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Plasmid#178530PurposeExpresses Lifeact-miRFP703 in mammalian cellsDepositorInsertLifeact-miRFP703
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2-CAG-fGFP
Plasmid#108714PurposeTransposon donor plasmid for long-term expression of membrane targetted GFPDepositorInsertEGFP
UseTags6xHis and farnesylation signalExpressionMammalianMutationPromoterCAGAvailable sinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJ204-loxP-100RPU.2-coEPO-BGHpA-cHS4-100RPU.2-coEPO-lox2272
Plasmid#154829PurposeDonor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing two copies of erythropoietinDepositorInsertscoEPO
coEPO
UseCre/LoxTagsExpressionMammalianMutationCodon-optimized (Cricetulus griseus)Promoter100RPU.2Available sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mEGFP-loxP-P2A-dTKneo-pA-loxP
Plasmid#139484PurposePCR template for mEGFP gene knock-in donor vectorDepositorInsertmEGFP-loxP-P2A-dTKneo-pA-loxP
UseTagsmEGFPExpressionMammalianMutationPromoterCAG promoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_CIC_iso1
Plasmid#135728PurposeDonor vector for 3' FLAG tag of human CIC_iso1DepositorInsertCIC_iso1 Homology arms (CIC Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
ppk301-T2A-QF2 HDR plasmid
Plasmid#130667PurposePlasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti ppk301 geneDepositorInsertppk301-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; ppk301-right-HDR-arm
UseDonor templateTagsExpressionMutationPromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMC_mNG2(11)_BSDminus_F0
Plasmid#184162PurposeDonor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.DepositorInsertsmNG2(11)
bsd
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
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CMV-mEGFP-PKCg-C1
Plasmid#112266PurposeITRACKgamma Donor ConstructDepositorInsertprotein kinase c gamma (Prkcg Mouse)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCPP3416
Plasmid#128720PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopT1-1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLS.546
Plasmid#184963PurposeTest effect of a1/a2 length on editing priB using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 22
UseTagsExpressionBacterialMutationpriB donor G1069A, a1/a2 length extended to 22 bpPromoterT7/lacAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC-STITCH
Plasmid#129535PurposeSTITCH (Synthetic Toplogical Insulator with TetO for CHromatin-engineering) with homology arms for MYC locus insertion.DepositorInsertSTITCH
UseCRISPR; Donor templateTagsExpressionMutationPromoterAvailable sinceDec. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5108
Plasmid#128726PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopAA1-2
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5107
Plasmid#128725PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopAA1-1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pCPP3373
Plasmid#128718PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopQ1-1
UseTagsExpressionBacterialMutationSynonymous point mutation in the wobble position…PromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMC_mNG2(11)_BSDminus_F2
Plasmid#184164PurposeDonor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.DepositorInsertsmNG2(11)
bsd
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC_mNG2(11)_BSDminus_F1
Plasmid#184163PurposeDonor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.DepositorInsertsmNG2(11)
bsd
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.540
Plasmid#184959PurposeTest effect of a1/a2 length on editing fabH using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 22
UseTagsExpressionBacterialMutationfabH donor G954A, a1/a2 length extended to 22 bpPromoterT7/lacAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEM-myr-eGFP-SV40pA-FKF
Plasmid#119866PurposeDonor cassette containing myr-tagged eGFP and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertmyr-tagged eGFP and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
UseTags2x myristoylationExpressionBacterialMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only