Skip to main content
Addgene
Showing: 2601 - 2620 of 16483 results

  1. pMBEC8

    Plasmid
    #187599
    Purpose
    Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Apramycin resistance
    Depositor
    Pablo Ivan Nikel
    Article
    Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:
    Insert
    nCas9-BEC-UGI; cas6f, gfp
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    trc
    Available since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    187599-plasmid-map-sequence-id-367572

    Enlarge

  4. pMuLE ENTR CMV loxP L5-L2

    Plasmid
    #62094
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and multiple cloning site flanked by loxP sites. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    Cre/Lox; Mule gateway entry vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62094-plasmid-map-sequence-id-101383

    Enlarge

  8. pSLiP-G1G2

    Plasmid
    #188965
    Purpose
    L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown
    Depositor
    Seung-Goo Lee
    Article
    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131
    Inserts
    dCas9
    sgRNA 1: atcggtcgcattgttttccactagg, sgRNA 2: gttagacgctgattacatggactagg
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PrhaBAD
    Available since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    188965-plasmid-map-sequence-id-374348

    Enlarge

  9. pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::B1-NLSLacZ-B2

    Plasmid
    #186413
    Purpose
    Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined NLSLacZ under control of an AttB3/B5 recombined reg. sequence.
    Depositor
    Patrick Lemaire
    Article
    Roure et al PLoS One. 2007. 2(9):e916.
    Insert
    lacZ (lacZ E. coli)
    Use
    Gateway destination vector
    Tags
    Nuclear localization signal
    Expression
    Mutation
    Promoter
    Available since
    Aug. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    186413-plasmid-map-sequence-id-369044

    Enlarge

  10. pTK-EdnrbE2-AVI-Tfap2B-tev-FLAG-2A-Citrine

    Plasmid
    #127776
    Purpose
    Modified pTK plasmid containing chicken EdnrB E2 enhancer driving expression of full length Chicken Tfap2B in frame with an Avi tag at the N terminus and FLAG-tag and Citrine at the C-terminus
    Depositor
    Tatjana Sauka-Spengler
    Article
    Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517
    Insert
    tfap2b (TFAP2B Chicken)
    Use
    Unspecified
    Tags
    Avi
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    127776-plasmid-map-sequence-id-250021

    Enlarge

  11. pSB1C3_mTq-Tat (c-plasmid)

    Plasmid
    #127635
    Purpose
    Encodes a fluorecent protein with an RNA binding peptide: mTurquoise2-tat.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme PlmJ and RBS BBa_B0034.
    Depositor
    Friedrich Simmel
    Article
    Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.
    Insert
    mTurquoise2
    Use
    Synthetic Biology
    Tags
    RNA binding peptide: PCP and RNA binding peptide:…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    127635-plasmid-map-sequence-id-263753

    Enlarge

  12. CoxVIIIx2-CeNL(Ca2+)_19μ-pcDNA3

    Plasmid
    #111923
    Purpose
    Cyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_19μ
    Depositor
    Takeharu Nagai
    Article
    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/
    Insert
    CeNL(Ca2+)_19μ
    Use
    Tags
    CoxVIIIx2
    Expression
    Mammalian
    Mutation
    E67D, F92W, E104D, D133E at CaM
    Promoter
    CMV
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    111923-plasmid-map-sequence-id-222573

    Enlarge

  13. CoxVIIIx2-CeNL(Ca2+)_1.2μ-pcDNA3

    Plasmid
    #111922
    Purpose
    Cyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_1.2μ
    Depositor
    Takeharu Nagai
    Article
    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/
    Insert
    CeNL(Ca2+)_1.2μ
    Use
    Tags
    CoxVIIIx2
    Expression
    Mammalian
    Mutation
    E67D, E104D, D133E at CaM
    Promoter
    CMV
    Available since
    June 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    111922-plasmid-map-sequence-id-214013

    Enlarge

  14. pG3H-PE3max-attR1R2

    Plasmid
    #213049
    Purpose
    Destination vector Expressing nCas9 and RT fusion protein and carrying Gateway recombination sites
    Depositor
    Bing Yang
    Article
    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1
    Inserts
    Cas9 nickase
    M-MLV Reverse Transcriptase
    Use
    Note: this plasmid needs helper pvs1-vir2 for rep…
    Tags
    Expression
    Plant
    Mutation
    H840A, R240K, N413K,
    Promoter
    Available since
    Feb. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    213049-plasmid-map-sequence-id-422145

    Enlarge

  16. RGB-S reporter

    Plasmid
    #207841
    Purpose
    A three-colour stress biosensor for real time analysis of physiological stress, genotoxicity, and cytotoxicity of Escherichia coli
    Depositor
    Christof Niemeyer
    Article
    Zoheir et al NPJ Biofilms Microbiomes. 2023 Aug 21;9(
    Inserts
    RpoH sensing construct
    SOS sensing construct
    RpoS sensing construct
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PosmY, PsulA, and grpE
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    207841-plasmid-map-sequence-id-426759

    Enlarge

Showing: 2601 - 2620 of 16483 results