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Plasmid#68257PurposeLevel 0 Golden Gate Part: Promoter and 5UTR Ubiquitin (Zea mays)DepositorInsertPromoter and 5UTR Ubiquitin (Zea mays)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
IY601: pMVP/Lenti/Blast-DEST
Plasmid#121849PurposepMVP destination vector, empty Lentivirus vector backbone w/ Blast selection cassetteDepositorTypeEmpty backboneUseLentiviral; Pmvp gateway destination vectorTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZmUBQ:AVRa1-V1
Plasmid#132357PurposeExpression of the Blumeria graminis f. sp. hordei effector AVRa1-V1 ∆SP from the Zea mays ubiquitin promotorDepositorInsertAVRa1-V1 ∆SP with stop codon
UseTagsExpressionPlantMutationPromoterpZmUBQAvailable sinceOct. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
LC201: pMVP/PB/Puro-DEST
Plasmid#121874PurposepMVP destination vector, empty PiggyBac transposon vector backbone w/ Puro selection cassetteDepositorTypeEmpty backboneUsePiggybac transposon, pmvp gateway destination vec…TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-HEPN
Plasmid#75296PurposeExpresses Sacsin HEPN domain (4441-4579) in fusion with GST (bacterial)DepositorInsertHuman Sacsin 4441-4579
UseTagsGST-TagExpressionBacterialMutationPromoterAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cdk6-DN-HA
Plasmid#1869DepositorInsertCdk6 (CDK6 Human)
UseTagsHAExpressionMammalianMutationDominant negative, D to N in conserved KLAD*FGLAR…PromoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV E2F1 E132
Plasmid#24224PurposeMammalian expression of human E2F1 (DNA binding mutant) with HA tagDepositorInsertE2F1 E132 (E2F1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
Cdk4-DN-HA
Plasmid#1877DepositorInsertCdk4 (CDK4 Human)
UseTagsHAExpressionMammalianMutationDominant negative, D to N in conserved KLAD*FGLAR…PromoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCGN-ELK-1 S383A
Plasmid#27160PurposeFor mammalian expression of human Elk-1 with an HA tag and a mutation in the MAPK phosphorylation site of the transcriptional activation domainDepositorInsertELK1 (ELK1 Human)
UseTagsHAExpressionMammalianMutationTAD mutant: S383APromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetO-Nanog
Plasmid#40800DepositorInsertNanog (Nanog Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterMinimal CMV + TetOAvailable sinceOct. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
DIP2A_pCSdest
Plasmid#53885DepositorInsertDIP2A (DIP2A Human)
UseTagsExpressionMammalianMutationPromoterSP6 and CMVAvailable sinceSept. 8, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
DT40
Plasmid#80415PurposeExpresses 40 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeatsDepositorInserthuman DMPK exons 11-15 with 40 interrupted CTG repeats (DMPK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pG23A
Plasmid#102884PurposePlasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.DepositorInsertCdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)
UseTagsAID(71-114) and mCherryExpressionYeastMutationPromoterNative promoterAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Flag hMcl-1(S159A)
Plasmid#25372DepositorInserthMCL1(S159A) (MCL1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationchanged serine 159 to alaninePromoterAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJK-caCas9-NatMX-Neut5L
Plasmid#89576PurposecaCas9 integrating vector into the Neut5L locusDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-DIO-SN
Plasmid#181741PurposeAAV vector for expressing SNDepositorInsertSuperNova (monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation)
UseAAVTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-mCherry-Rab5
Plasmid#174455PurposeExpress mCherry with early endosome-targeting sequence in mammalian cellsDepositorInsertmCherry with early endosome-targeting sequence
UseTagssmall GTPase (Rab5)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
KX002: pMVP/PB-DEST
Plasmid#121869PurposepMVP destination vector, empty PiggyBac transposon vector backboneDepositorTypeEmpty backboneUsePiggybac transposon, pmvp gateway destination vec…TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
HUNK_pCSdest
Plasmid#53809DepositorInsertHUNK (HUNK Human)
UseTagsExpressionMammalianMutationPromoterSP6 and CMVAvailable sinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pZmUBQ:AVRa1
Plasmid#132356PurposeExpression of the Blumeria graminis f. sp. hordei effector AVRa1 ∆SP from the Zea mays ubiquitin promotorDepositorInsertAVRa1 ∆SP with stop codon
UseTagsExpressionPlantMutationPromoterpZmUBQAvailable sinceOct. 24, 2019AvailabilityAcademic Institutions and Nonprofits only