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Plasmid#29665DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only
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pET-6xHis-(pos9)GFP
Plasmid#89247PurposeExpresses GFP with +9 theoretical charge in E.ColiDepositorInsertHis6-(pos9)-GFP
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETDuet RagA(D130N)-RagC
Plasmid#99642PurposeBacterial expression of RagA(D130N)-RagCDepositorUseTagsHis tagExpressionBacterialMutationD130NPromoterAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
Mucin MUC1-5TR-pET15b
Plasmid#174694PurposePlasmid expressing five copies of the MUC1 tandem repeat (MUC1-5TR)DepositorInsertMUC1-TR
UseTagshexahistidine (HIS) tagExpressionBacterialMutationnonePromoterT7 PromoterAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETDuet RagA-RagC(D181N)
Plasmid#99643PurposeBacterial expression of RagA-RagC(D181N)DepositorUseTagsHis tagExpressionBacterialMutationD181NPromoterAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-21a(+)-AK1-His
Plasmid#118977PurposeBacterial expression plasmid for Adenylate kinase isoenzyme 1DepositorInsertadenylate kinase isoenzyme 1 AK1 (AK1 Chicken)
UseTags6x-HisExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymYPET-URA3
Plasmid#168056PurposeReplaces Addgene plasmid 118460. Yeast tagging vector to create a gene fusion with yeast optimized mYPET with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertymYPET
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET22-His-TEV-DOT1L
Plasmid#89610PurposeBacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L residues 1-416DepositorInsertDot1L (DOT1L Human)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET_His6-SUMO-TEV-TtCsm6
Plasmid#172486PurposeBacterial expression of codon-optimized His-SUMO-TtCsm6 with a TEV protease cleavage site.DepositorInsertT. thermophilus Csm6 (codon optimized) (csm6 Synthetic)
UseTagsHis-SUMO-TEVExpressionBacterialMutationPromoterT7 promoter and lac operatorAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMG046 pETARA-CK1α-His
Plasmid#154074PurposeExpresses GST_CK1α (human CK1α as a fusion protein with GST and His- tags) in E.coliDepositorInsertCSNK1A1 (CSNK1A1 Human)
UseTagsGST and His6ExpressionBacterialMutationPromoterT7Available sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-EIN-FTO(32-505)
Plasmid#186466PurposeExpresses human RNA demythelase protein FTO 32-505 amino acid sequence with N-terminal fusion with the N-domain of EI ( e.coli protein)DepositorInsertFat mass obesity associated protein (FTO Human)
UseTags6 Histidine-EIN (N-terminal domain of E.coli Enzy…ExpressionBacterialMutationamino acid 32-505PromoterT7Available sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-His-TEV-NQO1
Plasmid#119058PurposeFor expression in E.coli and affinity purification of N-terminally His tagged NQO1DepositorInsertNAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
UseTags6His, TEV siteExpressionBacterialMutationPromoterT7Available sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
[pSK104] pET28 SLC38A9N-His
Plasmid#136142Purposebacterial expression of SLC38A9 N term fragment (1-119)DepositorInsertSLC38A9 N term fragment (1-119) (SLC38A9 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-Caspase-4_enzymatic-domain
Plasmid#183381PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertCaspase-4 (AA94-377) (CASP4 Human)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)_NAD-Snifit
Plasmid#113658PurposePlasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring NAD+ concentrationDepositorInsertNAD-Snifit
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b-Casp8 delta DED
Plasmid#11827DepositorInsertCaspase 8 minus Death Effecter Domain (CASP8 Human)
UseTags6xHisExpressionBacterialMutationMinus Death Defecter Domain (aa 1-216)PromoterAvailable sinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET-24a-ELP[V-150]
Plasmid#67015PurposeExpresses ELP[V-150] in E. coliDepositorInsertELP[V-150]
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-MCN-10His-StayGold
Plasmid#211360PurposeBacterial expression of the bright and photostable StayGold fluorescent protein. Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.DepositorInsertStayGold
UseTags10xHis-tagExpressionBacterialMutationPromoterT7Available sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Endosomal Escape Control
Plasmid#186552PurposeNegative Control confirming the need for a CPP peptide in mediating internalizationDepositorInsert6H-ETA-GFP
UseTags6xHis and EGFPExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Internalization and Endosomal Escape
Plasmid#186551PurposeInternalization and Endosomal Escape to effectively route a protein cargo to the cytosol of cellsDepositorInsert10R-8His-ETA-EGFP
UseTags8xHis and EGFPExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only