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Plasmid#80077PurposeRetroviral expression of mouse EZH2 Y641F mutant, hygromycin selectionDepositorInsertsUseRetroviralTags5' FLAGExpressionMammalianMutationY641F, R342R, N393NPromoterAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only
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pFLAG-CMV4-ZIKV-NS4A
Plasmid#178845PurposeMammalian expression of ZIKV ORFDepositorInsertZIKV-NS4A
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-CMV4-ZIKV-2KNS4B
Plasmid#178847PurposeMammalian expression of ZIKV ORFDepositorInsertZIKV-2KNS4B
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV14-3X-Flag-SARS-CoV-2 S
Plasmid#145780PurposeOver expression of SARS-CoV-2 S (spike) in mammalian cell lineDepositorInsertSARS-CoV-2 S (S SARS CoV 2)
UseTags3xFlagExpressionMammalianMutationCodon optimized and C-terminal 19 amino acids del…PromoterCMVAvailable sinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFLAG-CMV-hErk1(K71R)
Plasmid#49329PurposeExpresses kinase deficient FLAG-tagged human Erk1DepositorInsertErk1 (MAPK3 Human)
UseTagsFLAGExpressionMammalianMutationK71RPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMma PylT_FLAG-Mma PylRS AF
Plasmid#140023PurposePlasmid with 4xMmaPylT cassette and MmaPylRS AF for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
UseTagsFLAGExpressionMammalianMutationY271A, Y349FPromoterEF1Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-OTUB1 C91S-pcDNA3.1
Plasmid#118210PurposeExpresses FLAG-OTUB1 C91S catalytic inactive mutant in mammalian cellsDepositorInsertOTUB1 (OTUB1 Human)
UseTags3x FLAGExpressionMammalianMutationchanged cysteine 91 to serinePromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
hMLKL NB(1-182)-2xFv-flag
Plasmid#106075Purposeexpression in mammalian cellsDepositorInserthuman MLKL NB(1-182)-2xFv-flag (MLKL Human)
UseRetroviral; Tet-on retrovirusTagsflagExpressionMammalianMutationamino acids 1-182PromoterDox-inducibleAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-GFP
Plasmid#175757PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-GFP (Control for APEX experiments)DepositorInsertGFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
FLAG-OTUB1 T134R-pcDNA3.1
Plasmid#118211PurposeExpresses FLAG-OTUB1 T134R mutant in mammalian cellsDepositorInsertOTUB1 (OTUB1 Human)
UseTags3x FLAGExpressionMammalianMutationchanged threonine 134 to argininePromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG CMV14 / BRAF-D594N
Plasmid#131713PurposeMammalian Expression of Flag tagged BRAFDepositorInsertBRAF (BRAF Human)
UseTags3xFLAGExpressionMammalianMutationD594NPromoterCMVAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/HIS/FLAG/HA-MYBBP1A
Plasmid#38084DepositorInsertMYBBP1A MYB binding protein (P160) 1a (MYBBP1A Human)
UseTagsHIS/FLAG/HAExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPROEX-HTc-Flag3-Nrf2
Plasmid#21553DepositorInsertnuclear factor (erythroid-derived 2)-like 2 (NFE2L2 Human)
UseTags6xHis and Flag3ExpressionBacterialMutationPromoterAvailable sinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-FLAG-hSF3B1-K700E
Plasmid#82577PurposeExpresses a codon-optimized ORF of human SF3B1 ( K700E mutant)DepositorInserthuman SF3B1-K700E (SF3B1 Human)
UseTagsFLAG peptideExpressionMammalianMutationK700EPromoterCMVAvailable sinceMarch 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-FLAG-SHLD2
Plasmid#114117PurposeExpresses N-terminally tagged FLAG-SHLD2 in mammalian cellsDepositorInsertSHLD2 (SHLD2 Human)
UseGenomic integration, tetracycline inducibleTagsFLAGExpressionMammalianMutationPromoterCMV/TetO2Available sinceAug. 28, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA flag PPAR gamma
Plasmid#8895PurposeMammalian expression of flag-tagged PPAR gammaDepositorInsertPPAR gamma (Pparg Mouse)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FLAG-GFP-Tmem192
Plasmid#134630Purposelysosomal markerDepositorInsertTMEM192 (TMEM192 Human)
UseLentiviralTagsFLAG and GFPExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-pro-Il1b-C-Flag
Plasmid#75131PurposeExpresses C-terminal flag-tagged pro-IL-1β in mammalian cellsDepositorInsertinterleukin 1 beta (Il1b Mouse)
UseTags3XFLAGExpressionMammalianMutation(please see depositor comments below)PromoterCMVAvailable sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) Flag-His-ATM kd
Plasmid#31986DepositorInsertATM kd (ATM Human)
UseTagsFlag and HisExpressionMammalianMutation3'UTRf 9 nucl/D2870A N2875k. catalytically …PromoterAvailable sinceMarch 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pACYC-FLAG-dN6-His
Plasmid#22143DepositorInsertFlag-clpXdeltaNLinkedHexamer-His6
UseTagsFlag, His6, and L20 LinkersExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only