Addgene

We narrowed to 35,149 results for: lat

Showing: 2681 - 2700 of 35149 results

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ST-SBP-EGFP_puromycin

Plasmid

#65270

Purpose

ST reporter only ( no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

truncated sialyltransferase (ST6GAL1 Human)

Use

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3 14 mut B-Myb

Plasmid

#25967

Depositor

Rob Lewis

Article

Johnson et al J Biol Chem. 2002 Feb 8. 277(6):4088-97.

Insert

B-Myb (Mybl2 Mouse)

Use

Tags

Flag

Expression

Bacterial

Mutation

Changed threonines 267, 408, 443, 447 490, 497, 5…

Promoter

Available since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only
HB901: pMVP (L3-L2) polyA

Plasmid

#121746

Purpose

pMVP L3-L2 entry plasmid, contains human growth hormone polyadenylation signal (polyA) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Polyadenylation signal (polyA)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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ATF4 11: 5’ATF4.uORF2.GFP

Plasmid

#21858

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

upstream ORF2 (uORF) of ATF4 fused to EGFP

Promoter

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pCLXSN-HA-CYLD

Plasmid

#27556

Depositor

Shao-Cong Sun

Article

Reiley et al Mol Cell Biol. 2005 May . 25(10):3886-95

Insert

CYLD (CYLD Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

R156G and G179R

Promoter

Available since

Aug. 30, 2011

Availability

Academic Institutions and Nonprofits only
pBbdCas9S(RBSmut)_Psyn-sgRNAflaB

Plasmid

#149588

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30, Psyn

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-TYK2V362F-VSV

Plasmid

#139345

Purpose

Expression of TYK2 V362F protein (rs2304256)

Depositor

Sandra Pellegrini

Article

Gauzzi et al J Biol Chem. 1996 Aug 23;271(34):20494-5

Insert

TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)

Use

Tags

VSV

Expression

Mammalian

Mutation

V362F

Promoter

CMV

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pET-30a(+)-Syk-tSH2

Plasmid

#111269

Purpose

express murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form, in E.coli strain Rosetta 2 (DE3)

Depositor

Carol Post

Article

Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

Insert

murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form (Syk Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10.1-Pct5.1-crRNA(dadR)

Plasmid

#191634

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer)

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSilencer2.1-U6-NL8F100

Plasmid

#190852

Purpose

For mammalian expression of NL8F100, a dual-specificity aptamer that binds OGT and β-catenin. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker.

Depositor

Gerald Hart

Article

Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

Insert

NL8F100 (A Dual-Specificity aptamer targeting OGT and β-catenin)

Use

Affinity Reagent/ Antibody and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSico-shRheb

Plasmid

#81087

Purpose

conditional expression of an shRNA targeting murine Rheb

Depositor

Angelique Bordey

Article

Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

Insert

Rheb shRNA (Rheb Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro NM23 H1 WT

Plasmid

#11364

Depositor

Ronald Kahn

Article

Zhu et al Proc Natl Acad Sci U S A. 1999 Dec 21. 9

Insert

NM23 H1 (NME1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 1, 2006

Availability

Academic Institutions and Nonprofits only
ATF4 14: 5’ATF4.uORF1&2AUA.GFP

Plasmid

#21861

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

AUG codons of uORFs1&2 were converted to AUA

Promoter

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pSilencer2.1-U6-3JB1R+6

Plasmid

#190842

Purpose

For mammalian expression of 3JB1R+6, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.

Depositor

Gerald Hart

Article

Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

Insert

3JB1R_AP3+6bp (A Dual-Specificity aptamer targeting OGT and GFP)

Use

Affinity Reagent/ Antibody and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pBbdCas9S(RBSmut)_Psyn-sgRNAftsI

Plasmid

#149590

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi ftsI

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAftsI

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30, Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCMX-PL1-WDR5

Plasmid

#59970

Purpose

Mammalian Expression of WDR5

Depositor

Debu Chakravarti

Article

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Insert

WDR5 (WDR5 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only
pBSSK p85 alpha (delta SH3)

Plasmid

#13430

Depositor

Ronald Kahn

Article

Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

Insert

p85 alpha (delta SH3) (PIK3R1 Human)

Use

Tags

FLAG

Expression

Bacterial

Mutation

SH3 domain replaced by flag tag.

Promoter

Available since

Nov. 1, 2006

Availability

Academic Institutions and Nonprofits only
Str-KDEL_ManII-SBP-tagBFP

Plasmid

#65254

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Use

Tags

Streptavidin Binding Protein (SBP) and tagBFP

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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pUAST-attB-Flag-dTSC2 WT

Plasmid

#111807

Purpose

Insect Expression of dTSC2

Depositor

Jun Hee Lee

Article

Kim et al Int J Mol Sci. 2015 Mar 27;16(4):7015-26

Insert

dTSC2 (gig Fly)

Use

Tags

3xFlag

Expression

Insect

Mutation

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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2Bc-T MBP_BoMoC(ed)_I770A_6xH

Plasmid

#185711

Purpose

Recombinant B. mori truncated R2 RT expression

Depositor

Kathleen Collins

Article

Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

Insert

B. mori truncated R2 RT

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Changed I770 to A

Promoter

T7

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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