Addgene

We narrowed to 5,016 results for: hwa

Showing: 2701 - 2720 of 5016 results

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pCarCBAE

Plasmid

#117755

Purpose

Codon optimized CarCBAE enzymes from Pectobacterium carotovorum in pBbaA5k backbone

Depositor

Greg Bokinsky

Article

Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

Insert

Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA), Ferredoxin CarE

Use

Tags

Expression

Bacterial

Mutation

Promoter

PLac(UV5)

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLJC6-Flag CASTOR1 I280A

Plasmid

#84497

Purpose

Lentiviral expression of CASTOR2 I280A mutant

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR1 I280A (GATSL3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

I280A

Promoter

sestrin2

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only
XE223 human myc-Chibby-pCS2+MT

Plasmid

#16825

Depositor

Randall Moon

Article

Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

Insert

myc-Chibby (CBY1 Human)

Use

Xenopus expression

Tags

6-myc

Expression

Mutation

Promoter

Available since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pMXs-EGFP-ERas delCAAX-IP

Plasmid

#13834

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

ERas delCAAX (Eras Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Deletion 4 amino acids of c-terminal end (CAAX mo…

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pMXs-EGFP-ERas C220S/C222S-IP

Plasmid

#13829

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

ERas C220S/C222S (Eras Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

C220 and C222 changed to serine residues

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
GST-CHMP4A (1-204) (pGEX2T(TEV))

Plasmid

#80632

Purpose

Residues 1-204 of CHMP4A; Bacterial Expression vector; TEV-cleavable GST tag; Sundquist Lab internal ID: WISP06-198

Depositor

Wesley Sundquist

Article

McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

Insert

CHMP4A (CHMP4A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Residues 1-204

Promoter

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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RF-211-UB-Lyn

Plasmid

#79184

Purpose

Expression of the C-terminal fragment of Lyn kinase that is endogenously generated by caspase cleavage in mammalian cells

Depositor

Richard Fahlman

Article

Eldeeb et al Oncotarget. 2014 May 15;5(9):2714-22.

Insert

Lyn (LYN Human)

Use

Tags

3x Flag and ubiquitin

Expression

Mammalian

Mutation

delta 1-18

Promoter

CMV

Available since

June 20, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-EGFP-ERas SSVA-IP

Plasmid

#13827

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

ERas SSVA (Eras Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

C224 changed to serine

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
EPHB4-gRNA1/2

Plasmid

#139974

Purpose

gRNA used for knockin EGFP into EPHB4 allele

Depositor

James Thomson

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jul 25;11

Insert

EPHB4 gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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EFNB2-gRNA3/4

Plasmid

#139973

Purpose

gRNA used for knockin tdTomado into EFNB2 allele

Depositor

James Thomson

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jul 25;11

Insert

EFNB2-gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pZE21-APX(W41F) dimeric

Plasmid

#58199

Purpose

Bacterial expression of APX W41F under control of TetR

Depositor

Alice Ting

Article

Dwyer et al Proc Natl Acad Sci U S A. 2014 May 20;11

Insert

pea APX W41F

Use

Tags

Expression

Bacterial

Mutation

W41F

Promoter

pL(tetO-1)

Available since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-YAP-S381A

Plasmid

#33090

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 )

Use

Tags

Flag

Expression

Mammalian

Mutation

S381A

Promoter

CMV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pENTR-TER+-shLKB1-Ms

Plasmid

#61234

Purpose

Entry clone encoding shRNA to mouse LKB1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

shLkb1 (Ms/Hu) (STK11, Stk11 Mouse, Human)

Use

Entr clone

Tags

Expression

Mutation

Promoter

H1/TO

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-hSOX10-Val15AlafsX11

Plasmid

#24732

Depositor

William Pavan

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

(sex determining region Y)-box 10 (SOX10 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

deleted amino acids 44-62. deletion/frameshift…

Promoter

Available since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3 merlin 308-579

Plasmid

#11626

Depositor

Vijaya Ramesh

Article

Xu et al Exp Cell Res. 1998 Jan 10. 238(1):231-40

Insert

merlin (NF2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Carboxy-terminal common domain of merlin.

Promoter

Available since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
pRK5-HA CASTOR2 ACT2

Plasmid

#84494

Purpose

overexpression of CASTOR2 ACT2 domain (aa 171-end)

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR2 ACT2 (GATSL2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

171-last amino acid

Promoter

CMV

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-Flag CASTOR2 ACT1

Plasmid

#84493

Purpose

overexpression of CASTOR2 ACT1 domain (aa 1-171)

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR2 ACT1 (GATSL2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

1-171 amino acid

Promoter

CMV

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-HA CASTOR1 ACT2

Plasmid

#84492

Purpose

overexpression of CASTOR1 ACT2 domain (aa 169-end)

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR1 ACT2 (GATSL3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

169-last amino acid

Promoter

CMV

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-Flag CASTOR1 ACT1

Plasmid

#84491

Purpose

overexpression of CASTOR1 ACT1 domain (aa 1-169)

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR1 ACT1 (GATSL3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

1-169 amino acid

Promoter

CMV

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-ERas CVLS-IP

Plasmid

#13828

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

ERas CVLS (Eras Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

CSVA (224-227) changed to CVLS (CAAX sequence of …

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only