Addgene

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OA-1050L (UASt-CasRx)

Plasmid

#132418

Purpose

Express CasRx under UASt promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Inserts

CasRx
UASt+hsp70

Use

Tags

Expression

Insect

Mutation

Promoter

UASt

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pVW294

Plasmid

#139491

Purpose

Insertion of the pGRE2 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pGRE2 24xPP7sl

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

pGRE2

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pSP569

Plasmid

#139499

Purpose

Repair DNA plasmid to insert PP7 stem loops in the endogenous STL1 locus after double strand break in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pSTL1 24xPP7sl STL1 100 - 600

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

pSTL1

Available since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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pVW295

Plasmid

#139492

Purpose

Insertion of the pALD3 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pALD3 24xPP7sl

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

pALD3

Available since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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pSP266

Plasmid

#139488

Purpose

Insertion of the pCTT1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pCTT1 24xPP7sl

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

pCTT1

Available since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pSP264

Plasmid

#139487

Purpose

Insertion of the pSTL1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pSTL1 24xPP7sl

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

pSTL1

Available since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pVW200

Plasmid

#139489

Purpose

Insertion of the pGPD1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pGPD1 24xPP7sl

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

pGPD1

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pVW293

Plasmid

#139490

Purpose

Insertion of the pHSP12 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pHSP12 24xPP7sl

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

pHSP12

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSL1211

Plasmid

#129521

Purpose

pDEF-SpCas9::GFP, For deletion of pyalba4, PyGAPDH promoter, 2nd Generation

Depositor

Scott Lindner

Article

Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

Insert

SpCas9

Use

Plasmodium, rodent-infectious species

Tags

3XFlag-NLS

Expression

Mutation

Promoter

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-K295TAG-W340A.pcDNA3-k

Plasmid

#126627

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site.

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Use

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid mutations: K295 to Amber codon and W34…

Promoter

CMV

Available since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-K295TAG-W340A-CAAX.pcDNA3-k

Plasmid

#127399

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Use

Tags

CAAX and FLAG

Expression

Mammalian

Mutation

Amino acid mutations: K295 to Amber codon and W34…

Promoter

CMV

Available since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-E322TAG-E309C-W340A.pcDNA3-k

Plasmid

#127408

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Use

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid mutations: E322 to Amber codon, E309C …

Promoter

CMV

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-E322TAG-W340A-CAAX.pcDNA3-k

Plasmid

#127405

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Use

Tags

CAAX and FLAG

Expression

Mammalian

Mutation

Amino acid mutations: E322 to Amber codon and W34…

Promoter

CMV

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI119

Plasmid

#126059

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

chloroplastic FNR transit peptide

Use

Synthetic Biology; Module 0

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI120

Plasmid

#126060

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

mitochondria Rieske iron sulphur protein (1-100 aa)

Use

Synthetic Biology; Module 0

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI123

Plasmid

#126063

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

GFP 11x3 (5 aa linker)

Use

Synthetic Biology; Module 0

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI124

Plasmid

#126064

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

GFP 11x7 (5 aa linker)

Use

Synthetic Biology; Module 0

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-E322TAG-W340A.pcDNA3-k

Plasmid

#127404

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site.

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Use

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid mutations: E322 to Amber codon and W34…

Promoter

CMV

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TetO-U2B''-fSNAPc

Plasmid

#106988

Purpose

open reading frame of U2B'' inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

UB2" (SNRPB2 Human)

Use

Tags

fSNAP

Expression

Mutation

Promoter

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNc PSM1

Plasmid

#106328

Purpose

Mammalian expression of mutant osteopontin

Depositor

Georg Weber

Article

Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

Insert

OPNc (SPP1 Human)

Use

Tags

Expression

Mammalian

Mutation

OPNc phosphorylation site mutant SGSSEEKQNAVSSEET…

Promoter

Available since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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