Addgene

We narrowed to 15,769 results for: puromycin

Showing: 2661 - 2680 of 15769 results

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lentiguide-puro-wt-ltr

Plasmid

#182635

Purpose

Expresses guide RNA, contains intact LTR

Depositor

Jakob Reiser

Article

Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

Insert

S. pyogenes sgRNA cassette

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pMKO.1-Zfp423

Plasmid

#35972

Depositor

Bruce Spiegelman

Article

Gupta et al Nature. 2010 Mar 3. ():.

Insert

zfp423 shRNA (Zfp423 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
p667-UBC-AAK1-V5-miniTurbo_IDG-K

Plasmid

#135255

Purpose

Gateway destination clone of AAK1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

AAK1-V5-miniTurbo (AAK1 Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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LentiGuide-BC-CMV-Puro

Plasmid

#127169

Purpose

PCR template for CMV-puro resistance cassette

Depositor

Paul Blainey

Article

Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

Insert

None

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pMK1200

Plasmid

#84219

Purpose

Lentiviral expression of shRNAs, compatible with double-shRNA expression, EF1alpha promoter, mCherry

Depositor

Martin Kampmann, Jonathan Weissman

Article

Kampmann et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Type

Empty backbone

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pLPCX mCER-Prx1

Plasmid

#158227

Purpose

Genetically encoded HomoFRET-based probe to monitor peroxiredoxin1 (Prx1) oligomerization (for stable cell line generation)

Depositor

Tobias Dick

Article

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

mCER-Prx1

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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p663-UBC-V5-GSK3B_IDG-K

Plasmid

#158166

Purpose

Gateway destination clone of V5-GSK3B (human) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

V5-GSK3B (GSK3B Human)

Use

Lentiviral; Gateway destination

Tags

V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-H1-SGIPZ_NF1 sh1miR

Plasmid

#119206

Purpose

knockdown of NF1

Depositor

Helen Morrison

Article

Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

Insert

sh1miR targeting NF1 (NF1 Rat, Human, Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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lentiGuide-Puro-Chen-tracrRNA_2xBsmBI

Plasmid

#196710

Purpose

Backbone for cloning single guides (Chen tracrRNA PMID: 24360272) or guide libraries with untranscribed barcodes and a tracrRNA of choice

Depositor

Bernhard Schmierer

Article

Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pTS1106_Tier3(Lenti)

Plasmid

#169661

Purpose

Tier-3 vector for stable integration of up to three cassettes via lentiviral transduction (PCMV-5'LTR-Psi-gag-env-A1::A2::A3-WPRE-3'LTR)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLPCX mCER-Prx2

Plasmid

#158225

Purpose

Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (for stable cell line generation)

Depositor

Tobias Dick

Article

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

mCER-Prx2

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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LPCX Smad3 deltaSSVS

Plasmid

#12639

Depositor

Rik Derynck

Article

Choy et al J Cell Biol. 2000 May 1. 149(3):667-82.

Insert

Smad3 (SMAD3 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Last 4 aa's deleted.

Promoter

Available since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
pIRES.Puro.EGFP

Plasmid

#24176

Depositor

Robert Sobol

Article

Jelezcova et al Mutat Res. 2010 Jan 22. ():.

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

x

Promoter

Available since

April 9, 2010

Availability

Academic Institutions and Nonprofits only
pALPS puro miR30-STAT1

Plasmid

#117160

Purpose

Target site ATATCCAGTTCCTTTAGGGCC

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pN-PURO-PTP

Plasmid

#51711

Purpose

contains an N-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.

Depositor

Arthur Gunzl

Article

Schimanski et al Eukaryot Cell. 2005 Nov;4(11):1942-50.

Type

Empty backbone

Use

T. brucei genome integration vectors

Tags

PTP (ProtC-TEV-ProtA)

Expression

Mutation

Promoter

Available since

March 19, 2014

Availability

Academic Institutions and Nonprofits only

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pBRIT HA/FLAG

Plasmid

#17519

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Type

Empty backbone

Use

Cre/Lox and Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
PSICORhp-mp21(v2)

Plasmid

#26272

Depositor

Rudolf Jaenisch

Article

Hanna et al Nature. 2009 Dec 3. 462(7273):595-601.

Insert

p21 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 23, 2010

Availability

Academic Institutions and Nonprofits only
pBabe-Kras G15A

Plasmid

#58898

Purpose

Expresses human Kras with a G15A mutation

Depositor

Channing Der

Insert

KRAS (KRAS Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

G15A mutation

Promoter

Available since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only
pLKO-shCIAP2-A

Plasmid

#44130

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

CIAP2

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2013

Availability

Academic Institutions and Nonprofits only
Lentihair s1GFP

Plasmid

#8451

Depositor

Bob Weinberg

Article

Stewart et al RNA 2003 Apr;9(4):493-501.

Insert

siRNA against GFP

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2005

Availability

Academic Institutions and Nonprofits only