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Addgene
Showing: 2701 - 2720 of 4629 results
  1. mFzd4CRDL(42-203)-6xHis_pAcGP67a

    Plasmid
    #216379
    Purpose
    Baculovirus transfer vector to secrete Fzd4 cysteine-rich domain and linker (residues 42-203, codon-optimized for insect cell expression, with a C-terminal His tag) in insect cells
    Depositor
    Insert
    Frizzled-4 (Fzd4 Mouse)
    Use
    Baculovirus
    Tags
    6xHis and GP64 signal sequence
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin
    Availability
    Academic Institutions and Nonprofits only
  2. AAV pEF1a-DIO-FLPo-WPRE-hGHpA (AAV Retrograde)

    Viral Prep
    #87306-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from AAV pEF1a-DIO-FLPo-WPRE-hGHpA (#87306). In addition to the viral particles, you will also receive purified AAV pEF1a-DIO-FLPo-WPRE-hGHpA plasmid DNA. EF1a-driven, Cre dependent expression of the site-specific recombinase FlpO. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Promoter
    EF1a
    Tags
    None
    Availability
    Academic Institutions and Nonprofits only
  3. pTR-UF11 (AAV.A08.T)

    Viral Prep
    #157970-AAV.A08.T
    Purpose
    Ready-to-use AAV2(4pMut)dHS in BSS particles produced from pTR-UF11 (#157970). In addition to the viral particles, you will also receive purified pTR-UF11 plasmid DNA. 'Humanized' GFP driven by Chimeric CMV/Chicken Beta Actin promoter
    Depositor
    Promoter
    chimeric CMV/Chicken Beta actin (CBA)
    Tags
    GFP
    Availability
    Academic Institutions and Nonprofits only
  4. pTR-UF11 (AAV.A10.T)

    Viral Prep
    #157970-AAV.A10.T
    Purpose
    Ready-to-use AAV6(dbY-F+T-V) in TMN200 particles produced from pTR-UF11 (#157970). In addition to the viral particles, you will also receive purified pTR-UF11 plasmid DNA. 'Humanized' GFP driven by Chimeric CMV/Chicken Beta Actin promoter
    Depositor
    Promoter
    chimeric CMV/Chicken Beta actin (CBA)
    Tags
    GFP
    Availability
    Academic Institutions and Nonprofits only
  5. RABEP1A-c019

    Plasmid
    #175477
    Purpose
    Protein expression in insect cells. Entire CDS, M1-T862. Codon optimised.
    Depositor
    Insert
    RABEP1 (RABEP1 Human)
    Use
    Tags
    TEV-TwinStrep
    Expression
    Insect
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  6. RABGEF1A-c014

    Plasmid
    #175478
    Purpose
    Protein expression in insect cells. Entire CDS, M1-G491. Codon optimised.
    Depositor
    Insert
    RABGEF1 (RABGEF1 Human)
    Use
    Tags
    His6-Zbasic-TEV
    Expression
    Insect
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  7. SYKA-c014

    Plasmid
    #175488
    Purpose
    Protein expression in insect cells. Entire CDS, M1-N635. Codon optimised.
    Depositor
    Insert
    SYK (SYK Human)
    Use
    Tags
    His6-Zbasic-TEV
    Expression
    Insect
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  8. pET32bplus_trx_dAChE4

    Plasmid
    #83917
    Purpose
    Encodes an E. coli codon optimized version of dAChE4 - a designed variant of human acetylcholinesterase bearing 51 mutations with ~1800 fold higher functional expression levels in E. coli
    Depositor
    Insert
    Designed hAChE, codon optimised for E.coli expression (ACHE Synthetic, Human)
    Use
    Tags
    His, S-tag, and TRX
    Expression
    Bacterial
    Mutation
    V13T,K24T,M43V,L49R,V61W,S68N,E92N,T110K,S111N,T1…
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
  9. pDONR223 SARS-CoV-2 NSP12_nostop

    Plasmid
    #149314
    Purpose
    Gateway-compatible Entry vector, with insert of NSP12 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
    Depositor
    Insert
    NSP12 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
  10. pDONR223 SARS-CoV-2 NSP13_nostop

    Plasmid
    #149315
    Purpose
    Gateway-compatible Entry vector, with insert of NSP13 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
    Depositor
    Insert
    NSP13 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
  11. pBUE411

    Plasmid
    #62200
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
    Depositor
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    OsU3 and Ubi
    Availability
    Academic Institutions and Nonprofits only
  12. pHSE401

    Plasmid
    #62201
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    35S and AtU6-26p
    Availability
    Academic Institutions and Nonprofits only
  13. pHUE411

    Plasmid
    #62203
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Hyg resistance
    Depositor
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    OsU3 and Ubi
    Availability
    Academic Institutions and Nonprofits only
Showing: 2701 - 2720 of 4629 results