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We narrowed to 35,149 results for: lat

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pCAG Cas9-2A-Citrine

Plasmid

#92358

Purpose

CAG-driven ubiquitous expression of Cas9. Contains 2A-Citrine reporter. For CRISPR mediated gene knockouts in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

Cas9 2A Citrine

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-TSC2-NLS

Plasmid

#192441

Purpose

Encodes mCherry-tagged wild-type TSC2 isoform 5 targeted to the nucleus.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

mCherry-TSC2-NLS (TSC2 Human)

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pUCHR-osTIR1-IRES-GFP

Plasmid

#110661

Purpose

Lentiviral vector for stable expression of osTIR1 and selection with GFP

Depositor

Dmitriy Mazurov

Article

Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

Insert

osTIR1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

March 1, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-Cdk9 T186A

Plasmid

#28101

Depositor

Andrew Rice

Article

Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

Insert

Cdk9 (CDK9 Human)

Use

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

Contains a T186A mutation in Cdk9

Promoter

Available since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/NS3

Plasmid

#120822

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

NS3 ZIKV

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/NS2B

Plasmid

#120819

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

NS2B ZIKV

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCIG-V5-Snail2-IRES-nls-GFP

Plasmid

#44282

Depositor

Martin Cheung

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Feb 19;11

Insert

V5-Snail2 (SNAI2 Chicken)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV-IE-enhancer and chicken beta-actin promoter

Available since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pCDH_TNF-SBP-EGFP

Plasmid

#65282

Purpose

TNF reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Use

Lentiviral

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mutation

Promoter

CMV

Available since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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pFLEX-m-cyclin D1 -T286A mutant

Plasmid

#86792

Purpose

mammalian expression of cyclin D1 T286A mutant

Depositor

Charles Sherr, Martine Roussel

Article

Matsushime et al Cell. 1991 May 17;65(4):701-13.

Insert

cyclin D1 (Ccnd1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

T286A

Promoter

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-ELL pBacPAK8

Plasmid

#15322

Depositor

Joan Conaway, Ronald Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

eleven nineteen lysine rich in leukemia (ELL Human)

Use

Baculovirus construction

Tags

FLAG

Expression

Mutation

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-MTS-WT-c-Src-FLAG

Plasmid

#44652

Depositor

Yoshimi Homma

Article

Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

Insert

v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)

Use

Tags

FLAG tag and mitochondria-targeting sequence (res…

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

April 26, 2013

Availability

Academic Institutions and Nonprofits only

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ATF4 6: mCD36.GFP

Plasmid

#21853

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

mCD36 (Cd36 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Only the first 13 AA of CD36 are fused to GFP

Promoter

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pJW6.01

Plasmid

#62882

Purpose

Transgene plasmid for expressing GFP::MEG-3 under meg-3 promoter and 3'UTR

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

GFP::MEG-3

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

meg-3

Available since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1hSIRT4_HA

Plasmid

#24482

Depositor

Eric Verdin

Article

Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

Insert

Sirtuin 4 (SIRT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 23, 2010

Availability

Academic Institutions and Nonprofits only

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pXD70CT5-PxylR-CymR, Pveg(CuO)-LacZ

Plasmid

#191627

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5 (engineered from pCT5-bac2.0)-LacZ

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

lacZ

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSilencer2.1-U6-3JB1F+2

Plasmid

#190833

Purpose

For mammalian expression of 3JB1F+2, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.

Depositor

Gerald Hart

Article

Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

Insert

3JB1F_T1+2bp (A Dual-Specificity aptamer targeting OGT and GFP)

Use

Affinity Reagent/ Antibody and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pPBO.ACT005

Plasmid

#182715

Purpose

Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region

Depositor

Joseph Schoeniger

Article

Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

Insert

CasRx crRNA cloning backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Catalytically deactivated R295A, H300A, R849A, H8…

Promoter

pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

Available since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pQHA-USP7 CS puroR

Plasmid

#46754

Purpose

Retroviral vector that expresses catalytically inactive form of HA-tagged human USP7

Depositor

Gordon Peters, Goedele Maertens

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP7 (USP7 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

C223S--catalytically inactive

Promoter

CMV

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pHsp83-NLS-HA-2xPCP-2xGFP

Plasmid

#71243

Purpose

Used to label reporter mRNAs. Drosophila expression of the PCP protein fused to EGFP.

Depositor

Anne Ephrussi

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

2xPCP-2xGFP

Use

P element-mediated germline transformation

Tags

EGFP

Expression

Insect

Mutation

Promoter

Hsp83

Available since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pIS1-Eef25UTR-TOPmut-renilla

Plasmid

#38236

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Eef2_TOPm_5UTR/Renilla (Eef2 Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

The first nucleotides of the 5' UTR were cha…

Promoter

Endogenous Eef2

Available since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only