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Plasmid#206159PurposeLuciferase reporter construct containing 4 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350DepositorInsert4X PRE TK luciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS2E
Plasmid#55169PurposeEmpty vector, integration at lacA, ampr, mlsrDepositorTypeEmpty backboneUseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoternoneAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSL0375 (His-MBP-TniQ_Cas8_Cas7_Cas6)
Plasmid#130640PurposeExpresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on TniQ for purification.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6
UseCRISPR; TransposonTagsHis10-MBP-TEVsite on TniQExpressionBacterialMutationPromoterT7Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSL0812 (pCRISPR(BbsI)_Vch)
Plasmid#200898PurposeExpresses V. cholerae CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.DepositorInsertVchCRISPR (entry)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
MGAT1-GFP
Plasmid#163647PurposeExpresses GFP tagged MGAT1 in mammalian cellsDepositorInsertalpha-1,3-mannosyl-glycoprotein beta-1, 2-N-acetylglucosaminyltransferase (MGAT1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSL0284 (pQCascade_entry)
Plasmid#130635PurposeExpresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array contains two BsaI sites for spacer cloning.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterT7Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-1
Plasmid#72680PurposeSame as pORTMAGE-2, but with a non-optimized RBS before mutant MutL, thus lower level of expression of the enzyme.DepositorInsertmutL E32K
UseTagsnoneExpressionBacterialMutationlower strength RBS before mutL E32K allele than i…PromoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-sL7-Cre-HA-WPRE
Plasmid#204488PurposeFor production of driver AAV virus for Purkinje cell-specific expressionDepositorInsertCre
UseAAV, Cre/Lox, and Mouse TargetingTagsExpressionMammalianMutationPromotersL7Available sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL0762 (pQCascade_crRNA-nt)
Plasmid#130636PurposeExpresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a non-targeting guide RNA.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(non-targeting)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterT7Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28C-mCherry-DDX4LCD
Plasmid#204408PurposeE. coli expression of DDX4 low-complexity domain (1-236) with an N-terminal mCherry tagDepositorInsertDDX4(1-236) (DDX4 Human)
UseTags6x-His; mCherryExpressionBacterialMutationPromoterT7Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL0828 (pQCascade_crRNA-4)
Plasmid#130637PurposeExpresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a guide RNA that targets lacZ.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(target-4)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterT7Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHM123DEnv
Plasmid#137668PurposeExpress HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)DepositorInsertHIV-1 Envelope Glycoprotein
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP1-N1-SialT
Plasmid#87324PurposeTo express SialT-AcGFP1 in mammalian cells, SialT encodes the full length coding sequence of β-galactosamide α-2,6-sialyltransferaseDepositorInsertST 6 beta-galactoside alpha-2,6-sialyltransferase 1 (ST6GAL1 Human)
UseTagsAcGFP1ExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSL3936 (pDonor(AAVS1)_Pse)
Plasmid#200903PurposeEncodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a SA-T2A-PuroR-T2A-eGFP cargo. Total transposon size = 2,024 bp.DepositorInsertMini Tn7016 (AAVS1 NGS)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL0374 (His-MBP-Cas8_Cas7_Cas6)
Plasmid#130639PurposeExpresses V. cholerae CAST Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on Cas8 for purification.DepositorInsertVchCas8, VchCas7, VchCas6
UseCRISPR; TransposonTagsHis10-MBP-TEVsite on Cas8ExpressionBacterialMutationPromoterT7Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
cfmTurquoise-N
Plasmid#139284PurposeCys-free mTurquoise (improved ECFP) for extracellular FRET analysis. This variant contains the same set of mutations (C48S and C70M ) as cfSGFP2. Molecular brightness is similar to mTurquoise.DepositorInsertcfmTurquoise
UseTagsMulti-cloning sitesExpressionMammalianMutationCys48Ser and Cys70/MetPromoterAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHM024Env
Plasmid#137691PurposeExpress HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)DepositorInsertHIV-1 Envelope Glycoprotein
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSL0747 (pDonor_L-MmeI)
Plasmid#130647PurposeEncodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated left end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.DepositorInsertVchCAST donor DNA (L*)
UseCRISPR; TransposonTagsExpressionBacterialMutationTGTTGATG --> TGTTGGAGPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSL0746 (pDonor_R-MmeI)
Plasmid#130646PurposeEncodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated right end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.DepositorInsertVchCAST donor DNA (R*)
UseCRISPR; TransposonTagsExpressionBacterialMutationTGTTGATA --> TGTTGGAAPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMIG-Flag-RIPK1-D324A
Plasmid#102678Purposeretroviral expression plasmid for RIPK1-D324ADepositorInsertRIPK1 (RIPK1 Human)
UseRetroviralTagsFlag and IRES-GFPExpressionMammalianMutationCHANGE Glutamate 324 to AlaninePromoterunknownAvailable sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only