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Plasmid#170290PurposeFor generating the conditional transgenic mice expressing BRET-FRET hybrid biosensor of ERK.DepositorInsertlox-mCherry-lox-hyBRET-ERK-NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGAN147
Plasmid#129202Purposeyeast transcriptional reporter of aggregating proteins (yTRAP) to detect protein aggregation, Gateway backboneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterSUP35Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS-nlstdTomato
Plasmid#132985Purposefull length of nuclear localized tdTomato in pCS expression vectorDepositorInserttdTomato
UseTagsNLS and mycExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-EGFP-eDHFR(69K6)
Plasmid#178850PurposeMammalian expression of EGFP-eDHFR(69K6)DepositorInsertEGFP-eDHFR(69K6)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCas9-VRER_2A_GFP
Plasmid#75475PurposeCas9 VRER variant that detects NGCG PAM, combined with 2A_GFP for expression controlDepositorInsertCas9-VRER
UseCRISPRTags2A_GFPExpressionMammalianMutationD1135V, G1218R, R1335E, T1337RPromoterCAGAvailable sinceMay 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPaxillin-Electra1-N1
Plasmid#179484PurposeExpresses paxillin fused to blue fluorescent protein Electra1DepositorInsertpaxillin-Electra1
UseTagsElectra1ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-mTagBFP2
Plasmid#184940PurposeER Visualization (OSER assay) Expresses mTagBFP2 in fusion with N-terminal part of p450DepositorInsertCytERM-mTagBFP2
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
819 pGEX2T PTEN C124S
Plasmid#10745DepositorInsertPTEN (PTEN Human)
UseTagsGSTExpressionBacterialMutationC124SPromoterAvailable sinceSept. 27, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-Cerulean-SEC4
Plasmid#18844DepositorInsertSEC4 (SEC4 Budding Yeast)
UseTagsCeruleanExpressionYeastMutationPromoterAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGAN213
Plasmid#129270PurposeControl for measuring background levels of yTRAP reporter proteinDepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromotermin pCYC1Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
p5E-6xgata2aECE
Plasmid#132969Purposegata2a endothelial enhancer (x6) in p5E Gateway vectorDepositorInsertgata2a endothelial enhancer (x6)
UseGateway vectorTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACEC3GH (Apr)
Plasmid#110085PurposeReplicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR4_FLAG/HA_FUS_His6
Plasmid#26365DepositorInsertFUS (FUS Human)
UseEntry vectorTagsFLAG/HA and His6ExpressionMutationdelta 527 (stop codon)PromoterAvailable sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSK234
Plasmid#131149Purposedual yTRAP, Gateway destination vector of aTF2 fusion reporting on mKate2DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionYeastMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVPSB_empty
Plasmid#192170PurposepVPSB backbone expression vectorDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationNAPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-EBFP2
Plasmid#184939PurposeER Visualization (OSER assay) Expresses EBFP2 in fusion with N-terminal part of p450DepositorInsertCytERM-EBFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-PAmCherry1-MCS
Plasmid#60608PurposeCloning PAmCherry1DepositorInsertPAmCherry1
UseCloningTagsExpressionMutationPromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSK260
Plasmid#129252Purposedual yTRAP, NM fusion to aTF1 reporting on [PSI+] state with mKate2 reporterDepositorInsertSup35 NM yTRAP sensor reporting on mKate2
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
p6607 MSCV-IP N-HAonly PTPN18
Plasmid#35007DepositorInsertPTPN18 (PTPN18 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterMSCV LTRAvailable sinceFeb. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pME-APEX2-mCherry
Plasmid#188944PurposeMiddle entry vector for Gateway cloning containing APEX2 tagged to mitochondria and nuclei, and cytoplasmic mCherryDepositorInsertmito-APEX2_p2A_APEX2-H2B_p2A_mCherry
UseMiddle entry vector for gateway (l1-l2)TagsExpressionMutationPromoterAvailable sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only