Addgene

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pGEX4T-1 Slp2-a E11A/R32A

Plasmid

#40063

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Slp2-a E11A/R32A (Sytl2 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Glutamate 11 to Alanine, Arginine 32 to Alanine

Promoter

Tac

Available since

Nov. 9, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 Slp2-a C2A

Plasmid

#40052

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Slp2-a C2A (Sytl2 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

C2A domains

Promoter

CMV promoter

Available since

Nov. 9, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 hSlp4-a K>Q

Plasmid

#40044

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a K>Q (SYTL4 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Lysine 410,412,416,564,566,571, 572 to Glutamine

Promoter

CMV promoter

Available since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 hSlp4-5-4a

Plasmid

#40045

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-5-4a (SYTL4 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Slp4 linker substituted by Slp5 linker domain (s…

Promoter

CMV promoter

Available since

Oct. 26, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 hSlp4-a W118S

Plasmid

#40042

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a W118S (SYTL4 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Tryptophane 118 to Serine

Promoter

CMV promoter

Available since

Oct. 17, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 hSlp4-a V21A

Plasmid

#40041

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a V21A (SYTL4 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Valine 21 to Alanine

Promoter

CMV promoter

Available since

Oct. 17, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 hSlp4-a SHD

Plasmid

#40034

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a SHD (SYTL4 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

SHD domain (1-143)

Promoter

CMV promoter

Available since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-hAT-4_Crp

Plasmid

#217168

Purpose

Mammalian expression vector for expression of hAT-4_Crp transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

hAT-4_Crp transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-hAT-1_AMi

Plasmid

#217170

Purpose

Mammalian expression vector for expression of hAT-1_Ami transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

hAT-1_AMi transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Mariner-2_AMi

Plasmid

#217171

Purpose

Mammalian expression vector for expression of Mariner-2_Ami transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

Mariner-2_AMi transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-hAT-13_AMi

Plasmid

#217172

Purpose

Mammalian expression vector for expression of hAT-13_Ami transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

hAT-13_AMi transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pEZYeGFP-SARS-CoV-2E

Plasmid

#224580

Purpose

To express a GFP tagged version of the Envelope protein of SARS-CoV-2 in mammalian cells

Depositor

Isabel Mérida

Article

Avila-Flores et al Int J Mol Sci. 2023 Aug 14;24(16):12793.

Insert

SARS-CoV-2E (E SARS-CoV-2)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Harbinger-1_Crp-Myb

Plasmid

#217219

Purpose

Mammalian expression vector for expression of transposase (CMV promoter)

Depositor

Yong E Zhang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

Harbinger-1_Crp myb like protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_MBP_RBFOX2

Plasmid

#224292

Purpose

MBP-RBFOX2 protein purification from bacteria

Depositor

Chuan He

Article

Dou et al Nat Cell Biol. 2023 Sep;25(9):1359-1368.

Insert

RNA binding fox-1 homolog 2 (RBFOX2 Human)

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pC0043-PspCas13b-gRNA non target

Plasmid

#223698

Purpose

gRNA control for dPspCas13b-FTO

Depositor

Chuan He

Article

Wei et al Science. 2022 May 27;376(6596):968-973.

Insert

gRNA target sequence for luciferase control

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV CMV-DIO-(mCherry-U6)-shRNA(anti-Crh)

Plasmid

#214732

Purpose

Expresses shRNA against CRH, marked with mCherry, in infected and cre positive cells

Depositor

Félix Leroy

Article

de Leon Reyes et al Cell. 2023 Sep 14;186(19):4152-4171.e31.

Insert

anti-Crh shRNA / mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-p62-TIR-AAA

Plasmid

#187901

Purpose

Mammalian cell expression of a triple mutant of p62: P228A, E230A, P232A

Depositor

Erika Holzbaur

Article

Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

Insert

SQSTM1 (SQSTM1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Triple alanine mutant: P228A, E230A, P232A

Promoter

CMV

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Gα12-133-NLuc

Plasmid

#207156

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA12 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

Depositor

Tom Van Loy, Dominique Schols

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNA12 (GNA12 Human)

Use

Tags

Expression

Mammalian

Mutation

NLuc inserted at position A(133)F

Promoter

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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AID∆C-Cas9n-UGI-NLS

Plasmid

#198890

Purpose

Express AID(1-188) BE3-based base editor

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

Actication induced deaminase (AICDA Human)

Use

Tags

NLS

Expression

Mammalian

Mutation

Deletion of amino acids 189-198

Promoter

CMV

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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Halo-tagged Sox17FNV

Plasmid

#206391

Purpose

Expresses Halo-tagged mouse SOX17FNV in mammalian cells, for single-molecule tracking.

Depositor

Ralf Jauch

Article

Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

Insert

Sox17FNV (Sox17 Mouse)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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