-
Plasmid#121729PurposepMVP L5-L4 entry plasmid, contains TRE promoter for 4-component MultiSite Gateway Pro assemblyDepositorInsertTRE promoter
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNL1 Ecarin
Plasmid#106289PurposeExpresses Ecarin in Ecoli off a T7 promoter in the NEB control cloning vectorDepositorInsertEcarin
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C3106
Plasmid#91101PurposeModule C, Promoter: FMV 34S, Gene: TALE-VPR backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSPDepositorInsertTALE-VPR backbone with Esp3I ccdb cassette for repeat cloning
UseTALENTagsExpressionMutationPromoterFMV 34SAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pME-BTK-PH
Plasmid#67673PurposeGateway entry clone containing BTK-PH with no stop codonDepositorInsertBTK-PH-NS
UseGateway multisite middle entry cloneTagsExpressionMutationPromoterAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C4300
Plasmid#91103PurposeModule C, Promoter: none, Gene: BaeI LacZ cassette for GT donor cloning, Terminator: noneDepositorInsertBaeI LacZ cassette for GT donor cloning
UseUnspecifiedTagsExpressionMutationPromoternoneAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
LB801: pMVP/PB/eCFP-DEST
Plasmid#121872PurposepMVP destination vector, empty PiggyBac transposon vector backbone w/ eCFP selection cassetteDepositorTypeEmpty backboneUsePiggybac transposon, pmvp gateway destination vec…TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KU701: pMVP (L5-L4) eGFP
Plasmid#121707PurposepMVP L5-L4 entry plasmid, contains eGFP for 4-component MultiSite Gateway Pro assembly. Allows N-term eGFP fusion to gene of interest.DepositorInserteGFP (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IO901: pMVP (L5-L4) BioID2
Plasmid#121724PurposepMVP L5-L4 entry plasmid, contains BioID2 for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term BioID2 to gene of interest.DepositorInsertBioID2 (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 GABARAPL1 G116
Plasmid#123085PurposeGateway entry clone encoding human GABARAPL1 G116DepositorInsertGamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
UseGateway entry vector / entry cloneTagsExpressionMutationDeleted amino acid 117. Stop codon after G116PromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C3306
Plasmid#91102PurposeModule C, Promoter: FMV 34S, Gene: TALE-VP64 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSPDepositorInsertTALE-VP64 backbone with Esp3I ccdb cassette for repeat cloning
UseTALENTagsExpressionMutationPromoterFMV 34SAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
IG703: pMVP (L1-R5) TRE promoter
Plasmid#121697PurposepMVP L1-R5 entry plasmid, contains TRE promoter for 4-component MultiSite Gateway Pro assemblyDepositorInsertTRE promoter
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pANY1
Plasmid#110949PurposeUniversal mini-vector for efficient cloning of DNA and expression of protein in E. coli.DepositorInsertccd B cassette
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCVL TAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers
Plasmid#50421PurposepTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.DepositorTypeEmpty backboneUseLentiviral and TALENTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMay 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCVL TAL(Nd154C63) SFFV Xba-Sal linker
Plasmid#50420PurposepTAL plasmid compatible with the Golden Gate TALEN cloning system. For cloning of megaTALs or other TAL-fusions and expression in mammalian cells. Meganuclease/fusion is cloned between Xba-Sal sites.DepositorTypeEmpty backboneUseLentiviral and TALENTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMay 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1010
Plasmid#91030PurposeModule A, Promoter: ZmUbi, Gene: TALEN_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALEN_2)DepositorInsertTALEN_1 backbone with Esp3I ccdb cassette for repeat cloning
UseTALENTagsExpressionMutationPromoterZmUbiAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1002
Plasmid#91029PurposeModule A, Promoter: AtUbi10, Gene: TALEN_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALEN_2)DepositorInsertTALEN_1 backbone with Esp3I ccdb cassette for repeat cloning
UseTALENTagsExpressionMutationPromoterAtUbi10Available sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTP-PCR C1
Plasmid#189618PurposeCloned Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteriaDepositorInsertMitochondrial Genome of Thalassiosira pseudonana
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationMinor additional mutations described in manuscriptPromoterAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTP-PCR C4
Plasmid#189621PurposeCloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteriaDepositorInsertReduced Mitochondrial Genome of Thalassiosira pseudonana
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationRepeat region removed, and minor additional mutat…PromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTP-PCR C2
Plasmid#189619PurposeCloned Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteriaDepositorInsertMitochondrial Genome of Thalassiosira pseudonana
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationMinor additional mutations described in manuscriptPromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_R4187C
Plasmid#187141PurposeEntry cloneDepositorInsertHUWE1 (HUWE1 Human)
UseEntry cloneTagsExpressionMutationR4187CPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only