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Plasmid#161824PurposeMammalian expression plasmid for RBD of bat coronavirus isolate Rs4231DepositorInsertReceptor-binding domain (RBD) of spike protein S
UseTagsGSG linker and 8his tag and HA leader sequenceExpressionMammalianMutationHuman codon optimized RBD (a.a. T320-D518)PromoterCMVAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-hTRAF3 1-448
Plasmid#44033DepositorInsertTNF receptor-associated factor 3 (TRAF3 Human)
UseTags1xHAExpressionMammalianMutationContains amino acids 1-448; V274D and S393GPromoterAvailable sinceApril 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CHRFAM7A-mGFP
Plasmid#62633Purposemammalian expression of human duplicated Alpha7 (CHRFAM7A) with mGFP fused into M3-M4 loopDepositorInsertCHRFAM7A (CHRFAM7A Human)
UseTagsExpressionMammalianMutationmGFP inserted in M3-M4 loopPromoterCMVAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
mcherry Gamma 9 ---pcDNA3.1
Plasmid#64203PurposeG protein Gamma cone(9) subunit tagged with mcherry on the N terminusDepositorInsertG protein Gamma 9 (GNGT2 Human)
UseTags6xHis and mcherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-LKB1(KD)
Plasmid#84640PurposemCherry-tagged, LKB1(K78I); kinase-dead mutantDepositorInsertmCherry-LBK1(K78I) (STK11 Synthetic, Human)
UseTags6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …ExpressionMammalianMutationLysine 78 mutated to Isoleucine; kinase deadPromoterCMVAvailable sinceMay 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Prom1 SV8
Plasmid#184011PurposeMammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286).DepositorInsertProm1 (Prom1 Mouse)
UseTagsFlagExpressionMammalianMutationWild type cDNAPromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_RGG-GFP-RGG_withTEVsite
Plasmid#124939PurposeExpresion of RGG_GFP-RGGDepositorInsertRGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsGFPExpressionMammalianMutationTEV site between RGG and EGFPPromoterAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag Jnk1a1MKK7B2
Plasmid#19731DepositorUseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-dCas9-CibN
Plasmid#60552PurposeExpresses dCas9-CibN in mammalian cellsDepositorInsertdCas9-CibN
UseCRISPRTagsFLAG and SV40 NLSExpressionMammalianMutationinactivating Cas9 mutations D10A and H840APromoterCMVAvailable sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1his p66shc S36A
Plasmid#10973DepositorInsertp66shc (SHC1 Human)
UseTagsHisExpressionMammalianMutationS36APromoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Akt-STOPS
Plasmid#175006PurposeAkt Substrate-based Tandem Occupancy Peptide Sponge. Genetically encoded for perturbing cellular Akt kinase activity.DepositorInsertmCherry-Akt-STOPS
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-PNGase-Strep-HA
Plasmid#113490PurposeExpression of human PNGase with C-terminal strep-HA tagDepositorInsertPNGase (NGLY1 Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CARMA2sh-E138A
Plasmid#97068PurposeExpresses Psoriasis-Linked mutant CARD14/CARMA2 in mammalian cellsDepositorInsertCARD14 isoform 3 (CARD14 Human)
UseTagsHAExpressionMammalianMutationchanged Glutamic Acid 138 to AlaninePromoterCMVAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RAB-AKARev(T/A)
Plasmid#118479PurposeNegative-control mutant for RAB-AKARev biosensor.DepositorInsertRAB-AKARev(T/A)
UseTags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable sinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO HSPH1
Plasmid#19453DepositorInsertHSPH1 (HSPH1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
S100B_pcDNA6.2/EmGFP-Bsd
Plasmid#176952PurposeMammalian expression vector encoding S100B and EmGFP-BsdDepositorInsertS100B (S100B Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK2 K235R
Plasmid#80872Purposemammalian expression of ALK2 K235RDepositorInsertALK2 (ACVR1 Human)
UseTagsHAExpressionMammalianMutationK235RPromoterCMVAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG_FBXL7 (R310H)
Plasmid#171849PurposepcDNA3-FLAG_FBXL7 (R310H)DepositorInsertF-box and leucine rich repeat protein 7 (FBXL7 Human)
UseTagsFlagExpressionMammalianMutationchanged Arginine 310 to HistidinePromoterAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1(-) TRIM12B
Plasmid#87079PurposeMurine homologues of human Trim5DepositorInsertTRIM12B (Trim5 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-N-3XFLAG-Axin1
Plasmid#123463DepositorInsertAxin 1 (Axin1 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only