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Plasmid#72521PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ)DepositorInsertformylglycinamidine ribonucleotide synthetase, glutaminase subunit
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
AiP5001 RabV-CVS-N2cdeltaG-histone-EGFP
Plasmid#176283PurposeRecombinant rabies virus expressing histone-EGFPDepositorInsertH2B (H2BC11 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJK369
Plasmid#73594PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitopeDepositorInsertdelta-12 fatty acid acetylenase
UseTagsFLAG tagExpressionYeastMutationPromoterGAL10Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK545
Plasmid#74225PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ)DepositorInsertformylglycinamidine ribonucleotide synthetase, glutaminase subunit
UseTagsExpressionBacterialMutationcontains a silent mutation in Ala63 codonPromoterT7Available sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
PGR-Blue
Plasmid#68374PurposeTerminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.DepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assemblyTagsExpressionBacterialMutationPromoterpBADAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL 636
Plasmid#53015PurposeAmpR + PLtetO-1::RBS (st7) fimE::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1DepositorInsertsfimE
fimB
UseSynthetic BiologyTagsAsp terminator, RBS(st7), and T1 terminatorExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
J-SPRTNC-1
Plasmid#166521PurposescFv of a human scaffold targeting SprT-like N-terminal domain. Antigen coverage aa 1-216 of 489DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
RCAS.Sfi c-Akt1
Plasmid#196376Purposecellular Akt1 in avian expression vectorDepositorInsertc-akt1
UseRetroviral; AvianTagsHAExpressionMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
RCAS.Sfi
Plasmid#196377PurposeEmpty avian expression vector RCAS with modifications for fixed orientation cloning into SfiI sitesDepositorTypeEmpty backboneUseRetroviral; AvianTagsExpressionMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Q-ALAS2A-11
Plasmid#166549PurposescFv of a human scaffold targeting 5-aminolevulinate synthase, erythroid-specific, mitochondria. Antigen coverage aa 143 - 587 of 587DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
Q-WNK3B-4
Plasmid#166548PurposescFv of a human scaffold targeting Serine/threonine-protein kinase WNK3. Antigen coverage aa 123-500 of 1800DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
PP-CDPK4 (3DFA)
Plasmid#25127PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertcgd3_920:C29727
UseTagsHisExpressionBacterialMutationKinase domain, residues 70-338PromoterAvailable sinceAug. 5, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
EC71174
Plasmid#154067PurposeLevel 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPERDepositorInsertsHS Cre recombinase
Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
HYGROMYCIN Resistance Cassette
UseCre/Lox and Synthetic Biology; Golden gateTagsExpressionBacterial and PlantMutationThe Arabidopsis thaliana U5 small nuclear ribonuc…Promoter35S promoter, Hordeum vulgare HSP 17 promoter use…Available sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRVV655
Plasmid#119019PurposeDrives TP901-1 recombinase expression in the D. melanogaster germ lineDepositorInsertTP901-1 recombinase
UseTagsSV40 NLSExpressionInsectMutationPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
miR-TDP43b
Plasmid#28212DepositorInsertmiRNAb
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
RCAS.Sfi myr-Akt1
Plasmid#196374PurposeAkt1 with a myristoylation signal in avian expression vectorDepositorInsertmyr-Akt1
UseRetroviral; AvianTagsHA and myristoylationExpressionMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
lvl0 G7t
Plasmid#153380PurposeAgrobacterium terminator for plant expressionDepositorInsertG7 terminator
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCLXSN-GFP
Plasmid#27557DepositorTypeEmpty backboneUseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMX-STAT5A-DN(-351)-Neo
Plasmid#83249PurposeRetroviral expression of STAT5A dominant negative C-terminal deletionDepositorInsertStat5A (Stat5a Mouse)
UseRetroviralTagsExpressionMammalianMutationC-terminal deletion of 351 nucleotides (dominant …PromoterAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
miR-TDP43a
Plasmid#28211DepositorInsertmiRNAa
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only