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Plasmid#120439PurposeBarcoded lentiviral vector to express FOXA2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertFOXA2 (FOXA2 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-NCOA7g1 (BB22)
Plasmid#139457PurposeLentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable markerDepositorInsertNCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-NCOA7g2 (BB23)
Plasmid#139458PurposeLentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable markerDepositorInsertNCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC22A8
Plasmid#131989PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC22A8 (SLC22A8 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR-53BP1 1-1972 28A
Plasmid#53447PurposeRecombinational donor/master vectorDepositorInsert53BP1 (TP53BP1 Human)
UseGatewayTagsExpressionMutationI914T mutation; All 28 SQ/TQ motifs in N-term of …PromoterAvailable sinceDec. 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSpG-PPE
Plasmid#170130PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertnCas9-SpG(H840A)-M-MLV
UseCRISPRTagsExpressionPlantMutationH840A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T1…Promotermaize Ubiquitin-1Available sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-IRES-BSD
Plasmid#209751PurposeLentiviral transfer plasmid to express the blasticidin resistance geneDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BRD9-PGK-Puro-IRES-GFP
Plasmid#75114PurposeRetroviral expression plasmid encoding wild type human BRD9DepositorInsertBRD9 (BRD9 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Flrt2-Fc-His
Plasmid#72074PurposeExpresses the extracellular region of the FLRT2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertFlrt2 (Flrt2 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 shETV5-B
Plasmid#74978PurposeshETV5 shRNADepositorInsertETV5 (ETV5 Human)
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.5 shETV5-A
Plasmid#74977PurposeshETV5 shRNADepositorInsertETV5 (ETV5 Human)
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-hSOX17
Plasmid#206389PurposeExpresses human SOX17 in mammalian cells, for lentivirus generation.DepositorInsertSOX17 (SOX17 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
CHRM2-DuET
Plasmid#213208PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCHRM2 (CHRM2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCI-Myc3-HPS4
Plasmid#133931PurposeExpresses 3xMyc-HPS4 construct in mammalian cellsDepositorInsertHPS4 (HPS4 Human)
UseTags3xMyc tagExpressionMammalianMutationGlutamic acid 229 to Glycine; Valine 552 to Methi…PromoterCMV I.E.Available sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Mouse Alpha2 delta-2 HA pMT2
Plasmid#58731Purposeexpression of HA tagged mouse Alpha2 delta-2 in mamalian cellsDepositorInsertalpha2 delta-2 (Cacna2d2 Mouse)
UseTagsExpressionMammalianMutationPromoterSV40Available sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TRE-FH-TET3 short
Plasmid#166916PurposeLentivirus for inducible overexpression of human TET3 short isoform lacking CXXC domain (NM_001366022.1)DepositorInsertTET3 short isoform
UseLentiviralTagsHA TagExpressionMammalianMutationChanged nucleotide 18 (G) to (A) to delete FseI r…PromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMVp-dCas9-3xNLS-VP64 (Construct 1)
Plasmid#55195PurposeExpresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector.DepositorInsertdCas9
UseCRISPR and Synthetic BiologyTagsVP64ExpressionMammalianMutationPromoterCMV/hUBCAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS2-GFP-MKLP1
Plasmid#140566PurposeExpresses GFP tagged MLKP1DepositorInsertMKLP1 (KIF23 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMV IE94Available sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF-Bos TRAM Flag
Plasmid#41551DepositorInsertTRAM (TICAM2 Human)
UseTags6xHis and FlagExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBZCas13b-HEPN
Plasmid#89901PurposeBacterial expression for bzCas13b and crRNA with both HEPN domains mutated. New spacers can be cloned by digesting with BsaI.DepositorInsertCas13b
UseTagsExpressionBacterialMutationR116A/H121A/R1177A/H1182APromoterLacAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only