Addgene

We narrowed to 4,391 results for: lum

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pcDNA5-Flag-Med25

Plasmid

#64771

Purpose

Mammalian protein expression of flag-tagged Med25

Depositor

Ronald Conaway, Joan Conaway

Article

Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

Insert

Med25 (MED25 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
Flag-Bcl2-Maob

Plasmid

#18005

Depositor

Clark Distelhorst

Article

Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

Insert

Mitochondria-targeted Bcl-2 (BCL2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

C terminal of Bcl-2 replaced with Maob. Targets B…

Promoter

CMV

Available since

June 4, 2008

Availability

Academic Institutions and Nonprofits only
Flag_HsIRE1a_deltaP29_D408_pBabePuro

Plasmid

#58422

Purpose

retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408

Depositor

David Ron

Article

Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

Insert

IRE1a (ERN1 Human)

Use

Retroviral

Tags

FLAG and preprotrypsin signal sequence

Expression

Mammalian

Mutation

Deleted amino acids P29 to D408

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-Sec22b-P33-shR

Plasmid

#208359

Purpose

Mammalian expression of fluorescent N-terminally tagged shRNA resistant Sec22b-P33 mutant

Depositor

Paula Nunes-Hasler

Article

Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

Insert

Sec22b (Sec22b Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

four silent mutations (shRNA resistance), 33 aa p…

Promoter

CMV

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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Pan-Neurofascin scFv [L11A/41]

Plasmid

#206722

Purpose

Mammalian Expression of Pan-Neurofascin scFV. Derived from hybridoma L11A/41 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Pan-Neurofascin (Rattus norvegicus) recombinant scFV (Nfasc Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Tom20-mChF-AIP(TA)

Plasmid

#61513

Purpose

Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP with TA mutation

Depositor

Takanari Inoue

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

FK506 binding protein 1A (FKBP1A Human)

Use

Tags

AIP with TA mutation (see comments), Tom20, and m…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
Neuroligin-2 scFv [L107/90]

Plasmid

#206763

Purpose

Mammalian Expression of Neuroligin-2 scFV. Derived from hybridoma L107/90 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Neuroligin-2 (Mus musculus) recombinant scFV (Nlgn2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pNL3.1-Erg

Plasmid

#182706

Purpose

Express Erg enhancer in mammalian cells

Depositor

Robert Rowe

Article

Wang et al Cell Rep. 2022 Apr 5;39(1):110587. doi:

Insert

Enhancer of Erythroblast transformation-specific-Related Gene (Erg Mouse)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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Parvalbumin scFv [L114/3]

Plasmid

#182072

Purpose

Mammalian Expression of Parvalbumin scFv. Derived from hybridoma L114/3.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Parvalbumin (Rattus norvegicus) (Pvalb Mouse)

Use

Tags

HA, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-CIB1-mCerulean-Rab11

Plasmid

#140574

Purpose

For use in light-induced protein inactivation through the interaction with CRY2

Depositor

Heidi Hehnly

Article

Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

Inserts

CIB1 (CIB1 Mustard Weed)
Rab11A (RAB11A Human)

Use

Tags

mCerulean

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pOTTC810 - pAAV-EF1a-CRTsigpep-GCaMP3 (D324G+D360G+397G+D435G 10.19)-KDEL

Plasmid

#63885

Purpose

An AAV packaging vector that expresses ER-retained low-affinity GCaMP3 variant under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

Insert

ER-localized low-affinity GCaMP3(10.19)

Use

AAV

Tags

Expression

Mutation

Promoter

EF1a

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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FLINC-AKAR1-CAAX

Plasmid

#87705

Purpose

Fluorescent biosensor for super-resolution imaging of PKA activity; targeted to plasma membrane.

Depositor

Jin Zhang

Article

Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

Insert

FLINC-AKAR1-CAAX

Use

Tags

C-terminal targeting sequence from Kras

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pET21a-Med25-VBD-6His

Plasmid

#64773

Purpose

Bacterial expression of His-tagged Med25-VBD

Depositor

Ronald Conaway, Joan Conaway

Article

Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

Insert

Med25-VBD (MED25 Human)

Use

Tags

Expression

Bacterial

Mutation

insert is 394-543 only

Promoter

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
NCKX4 scFv [N414/25]

Plasmid

#190553

Purpose

Mammalian Expression of NCKX4 scFV. Derived from hybridoma N414/25.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

NCKX4 (Homo sapiens) recombinant scFV (Slc24a4 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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3322_pETcon_SARS2_Omicron-BA2

Plasmid

#184409

Purpose

yeast surface display of the SARS-CoV-2 Omicron BA.2 variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Omicron BA.2 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Use

Tags

HA and c-Myc

Expression

Yeast

Mutation

G339D+S371F+S373P+S375F+T376A+D405N+R408S+K417N+N…

Promoter

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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mcry1K31R HA tag

Plasmid

#110297

Purpose

mammalian expression of full length mouse CRY1 mutant K31R

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

mcry1 K31R (Cry1 Mouse)

Use

Tags

HA tag

Expression

Mammalian

Mutation

31 Lysines are changed to Argenine

Promoter

CMV

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast POLD1

Plasmid

#160805

Purpose

Express POLD1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

POLD1 (POLD1 Human)

Use

Retroviral

Tags

Expression

Mutation

Codon optimized C-terminal 230 amino acids

Promoter

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pEX-CMV-SP-YFP-STM1(24-685) EF hand 3 mut (A79K, N80D, D82G)

Plasmid

#18865

Depositor

Tobias Meyer

Article

Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

Insert

Stromal interaction molecule 1 (STIM1 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

3 mutations (A79K, N80D, D82G) in the EF hand of …

Promoter

Available since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

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SPHKAP scFv [L131/17]

Plasmid

#190511

Purpose

Mammalian Expression of SPHKAP scFV. Derived from hybridoma L131/17.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

SPHKAP (Mus musculus) recombinant scFV (Sphkap Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pSF4 TetCMV intron 20xGCN4 Renilla Rh1 STOP 24xMS2v5 SV40 CTE polyA

Plasmid

#105000

Purpose

Expresses ST-Renilla-Rh1 calibration reporter in mammalian cells; note plasmid contains 24xGCN4 repeats

Depositor

Jeffrey Chao

Article

Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

Insert

Renilla luciferase

Use

Tags

24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …

Expression

Bacterial and Mammalian

Mutation

Promoter

TetCMV

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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