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Plasmid#171953PurposeExpression vector for ecDHFR-asLOV2 fusion protein. Also has TYMSDepositorInsertFusion of ecDHFR and asLOV2 at position 121 of DHFR
UseTags8-His tagExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1C-YFP-shSCR
Plasmid#139647PurposeReplacing the RSV promoter of pLKO.1 with the CMV promoter improves lentiviral production with third-generation viral packaging system.DepositorInsertshSCR
UseLentiviral and RNAiTagsExpressionMammalianMutationSee Depositor commentsPromoterCMVAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBL-2
Plasmid#162769PurposeMinimal backbone with AmpRDepositorTypeEmpty backboneUseSynthetic Biology; Molecular cloningTagsExpressionMutationPromoternoAvailable sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_DL121_WTTS
Plasmid#171954PurposeSelection vector for chimeric fusion of ecDHFR and asLOV2.DepositorInsertsFusion of ecDHFR and asLOV2 at position 121 of DHFR
Thymidylate Synthase
UseTags8-His tagExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM862
Plasmid#169085PurposeMoClo level 0 BvDODA1 coding sequence moduleDepositorInsertBvDODA1
UseSynthetic Biology; Moclo cloning vectorTagsExpressionMutationPromoterAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM1021
Plasmid#169084PurposeMoClo level 0 BvCYP76AD1 coding sequence moduleDepositorInsertBvCYP76AD1
UseSynthetic Biology; Moclo cloning vectorTagsExpressionMutationPromoterAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 ΔH1 WD
Plasmid#163580Purposeexpress Dcp2 ΔH1 WD at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationW50A, D54A, L255APromoterDCP2 promoterAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 ΔH1
Plasmid#163576Purposeexpress Dcp2 ΔH1 (L255A) at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationLysine at position 255 is mutated to AlaninePromoterDCP2 promoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 VAC8-G2A-GFP
Plasmid#166837PurposeExpresses a myristoylation mutant Vac8-GFP in yeasts, mutation is Gly2AlaDepositorInsertVAC8 (VAC8 Budding Yeast)
UseTagsGFPExpressionYeastMutationG2APromoterVAC8 promoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVG97
Plasmid#164849PurposePlasmid for replacement of PEST sequence in strains with pr-yEVenus-PEST-ADH1t constructDepositorInsertPart of URA3 5' UTR and yEVenus with stop codon for recombination with pr-yEVenus-PEST-ADH1t:URA
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 300
Plasmid#163577Purposeexpress Dcp2 300 at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationTruncation of C-terminal domian (301-970)PromoterDCP2 promoterAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBEL1647
Plasmid#155148PurposeExpresses MlaF 1-246, taglessDepositorInsertmlaF(1-246)
UseTagsExpressionMutationPromoteraraBADAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1840
Plasmid#155150PurposeExpresses MlaF-6xHisDepositorInsertMlaF-6xHis
UseTagsExpressionMutationPromoterT7 promoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1244
Plasmid#155138PurposeExpresses MlaFED operon in E. coliDepositorInsertmlaFE-[6xHis-2xQH-TEV-mlaD]-mlaC operon
UseTagsExpressionMutationPromoteraraBADAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1245
Plasmid#155139PurposeExpresses MlaF in E. coliDepositorInsert6xHis-TEV-mlaF
UseTagsExpressionMutationPromoterT7 promoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1246
Plasmid#155141PurposeExpresses MlaB in E. coliDepositorInsert6xHis-TEV-mlaB
UseTagsExpressionMutationPromoterT7 promoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1266
Plasmid#155142PurposeExpresses MlaF, tagless, araBAD promoterDepositorInsertMlaF
UseTagsExpressionMutationPromoteraraBADAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-MBP_F
Plasmid#146318PurposeMammalian Expression of MBPDepositorInsertMBP (MBP Human)
UseTagsExpressionMammalianMutationN217SPromoterAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG502-MAP4-frFAST
Plasmid#160058PurposeExpresses MAP4-frFAST in mammalian cellsDepositorInsertMAP4-frFAST
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-1050R (Ubiq-dCasRx)
Plasmid#132417PurposeExpress dCasRx under Ubiquitin-63E promoterDepositorInsertdCasRx
UseTagsExpressionInsectMutationPromoterUbiqAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only