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Plasmid#161523PurposeGateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE8e mediated A-G base editingDepositorInsertABE8e-zSpCas9(D10A)
UseCRISPR; Gateway compatible abe8e-zspcas9(d10a) en…TagsExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-YE1-BE3-FNLS-CMV-mCherry
Plasmid#154005PurposeExpress YE1-BE3-FNLSDepositorInsertYE1-BE3-FNLS
UseCRISPRTagsFLAGExpressionMutationPromoterCMVAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GACh1.0
Plasmid#106072Purposeexpress the genetically-encoded fluorescent ACh indicator GACh1.0 in mammalian cellsDepositorInsertGPCR activation based ACh sensor (GACh) 1.0
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSP1101
Plasmid#65773PurposeHuman expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)DepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationD1135V, G1218R, R1335E, and T1337R mutations in C…PromoterCMV & T7Available sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-TEAD4-IDR
Plasmid#166451PurposeExpresses fusion of mCherry and TEAD4-IDRDepositorInsertmCherry, TEAD4-IDR(89-230) (TEAD4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ266E
Plasmid#161521PurposeGateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 PmCDA1 mediated C-T base editingDepositorInsertzSpRYCas9(D10A)-PmCDA1-UGI
UseCRISPR; Gateway compatible zsprycas9(d10a)-pmcda1…Tags3X FLAG, NLSExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-N1-YAPS127A-deltaCC
Plasmid#166459PurposeExpresses fusion of mEGFP and YAPS127A-deltaCC (delta298-359)DepositorInsertmEGFP, YAPS127A-deltaCC (YAP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-N1 YAPS127A- L308E
Plasmid#166462PurposeExpresses fusion of mEGFP and YAPS127A- L308EDepositorInsertmEGFP, YAPS127A- L308E (YAP1 Human)
UseTagsExpressionMammalianMutationYAPS127A- L308EPromoterAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-N1 YAPS127A-deltasIDR
Plasmid#166460PurposeExpresses fusion of mEGFP and YAPdeltasIDR (delta360-449)DepositorInsertmEGFP, YAPS127A-deltasIDR (YAP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-N1 YAPS127A-deltasTAD
Plasmid#166461PurposeExpresses fusion of mEGFP and YAPdeltasTAD (delta450-504)DepositorInsertmEGFP, YAPS127A-deltasTAD (YAP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
PITX3-eGFP-PGK-Puro
Plasmid#31942DepositorInsertPITX3 (PITX3 Human)
UseTargeting donorTagseGFPExpressionMutationPromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Lifect-7-pr-mEos2 (mEos2-A69T)
Plasmid#99229PurposeMammalian expression of the primed conversion capable protein pr-mEos2 (mEos2-A69T) as a Lifeact fusion.DepositorInsertpr-mEos2
UseTagsLifeactExpressionMammalianMutationA69T for primed conversion applicationsPromoterCMVAvailable sinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ262B-ABE8e
Plasmid#161524PurposeGateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 ABE8e mediated A-G base editingDepositorInsertTadA8e-zSpRYCas9(D10A)
UseCRISPR; Gateway compatible tada8e-zsprycas9(d10a)…TagsExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2-Sox9 T236A
Plasmid#111455PurposeTransient expression of Flag-tagged human Sox9 with T236A mutationDepositorInsertSox9 (SOX9 Human)
UseTagsFlagExpressionMammalianMutationT236A mutationPromoterCMVAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
FUT8-Dox
Plasmid#124631PurposeDoxycycline-inducible FUT8 expression in mammalian cellsDepositorInsertsAlpha-(1,6)-fucosyltransferase
Tetracycline repressor A3 mutant
TagFP635
UseTagsExpressionMammalianMutationPromoterTRE-tight and hEF1aAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJS699
Plasmid#168779PurposeEncodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) and can be combined with pJS697 through homologous recombinationDepositorInsertSpike RBD (S betacoronavirus)
UseTagsCmycExpressionMutationN343QPromoterGAL1Available sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shSTIM1
Plasmid#89816PurposeKnockdown human STIM1 expressionDepositorInsertSTIM1 shRNA (STIM1 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2-Sox9 T240A
Plasmid#111457PurposeTransient expression of Flag-tagged human Sox9 with T240A mutationDepositorInsertSox9 (SOX9 Human)
UseTagsFlagExpressionMammalianMutationT240APromoterCMVAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-mKikGR1 (mKikGR1-V69T)
Plasmid#99227PurposeBacterial expression of the primed conversion capable protein pr-mKikGR1 (mKikGR1-V69T).DepositorInsertpr-mKikGR1
UseTagsExpressionBacterialMutationmonomeric variant with mutation V69T (Eos notatio…PromoterT7Available sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2-Sox9 T236A/T240A
Plasmid#111458PurposeTransient expression of Flag-tagged human Sox9 with T236A/T240A mutationDepositorInsertSox9 (SOX9 Human)
UseTagsFlagExpressionMammalianMutationT236A and T240APromoterCMVAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only