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Plasmid#90236PurposeLentiviral vector to express H2B-mRuby2 under PGK promoter (With Hygromycin Resistance)DepositorInsertH2B
UseLentiviralTagsmRuby2ExpressionMammalianMutationPromoterPGKAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL6
Plasmid#48746PurposeExpression of luciferase driven by mPer2 promoter region (bases -1128 to +2129 with respect to transcription start site at +1)DepositorInsertmPer2 promoter/enhancer region (-1128 to +2129)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pActin-Electra2
Plasmid#184942Purposebeta-actin labelling with blue fluorescent protein Electra2DepositorInsertElectra2-beta-actin
UseTagsElectra2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-Electra2
Plasmid#179477PurposeER Visualization (OSER assay) Expresses Electra2 in fusion with N-terminal part of p450DepositorInsertCytERM-Electra2
UseTagsElectra2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTol2-nbt-Electra1-P2A-mScarlet
Plasmid#196113Purposezebrafish expression of nbt-Electra1-P2A-mScarletDepositorInsertnbt-Electra1-P2A-mScarlet
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFLAG_attP
Plasmid#110095PurposeVector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.DepositorTypeEmpty backboneUseTags3xFLAGExpressionBacterialMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
qMaLioffG
Plasmid#212825Purposegreen fluorescent FLIM indicator for endogenous ATP imagingDepositorInsertqMaLioffG
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-CBS (WT)
Plasmid#182923PurposeMammalian expression plasmid for human CBSDepositorInsertCystathionine Beta-Synthase (CBS Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMA_Int
Plasmid#110096PurposeE. coli replicative vector which contains the mycophage integrase gene required for the attP/attB integration. Is co-transformed in trans together with pFLAG_attP, but cannot replicate in mycobacteria, and is thus lost shortly after electroporation.DepositorInsertmycophage integrase
UseRequired for transformation of attb integrating v…TagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCX-SponGee
Plasmid#134775Purposemammalian expression of SponGee, a buffer of cGMP that prevents downstream signalingDepositorInsertSponGee
UseTagsFLAG and mRFPExpressionMammalianMutationPromoterCAGAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-Cerulean-GOS1
Plasmid#18846DepositorInsertGOS1 (GOS1 Budding Yeast)
UseTagsceruleanExpressionYeastMutationPromoterAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
811 pSG5L HA PTEN C124S
Plasmid#10744DepositorInsertPTEN (PTEN Human)
UseTagsHAExpressionMammalianMutationC124SPromoterAvailable sinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCS2-FLAG-hEcto/Tif1g
Plasmid#20902DepositorInsertEctodermin (TRIM33 Human)
UseTagsFLAGExpressionMammalianMutationN-terminal deletion of 37 aminoacids; Ectodermin …PromoterAvailable sinceApril 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
882 pSG5L HA PTEN G129E
Plasmid#10746DepositorInsertPTEN (PTEN Human)
UseTagsExpressionMammalianMutationG129EPromoterAvailable sinceSept. 27, 2005AvailabilityAcademic Institutions and Nonprofits only -
Tol2pA2-drl:APEX2-mCherry
Plasmid#188945PurposePlasmid for Tol2 transgenesis expressing APEX2 tagged to mitochondria and nuclei, and cytoplasmic mCherry, driven by the draculin promoterDepositorInsertdrl:mito-APEX2_p2A_APEX2-H2B_p2A_mCherry_polyA
UseTol2 destination vector (attr4-r3)TagsExpressionMutationPromoterAvailable sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMET7-GAG-GW
Plasmid#194634PurposeGateway destination vector for mammalian expression of Virotrap bait constructsDepositorInsertp55 GAG
UseTagsPQS1ExpressionMammalianMutationPromoterSRalphaAvailable sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2-nbt-mTagBFP2-P2A-mScarlet
Plasmid#196115Purposezebrafish expression of nbt-mTagBFP2-P2A-mScarletDepositorInsertnbt-mTagBFP2-P2A-mScarlet
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSK221
Plasmid#129249Purposereverse yTRAP, tetR-responsive Neon reporterDepositorInsertpADH1(tetO2)-mNeonGreen
UseTagsExpressionYeastMutationPromoterADH1Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACEC3GH (Hyg)
Plasmid#110084PurposeReplicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
817 pSG5L HA PTEN G129R
Plasmid#10748DepositorInsertPTEN (PTEN Human)
UseTagsHAExpressionMammalianMutationG129RPromoterAvailable sinceSept. 27, 2005AvailabilityAcademic Institutions and Nonprofits only