril 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Fyn C3,6S
Plasmid#19979DepositorInsertFyn (FYN Human)
UseTagsExpressionMammalianMutationC3,6SPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 Flag Jnk1a1(TPF)
Plasmid#15741DepositorInsertJnk1a1(APY) (MAPK8 Human)
UseTagsFlagExpressionMammalianMutationTyr-192PhePromoterAvailable sinceMarch 6, 2008AvailabilityAcademic Institutions and Nonprofits only