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Plasmid#125703PurposeYeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selectionDepositorInsertymNeongreen
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPL5628_TEF1*-Cre_MET15
Plasmid#60933PurposeConstitutive expression vector for Cre-recombinase containing MET15 selection marker.DepositorInsertCre
UseTagsDriven from a mutant TEF1 promoter with 0.16x act…ExpressionYeastMutationPromotermutant TEF1Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-ymScarletI-CaURA3
Plasmid#118457PurposeYeast tagging vector to create a gene fusion with yeast optimized mScarletI with CaUra3 selectionDepositorInsertymScarletI
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPL5608_TEF1*-Cre_TRP1
Plasmid#60931PurposeConstitutive expression vector for Cre-recombinase containing TRP1 selection marker.DepositorInsertCre
UseTagsDriven from a mutant TEF1 promoter with 0.16x act…ExpressionYeastMutationPromoterMutant TEF1Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJYDC3
Plasmid#162460PurposeYeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter DnbALFA was fused to the N-terminus of Aga2p.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsDnbALFA designed ALFA nanobody, HA tag, HDEL sequ…ExpressionMutationPromoterAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJYDN3
Plasmid#162456PurposeYeast display platform plasmid N3 which allows for simultaneous expression of the protein of interest at the N-terminus of Aga2p yeast agglutinin and eUnaG2 or nbALFA detection.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsALFA tag, HA, myc tags, and eUnaG2 fluorescent pr…ExpressionMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJYDN2
Plasmid#162454PurposeYeast display plasmid intended for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA) and expression of N-terminal fusions with Aga2p yeast agglutinin.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsDnbALFA nanobody and Ha and mycExpressionMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
HO-pGAL-poly-KanMX4-HO
Plasmid#51664Purposeyeast plasmid for integration of target sequence at HO locus, Kan selection, pGAL inducible promoterDepositorTypeEmpty backboneUseYeast integrationTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTPGI_dCas9
Plasmid#64279Purposeencodes yeast-optimized dCas9 synthetic transcription factorDepositorInsertYeast-optimized dCas9
UseTagsSV40 NLSExpressionYeastMutationD10A & H840A relative to S. pyogenes Cas9PromoterpTPGIAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-ymTurquoise2-CaURA3
Plasmid#118458PurposeYeast tagging vector to create a gene fusion with yeast optimized mTq2 with CaUra3 selectionDepositorInsertymTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPL5606_TEF1*-Cre_ADE2
Plasmid#60932PurposeConstitutive expression vector for Cre-recombinase containing ADE2 selection marker.DepositorInsertCre
UseTagsDriven from a mutant TEF1 promoter with 0.16x act…ExpressionYeastMutationPromotermutant TEF1Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-ytdTomato-CaURA3
Plasmid#118461PurposeYeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selectionDepositorInsertytdTomato
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-ymVenus-CaURA3
Plasmid#118459PurposeYeast tagging vector to create a gene fusion with yeast optimized mVenus with CaUra3 selectionDepositorInsertymVenus
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZCAN
Plasmid#129115PurposeZ. rouxii centromeric vector containing ClonNAT resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-A1-FLAG:hphMX4
Plasmid#134429PurposePCR-based C-terminal A1-FLAG tagDepositorInsertA1-FLAG
UsePcr-based tagging in yeastTagsExpressionMutationPromoterAvailable sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pZEN
Plasmid#129113PurposeZ. rouxii episomal vector containing ClonNAT resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-A1-FLAG:natMX4
Plasmid#134432PurposePCR-based C-terminal A1-FLAG tagDepositorInsertA1-FLAG
UsePcr-based tagging in yeastTagsExpressionMutationPromoterAvailable sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-S6-FLAG:kanMX6
Plasmid#134438PurposePCR-based C-terminal S6-FLAG tagDepositorInsertS6-FLAG
UsePcr-based tagging in yeastTagsExpressionMutationPromoterAvailable sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-SRS-FLAG:hphMX4
Plasmid#134445PurposePCR-based C-terminal SRS-FLAG tagDepositorInsertSRS-FLAG
UsePcr-based tagging in yeastTagsExpressionMutationPromoterAvailable sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAG424-ccdB
Plasmid#70124PurposeYeast Gateway TRP1 2micron vector, no promoter (GPD1 promoter removed)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterNoneAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only