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Plasmid#38035DepositorInsertshp53 (TP53 Human)
UseRNAiTagsExpressionMutationPromoterAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-CRAF_S259A
Plasmid#51127Purposemammalian expression of wt CRAF S259ADepositorInsertCRAF (RAF1 Human)
UseRetroviralTagsExpressionMammalianMutationS259APromoterAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-mOrange2
Plasmid#96938PurposeRetroviral plasmid containing mOrange2 for labeling of cellsDepositorInsertMonomeric Orange2
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
ST-SBP-EGFP_puromycin
Plasmid#65270PurposeST reporter only ( no hook) (RUSH system)DepositorInserttruncated sialyltransferase (ST6GAL1 Human)
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationcontains only amino acids 1 to 43 of STPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-AuroraAKD-C-7
Plasmid#54010PurposeLocalization: Centrosomes, Excitation: 487, Emission: 509DepositorInsertAuroraA
UseTagsmEmeraldExpressionMammalianMutationKinase Dead - I57V and K162RPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBABEpuro-ARAF_S214C
Plasmid#51077Purposemammalian expression of ARAF S214CDepositorInsertARAF (ARAF Human)
UseRetroviralTagsExpressionMammalianMutationS214CPromoterAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-CRAF_S257L
Plasmid#51125Purposemammalian expression of wt CRAF S257LDepositorInsertCRAF (RAF1 Human)
UseRetroviralTagsExpressionMammalianMutationS257LPromoterAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pINT-PuroDDEYFP
Plasmid#31442DepositorInsertenhanced yellow fluorescent protein
UsePhic31/attbTagsFKBP L106PExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSUPER.retro.puro-shMePCE_shLARP7
Plasmid#113538PurposeRetroviral vector designed to knock down MePCE and LARP7 simultaneously.DepositorInsertshMePCE and shLARP7 (MEPCE Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-IRESMuro
Plasmid#82336PurposeBase vector targeting genes to the GAPDH locus of human cells. Inserted genes are expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.DepositorInsertIRESMuro
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGCGFP-puro
Plasmid#31266DepositorInsertGCGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pISH-Neurod1
Plasmid#74396PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Neurod1. Not for gene expression.DepositorInsertneurogenic differentiation 1 (Neurod1 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-Haunt E1-PuroT2AmCherry-4A KI
Plasmid#92164PurposeFor lncRNA 4_pA(3_SV40 polyA and 1 BGH polyA) homologue recombination mediated knockinDepositorInsertHaunt P-Inr2-cDNA KI homology arms
UseOtherTagsExpressionMutationPromoterAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Puro_LPAR_AKAR-WT
Plasmid#125206PurposeSB-transposon plasmid for stable expression of lipid raft-targeted FRET-based PKA activity reporterDepositorInsertLPAR_AKAR-WT
UseTransposonTagsEV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…ExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-mtagtdTom_IRESMuro
Plasmid#82355PurposeVector targeting the mTagtandem tomato gene to the GAPDH locus of human cells. It is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.DepositorInsertmtagTandemTomato-IRESMuro
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pISH-Neurog1
Plasmid#74395PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Neurog1. Not for gene expression.DepositorInsertneurogenin 1 (Neurog1 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro KR ATG3
Plasmid#26927DepositorInsertautophagy-related 3 (yeast) (Atg3 Mouse)
UseRetroviralTagsHis and V5ExpressionMammalianMutationChanged Lysine 243 to Arginine.PromoterAvailable sinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLKO.puro_shHuR 3UTR
Plasmid#110414PurposeTRCN0000276186 (Target TTGTTAGTGTACAACTCATTT), silence human ELAVL1 (HuR) gene, puromycin selectionDepositorInsertELAVL1 (HuR)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 (RNA PolIII)Available sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
33-G-X1-pEF1-puro
Plasmid#111604PurposeExpress human 33-G-X1 in 293T cellsDepositorInserthuman LRRC33 and GARP chimeria (NRROS Human)
UseTagsFlag tagExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDESTFLAGHApuro3-Ago2K533AK570A
Plasmid#14104DepositorInsertAgo2 (AGO2 Human)
UseTagsFLAG/HAExpressionMammalianMutationchanged Lys533 to Ala, Lys570 to AlaninePromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only