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Plasmid#105793PurposeExpression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 8885998, 8805248). EGFP is not truly monomeric (PMID: 11988576, 22869113)DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-EGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only
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pKK-TEV-mCherry
Plasmid#105796PurposeExpression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 15558047). mCherry descends from the first truly monomeric red FP mRFP1.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mCherryExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pInducer-TAZ-S89A
Plasmid#213588PurposeDoxycycline inducible expression of TAZ-S89A cDNA in mammalian cellsDepositorInsertTAZ (WWTR1 Human)
UseLentiviralTags2xHAExpressionMammalianMutationS89APromoterAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTE4497
Plasmid#80339Purposemammalian expression FnCpf1 nuclease and Fn crRNADepositorInsertsFn crRNA
FnCpf1
UseCRISPRTags3xHA and NLSExpressionMammalianMutationPromoterCMV and human U6Available sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKK-mRuby3-TEV
Plasmid#105780PurposeExpression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 26879144). mRuby3 is brighter than mCherry and has shifted ex and em spectra.DepositorTypeEmpty backboneUseFlp-in competentTagsmRuby3-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFL His-p97
Plasmid#113510PurposeExpression of His-p97 in insect cells (Baculovirus system)DepositorInsertp97 (VCP Human)
UseTags6*HisExpressionInsectMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pInducer-YAP1-S127A
Plasmid#213584PurposeDoxycycline inducible expression of YAP1-S127A cDNA in mammalian cellsDepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsHAExpressionMammalianMutationS127APromoterAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2(WT)-Fc(IgA1)
Plasmid#145154PurposeMammalian expression plasmid for soluble ACE2-Fc fusion (IgA1)DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsFc region of human IgA1ExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-Ataxin3-Strep-HA
Plasmid#113496PurposeExpression of human Ataxin-3 with C-terminal strep-HA tagDepositorInsertAtaxin-3 (ATXN3 Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas9-D3A
Plasmid#78256PurposeExpresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) under the control of the CMV promoterDepositorInsertDNMT3A CD aa 598-912 (DNMT3A Human)
UseCRISPRTagsFlag epitope tagExpressionMammalianMutationPromoterpCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-BirA-FLAG
Plasmid#105786PurposeExpression of your protein of interest in fusion with BirA-FLAG at the C-terminus (cleavable by TEV). Search for protein partners using the BioID (PMID: 15707685, 22412018).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-BirA-FLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-T131D
Plasmid#213600PurposeExpresses human SKP1 T131D mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-T131D (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mRuby3
Plasmid#105797PurposeExpression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 26879144). mRuby3 is brighter than mCherry and has shifted ex and em spectra.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mRuby3ExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-ELK1
Plasmid#192918PurposeBarcoded piggybac transposon vector with Dox-inducible expression of ELK1DepositorInsertELK1 (ELK1 Human)
UseTagsHAExpressionMammalianMutationPromoterTET3G-miniCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-YBX1
Plasmid#192904PurposeBarcoded piggybac transposon vector with Dox-inducible expression of YBX1DepositorInsertYBX1 (YBX1 Human)
UseTagsHAExpressionMammalianMutationPromoterTET3G-miniCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMIG-hNFATc2/C - AVITEV
Plasmid#74058Purposeretroviral expression plasmid for human NFATc2/C with C-terminal BirA-biotinylation signal and TEV celavage siteDepositorInserthuman NFATc2, isoform C (NFATC2 Human)
UseRetroviralTagsAVI-TEVExpressionMammalianMutationsilent mutation A1723C in the sequence of NM_1730…PromoterpMSCV-LTRsAvailable sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTE4889
Plasmid#88905PurposeExpresses FnCpf1 crRNA and inactive/dead, humanized FnCpf1 nucleaseDepositorInsertsFn crRNA
hFnCpf1(D917A)
UseCRISPRTags3xHA and NLSExpressionMammalianMutationPromoterCMV and human U6Available sinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKK-EGFP-TEV
Plasmid#105776PurposeExpression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 8885998, 8805248). EGFP is not truly monomeric (PMID: 11988576, 22869113)DepositorTypeEmpty backboneUseFlp-in competentTagsEGFP-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2-WT(732)
Plasmid#154098PurposeMammalian expression plasmid for dimeric soluble ACE2 (untagged)DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsExpressionMammalianMutationExtracellular, soluble protease and neck domains …PromoterCMVAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-FOSB
Plasmid#192901PurposeBarcoded piggybac transposon vector with Dox-inducible expression of FOSBDepositorInsertFOSB (FOSB Human)
UseTagsHAExpressionMammalianMutationPromoterTET3G-miniCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only