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Plasmid#83537PurposeConstitutive TEF1 promoter mediated expression of the UBiC, ARO4*, and TKL1 genesDepositorInsertpTEF1-UBiC-CYC1t-pTEF1-ARO4*-CYC1t-pTEF1-TKL1-CYC1t
UseTagsExpressionYeastMutationPromoterpTEF1Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX317 TEAD2
Plasmid#193686PurposeConstitutive lentiviral expression of TEAD2DepositorInsertTEAD2 (TEAD2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCOA-Leu-FvPPT1-fsr1
Plasmid#179868PurposeConstitutive expression vector for Saccharomyces cerevisiae comprising the polyketide synthase fsr1 from Fusarium vanettenii and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1DepositorInsertsPPT1
fsr1
UseTagsExpressionYeastMutationCodon optimized for expression in Saccharomyces c…PromoterYarrowia lipolytica EXP1 and Yarrowia lipolytica …Available sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGG431
Plasmid#165615PurposeVector for expression of SpCas9 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-CYC1t (KanR cassette in place of the PID)DepositorInsertTEF1p-SpCas9::KanR(in place of PID)-1xNLS-CYC1t (S. cerevisiae TEF promoter driving SpCas9 with KanR cassette replacing PID)
UseCRISPRTagsSV40 NLSExpressionYeastMutationCorrected homology relative to wild type SpCas9 (…PromoterTEF1Available sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-TEAD4-IDR
Plasmid#166451PurposeExpresses fusion of mCherry and TEAD4-IDRDepositorInsertmCherry, TEAD4-IDR(89-230) (TEAD4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF2693
Plasmid#142359PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTEAD4 (TEAD4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneoLuc-TEAD4
Plasmid#128335PurposeVector for LUMIER assayDepositorInsertTEAD4 (TEAD4 Human)
UseTagsLuciferaseExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
Pet22b-mCherry-TEAD4-IDR
Plasmid#166442PurposeBacterial expression of 6x His tagged mCherry-TEAD4 -IDR fusion proteinDepositorInsertmCherry, TEAD4-IDR (TEAD4 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-TEAD4-YBD
Plasmid#166450PurposeExpresses fusion of mCherry and TEAD4-YAP binding domainDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-Cry2-mCherry-TEAD4-IDR
Plasmid#166449PurposeExpresses fusion of CRY2PHR with mCherry and TEAD4-IDRDepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
PHBA02
Plasmid#83536PurposeConstitutive TEF1 promoter mediated expression of the UBiC and ARO4* genesDepositorInsertpTEF1-ARO4*-CYC1t-pTEF1-UBiC-CYC1t
UseTagsExpressionYeastMutationPromoterpTEF1Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJT1
Plasmid#98299PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoterDepositorInsertPTEF2-ERG20-TRPL3-PTEF1-AcNES1-TRPL41B
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJT2
Plasmid#98306PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoterDepositorInsertPTEF2>ScERG20N127W>TRPL3>PTEF1>AcNES1>TRPL41B
UseTagsExpressionYeastMutationERG20 N127WPromoterAvailable sinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIRHD-TetR-GAL80-Rho1-GAL4
Plasmid#218274PurposeIntroduction of low-temperature-inducible GAL system, LowTempGAL v2.2DepositorInsertgal80Arm1-pAgTEF1>KanMX4>tAgTEF1-pHAC1>UBI4>>DHFRP66L-TetR>NLS>TUP1>tABF1-pTEF2>Rho1C17R>>GAL4>tGAL4-pTEF1(TetO)>GAL80Arm2
UseTagsExpressionYeastMutationDHFR (P66L), Rho1 (C17R)PromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTXB3-Flag PLK1
Plasmid#68271Purposebacterial expression of Plk1DepositorInsertPLK1 (PLK1 Human)
UseTagsFlag and Mxe intein/chitin binding domainExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTXB3-Flag PLK1 T210D
Plasmid#68272Purposebacterial expression of Plk1 T210D mutantDepositorInsertPLK1 (PLK1 Human)
UseTagsFlag and Mxe intein/chitin binding domainExpressionBacterialMutationT210DPromoterT7Available sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS426GFP-2×PH(PLCδ)
Plasmid#36092DepositorInsert2xPH(PLCdelta) fusion
UseTagsGFPExpressionYeastMutationcontains aa11-140 of PLCδ (including the PH domai…PromoterPRC1Available sinceJune 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRS424GFP-2xPH(Osh2)
Plasmid#36095DepositorInsert2xPH(Osh2) fusion
UseTagsGFPExpressionYeastMutationPromoterPRC1Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a-nGFPMNase
Plasmid#187826Purposeexpress nGFPMNase protein in E coliDepositorInsertnGFPMNASE
UseTagshis tagExpressionBacterialMutationPromoterT7 PromotorAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP1F5
Plasmid#165064PurposeCharacterisation of TEF1 promoter using yEGFP as reporter gene and PGK1 terminatorDepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1> (BamHI)yEGFP>T-PGK1-T-URA3
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only