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Plasmid#169984PurposeTet/Dox-inducible mammalian expression of CARD8 that cannot bind DPP9 due to a mutation at site I (E274R) with sgRNA binding site mutationsDepositorInsertCARD8 (CARD8 Human)
UseLentiviralTagsHAExpressionMammalianMutationPAM, E274RPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND F405G + S297A
Plasmid#169974PurposepDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F405G)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationS297A, F405GPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND E274R + S297A
Plasmid#169968PurposepDONR221 for Gateway shuttling. Contains the CARD8 autoprocessing-deficient FIIND (L162M-P434, no stop codon) that cannot bind DPP9 at site I (E274R)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationS297A, E274RPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND R394E + S297A
Plasmid#169972PurposepDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (R394E)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationS297A, R394EPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 PAM E274R
Plasmid#169977PurposepDONR221 for Gateway shuttling. Contains CARD8 that cannot bind DPP9 due to a mutation at site I (E274R) with sgRNA binding site mutationsDepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationPAM, E274RPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A CARD8-FLAG R394E + S297A
Plasmid#169955PurposeMammalian expression of CARD8-FLAG with a site III mutation (R394E) with an additional autoprocessing mutation (S297A)DepositorInsertCARD8 (CARD8 Human)
UseTagsFLAGExpressionMammalianMutationS297A, R394EPromoterCMVAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A CARD8-FLAG F405G + S297A
Plasmid#169957PurposeMammalian expression of CARD8-FLAG with a site III mutation (F405G) with an additional autoprocessing mutation (S297A)DepositorInsertCARD8 (CARD8 Human)
UseTagsFLAGExpressionMammalianMutationS297A, F405GPromoterCMVAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A CARD8-FLAG E274R + S297A
Plasmid#169951PurposeMammalian expression of CARD8-FLAG that cannot bind DPP9 due to a mutation at site I (E274R) with an additional autoprocessing mutation (S297A)DepositorInsertCARD8 (CARD8 Human)
UseTagsFLAGExpressionMammalianMutationE274R, S297APromoterCMVAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQC hKGA.S286A V5-His IRES G418
Plasmid#110391Purposeγ-Retroviral transfer vector for expressing glutminase isoform KGA (S286A), C-terminal V5-6xHis tags, IRES-driven Geneticin selection.DepositorInsertKGA glutaminase (GLS Human)
UseRetroviralTags6xHis and V5ExpressionMammalianMutationS286APromoterCMVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
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CYTIP_PDZ_1
Plasmid#103930PurposeProtein expression and purification of PSCDBP PDZ domainDepositorInsertCYTIP (CYTIP Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CD2AP-3/3
Plasmid#91508PurposeProtein expression and purification of human SH3 domain construct CD2AP-3/3DepositorInsertCD2AP-3/3 (CD2AP Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ARHGEF38-1/1
Plasmid#91373PurposeProtein expression and purification of human SH3 domain construct ARHGEF38-1/1DepositorInsertARHGEF38-1/1 (ARHGEF38 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
MYO3B gRNA (BRDN0001145051)
Plasmid#75568Purpose3rd generation lentiviral gRNA plasmid targeting human MYO3BDepositorInsertMYO3B (MYO3B Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MYO3B gRNA (BRDN0001145615)
Plasmid#75570Purpose3rd generation lentiviral gRNA plasmid targeting human MYO3BDepositorInsertMYO3B (MYO3B Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MYO3B gRNA (BRDN0001147808)
Plasmid#75571Purpose3rd generation lentiviral gRNA plasmid targeting human MYO3BDepositorInsertMYO3B (MYO3B Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MYO3B gRNA (BRDN0001145445)
Plasmid#75569Purpose3rd generation lentiviral gRNA plasmid targeting human MYO3BDepositorInsertMYO3B (MYO3B Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-ET-F656W
Plasmid#72546Purposeexpresses FLAG tagged human BRD4 ET domain (608-699 aa) with F656W mutationDepositorInsertBRD4 ET (BRD4 Human)
UseTagsFLAGExpressionMammalianMutationF656WPromoterAvailable sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only