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Plasmid#214761PurposeXylose reductase from Hypocrea jecorina fused with GST-tagged and codon optimized for expression in E. coliDepositorInsertglutathione S-transferases fused with xylose reductase
UseTagsGlutathione S-transferasesExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-BNT162b2ĪUTR
Plasmid#171214PurposeGateway entry vector containing the BNT162b2/tozinameran cDNA sequence lacking the original 5' and 3' UTRs and the poly(A) tail. The original Kozak sequence has also been altered.DepositorInsertBNT162b2ĪUTR
UseGateway cloningTagsExpressionMutationKozak sequence changed from CCCGCCACC to GGCTGCACā¦PromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pcDNATM3.1/V5-His TOPO-PKCi-A120E
Plasmid#55687Purposeexpresses constitutively active human PKCi tagged with V5DepositorInsertHomo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
UseTagsV5/HisExpressionMammalianMutationA120EPromoterCMVAvailable sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprt
Plasmid#92166PurposePlasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vectorDepositorInsertQuinolinate Phosphoribosyltransferase (QPRT Human)
UseTagsTEV protease -cleavable N-terminal 6HisExpressionBacterialMutationchanged Asp 2 to Asn to create BspHI sitePromoterT7Available sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTK165_Mem-mCherry-4.1G-CTD
Plasmid#46362DepositorInsert4.1G C-terminal domain (EPB41L2 Human)
UseTagsMem (N-terminal 20 amino acids of neuromodulin foā¦ExpressionMammalianMutationC-terminal domain from 886-1005 amino acidsPromoterCMVAvailable sinceAug. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
PERK 5: PERK.K618R.pGEX4T-1
Plasmid#21818DepositorInsertPERK (537-1114) K618R (Eif2ak3 Mouse)
UseTagsGST and Myc (9E10)ExpressionBacterialMutationPERK Kinase Domain. Amino acids 537-1114. Kinase ā¦PromoterAvailable sinceMarch 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-KD-mAbl
Plasmid#69028PurposeRetorviral vector encoding Kinase Defective mouse c-AblDepositorInsertmouse c-Abl Type VI - kinase defective
UseRetroviralTagsFLAG tag inserted at Internal SalI site (2934 bp)ExpressionMammalianMutationThe KD (kinase defective) mutation is at Lysine 2ā¦PromoterAvailable sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
As0
Plasmid#123158PurposeArsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ109, ParsR-ABS67, PhrpLE, PrinAAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT/TO Plk4 KD ā24-mCherry
Plasmid#80272Purposemammalian expression plasmid for kinase dead Plk4 with multi-phospho degron deletedDepositorInsertPlk4 (PLK4 Human)
UseTagsmCherryExpressionMammalianMutationdeletion of multi-phospho degron (amino acids 282ā¦PromoterCMVAvailable sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
XE120 CAMKII-K42M 1-271-CS2+
Plasmid#16734DepositorInsertCAMKII-K42M 1-271 (Camk2a Rat)
UseXenopus expressionTagsExpressionMutationC-terminal truncation of K42M kinasePromoterAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pX330.puro sgRNA KGA Stop1
Plasmid#110405PurposeCRISPR/Cas9 vector with sgRNA for glutaminase KGA isoform C-Terminal Knock-in and puromycin resistanceDepositorInsertGLS glutaminase (GLS Human)
UseCRISPRTagsExpressionMammalianMutationPromoterCBhAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
GSX2-P2A-tGFP-DTA
Plasmid#161750PurposetGFP plasmid for the c-terminal targeting of human GSX2 locusDepositorInsertturboGFP
UseCRISPRTagsP2A-tGFPExpressionMammalianMutationPromoterendogenous GSX2 promoterAvailable sinceDec. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACEBAC1-CoREST-C
Plasmid#109159PurposeEncodes a human C-terminal CoREST fragment with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman C-terminal CoREST, sequence-optimized for insect cells (RCOR1 Human)
UseTags3xFLAGExpressionInsectMutationPromoterPolyhedrinAvailable sinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLA CMV N-Flag RALB V23 R47
Plasmid#50983PurposeExpresses RALB-V23/R47 in mammalian cellsDepositorInsertRALB (RALB Human)
UseLentiviralTagsFlagExpressionMammalianMutationG23V, K47RPromoterCMVAvailable sinceFeb. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLA CMV N-Flag RALB V23 R160
Plasmid#50982PurposeExpresses RALB-V23/R160 in mammalian cellsDepositorInsertRALB (RALB Human)
UseLentiviralTagsFlagExpressionMammalianMutationG23V K160RPromoterCMVAvailable sinceFeb. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGY577
Plasmid#166663PurposeLight-inducible Cre recombinase (LiCre) lentiviral expression plasmid for light-induced Cre/Lox recombinationDepositorInsertLiCre
UseCre/Lox and LentiviralTagsExpressionMutationCre: E340A, D341APromoterCMVAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C3-PLK4 K41M-3xFLAG
Plasmid#69838PurposeThis plasmid encodes kinase dead PLK4 isoform 1 (K41M mutation) with an N-terminal EGFP tag and C-terminal 3xFLAG tagDepositorInsertPLK4 (PLK4 Human)
UseTags3xFLAG tag and EGFPExpressionMammalianMutationK41MPromoterCMVAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mitoExRai-AKAR2
Plasmid#161757PurposeExRai-AKAR2 targeted to the outer mitochondrial membrane.DepositorInsertDAKAP-ExRai-AKAR2
UseTagsN-terminal 30 amino acids from DAKAP1ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGV15 (CK2alpha K68M)
Plasmid#27089DepositorInsertCK2alpha K68M (kinase dead) (CSNK2A1 Human)
UseTagsHAExpressionMammalianMutationK68M (kinase dead)PromoterCMVAvailable sinceMay 17, 2012AvailabilityAcademic Institutions and Nonprofits only