Addgene

We narrowed to 5,016 results for: hwa

Showing: 2581 - 2600 of 5016 results

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p4xSMAD_RE

Plasmid

#118051

Purpose

GBPart - Operator/DNA Response element - 4 copies the SMAD DNA binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

4 copies the SMAD DNA binding motif

Use

Synthetic Biology; Domestication of dna parts for…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4D

Plasmid

#52521

Purpose

Co-expresses HA tagged channelrhodopsin and hM4D from a Cre-dependent virus

Depositor

Scott Sternson

Article

Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

Insert

ChR2-2a-hM4D

Use

AAV and Cre/Lox

Tags

2xHA

Expression

Mutation

Promoter

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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CABE-T1.17

Plasmid

#193260

Purpose

Expression of CABE-T1.17 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CABE-T1.17

Use

Tags

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1

Plasmid

#98972

Purpose

Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 1 and Cas9(D10A)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV5 MEKK4alpha WT

Plasmid

#12187

Depositor

Gary Johnson

Article

Gerwins et al J Biol Chem. 1997 Mar 28. 272(13):8288-9

Insert

MEKK4 (Map3k4 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
IKK2-EYFP

Plasmid

#111196

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Birbach et al J Biol Chem. 2002 Mar 29;277(13):10842-5

Insert

IKKbeta (IKBKB Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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THOC5

Plasmid

#156084

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

THOC5 (THOC5 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pRW004-SM-destination-proUBQ10-Cas9

Plasmid

#104438

Purpose

Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;

Depositor

Detlef Weigel

Article

Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial and Plant

Mutation

Promoter

UBQ10

Available since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNAIIIB rhoA QL

Plasmid

#61632

Purpose

mammalian expression of rhoA constitutively active mutant

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

rhoA (RHOA Human)

Use

Tags

Expression

Mammalian

Mutation

Q63L, constitutively active

Promoter

CMV

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pBeast-pAhpC-RBS- LacZ

Plasmid

#190060

Purpose

LacZ containing colorimetric reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pAhpC promoter

Depositor

Jerome Bonnet

Article

Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

Insert

lacZ

Use

Synthetic Biology; Cell-free protein synthesis

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pEF005-sgRNA-shuffle-in

Plasmid

#104441

Purpose

Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF004.

Depositor

Detlef Weigel

Article

Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

Insert

sgRNA-transRNA transcription by U6 promoter

Use

CRISPR

Tags

Expression

Bacterial and Plant

Mutation

Promoter

Available since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pEF004-sgRNA-shuffle-in

Plasmid

#104440

Purpose

Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF005

Depositor

Detlef Weigel

Article

Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

Insert

sgRNA-transRNA transcription by U6 promoter

Use

CRISPR

Tags

Expression

Bacterial and Plant

Mutation

Promoter

U6 promoter

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pTACR TH1-p2aGFP

Plasmid

#135815

Purpose

CRISPR based on px330 p2aGFP targeting TH gene near stop codon

Depositor

Tim Ahfeldt, Lee Rubin

Article

Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

Insert

TH 1 guide

Use

CRISPR

Tags

p2a Myr-eGFP

Expression

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pTACR TH2-p2aGFP

Plasmid

#135816

Purpose

CRISPR based on px330 p2aGFP targeting TH gene near stop codon

Depositor

Tim Ahfeldt, Lee Rubin

Article

Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

Insert

TH 2 guide

Use

CRISPR

Tags

p2a Myr-eGFP

Expression

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/HIS/FLAG/HA-PES1

Plasmid

#38085

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

PES1 pescadillo ribosomal biogenesis factor 1 (PES1 Human)

Use

Tags

HIS/FLAG/HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-mEos3.1 (mEosFP3.1-A69T)

Plasmid

#99221

Purpose

Bacterial expression of the primed conversion capable protein pr-mEos3.1 (mEos3.1-A69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos3.1

Use

Tags

Expression

Bacterial

Mutation

A69T for primed conversion applications

Promoter

T7

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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Lck-mCherry-RGS

Plasmid

#112935

Purpose

Plasmid encodes a membrane located inhibitor of Ga12/13 signaling (RGS domain from p115)

Depositor

Dorus Gadella

Article

Reinhard et al Mol Biol Cell. 2017 Nov 7;28(23):3371-33

Insert

p115RhoGEF-RGS domain

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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1 kb Axin2 basic

Plasmid

#25701

Depositor

Eric Fearon

Article

Leung et al J Biol Chem. 2002 Jun 14. 277(24):21657-

Insert

Axin 2 promoter (AXIN2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pcDNAIIIB HA JNK

Plasmid

#61630

Purpose

mammalian expression of JNK

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

JNK1 beta1 isoform (MAPK8 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pHIS3p:yomRuby3-Tub1+3'UTR::LEU2

Plasmid

#204288

Purpose

yeast codon optimized mRuby3 alpha-tubulin plasmid for integration into the endogenous TUB1 ORF

Depositor

Linda Huang

Article

Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

Insert

pHIS3p:yomRuby3-Tub1+3'UTR::LEU2

Use

Tags

mRuby3

Expression

Yeast

Mutation

Promoter

pHis3

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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