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Plasmid#131543PurposeExpresses Campylobacter jejuni-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCjCas9
UseCRISPRTagsFLAG-SV40NLS, SV40NLS, and polyAExpressionMammalianMutationHuman codon-optimizedPromoterCAG, T3Available sinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVRc15_524
Plasmid#49736DepositorInsertAS15_524
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpLuxAvailable sinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRa41_491
Plasmid#49702DepositorInsertECF41_491
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRa32_1122
Plasmid#49689DepositorInsertECF32_1122
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin F463A
Plasmid#45975DepositorInsertParkin F463A (Prkn Rat)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromotertacAvailable sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
Py2087ACR1
Plasmid#153032PurposeAnion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).DepositorInsertPy2087ACR1
UseTagsmCherryExpressionMammalianMutationPromoterCMV (+ enhancer)Available sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-SIV-COL-BWC01 vpr
Plasmid#115840PurposeEncodes codon optimized 3xFLAG-SIVCOLVpr (KF214240) and puromycin resistance proteinDepositorInsertSIVCOLVpr (KF214240)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-SIV-RCM-vpr
Plasmid#115838PurposeEncodes codon optimized 3xFLAG-SIVRCM Vpr (HM803689) and puromycin resistance proteinDepositorInsertSIVRCM Vpr (HM803689)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-SIV-SUN-K08 vpr
Plasmid#115832PurposeEncodes codon optimized 3xFLAG-SIVSUN Vpr (FR751162) and puromycin resistance proteinDepositorInsertSIVSUN Vpr (FR751162)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-SIV-GSN-CN166 vpr
Plasmid#115831PurposeEncodes codon optimized 3xFLAG SIVGSN-CN166 Vpr (AF468659) and puromycin resistance proteinDepositorInsertSIVGSN-CN166 Vpr (AF468659)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-SIV-SYK vpr
Plasmid#115821PurposeEncodes codon optimized 3xFLAG-SIVSYK Vpr (L06042) and puromycin resistance proteinDepositorInsertSIVSYK Vpr (L06042)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-SIV-TAL vpr
Plasmid#115820PurposeEncodes codon optimized 3xFLAG-SIVTAL Vpr (AM182197) and puromycin resistance proteinDepositorInsertSIVTAL Vpr (AM182197)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-RlACR_477-EYFP
Plasmid#103771PurposeAnion channelrhodopsin from Rhodomonas lens (RHODO) for expression in mammalian cellsDepositorInsertAnion channelrhodopsin R1ACR_477
UseTagsEYFPExpressionMammalianMutationhuman codon optimizedPromoterCMVAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
SLIRP.HMG6.wt.fl
Plasmid#49821Purposeexpression vector for MBP-SLIRP fusionDepositorInsertSLIRP SRA stem-loop interacting RNA binding protein (SLIRP Human)
UseTagsE. coli Maltose Binding ProteinExpressionBacterialMutationwildtype codon-optimized for E. coli expressionPromoterT7Available sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRc15_436
Plasmid#49735DepositorInsertAS15_436
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpLuxAvailable sinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - Amplicon, BCR/ABL sgRNA 2
Plasmid#70658PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone.DepositorInsertsgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVRa15_524
Plasmid#49668DepositorInsertECF15_524
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-intron-BaLgp160
Plasmid#100918PurposeMammalian expression plasmid for Env from the BaL HIV-1 isolateDepositorInsertHIV-1 (BaL) Env with upstream intron
UseTagsCD5 leader sequenceExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SHIV327C-HuA10-gp160
Plasmid#123270PurposeMammalian expression plasmid for Env from the SHIV327C-Hu A10 strainDepositorInsertSHIV327C-Hu A10 Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-SIV-AGI vpx
Plasmid#115815PurposeEncodes codon optimized 3xFLAG-SIVAGI Vpx (HM803690) and puromycin resistance proteinDepositorInsertSIVAGI Vpx (HM803690)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only