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We narrowed to 1,083 results for: pgem

Showing: 241 - 260 of 1083 results

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pGEMHE-mScarlet-MAP4-MTBD

Plasmid

#171488

Purpose

T7 promotor drives in vitro transcription of mScarlet-tagged mouse MAP4-Microtubule Binding Domain mRNA

Depositor

Melina Schuh

Article

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

MAP4-MTBD (Map4 Mouse)

Use

Pgehme

Tags

mScarlet

Expression

Mutation

Aminoacids 658-1126

Promoter

T7

Available since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

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pGem-PgpdA-Cre-TtrpC

Plasmid

#168784

Purpose

Helper vector for the transient expression of Cre recombinase in filamentous fungi. Not replicative in fungi.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102

Insert

Cre

Use

Cre/Lox and Synthetic Biology; Expression in fila…

Tags

Expression

Mutation

Promoter

PgpdA

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pGEMHE-miRFP-MAP4-MTBD

Plasmid

#171487

Purpose

T7 promotor drives in vitro transcription of miRFP-tagged mouse MAP4-Microtubule Binding Domain mRNA

Depositor

Melina Schuh

Article

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

MAP4-MTBD (Map4 Mouse)

Use

Pgehme

Tags

miRFP

Expression

Mutation

Aminoacids 658-1126

Promoter

T7

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mCherry-mTrim21ΔRING-Box

Plasmid

#105523

Purpose

T7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted RING-box mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mCherry

Expression

Mutation

deletion of RING domain (aa124-462)

Promoter

T7

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pGEMT-Map7-1333-end

Plasmid

#46070

Depositor

Edgar Gomes

Article

Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

Insert

Map7-1333-end (Map7 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mEGFP-mTrim21ΔRING-Box

Plasmid

#105520

Purpose

T7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted RING-box mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mEGFP

Expression

Mutation

deletion of RING domain (aa124-462)

Promoter

T7

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pGEM-and1 in situ probe

Plasmid

#52102

Purpose

actinodin 1 and1 antisense in situ probe linearize w/ ApaI SP6 pol

Depositor

Didier Stainier

Article

Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:

Insert

actinodin 1 in situ probe (and1 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pGEM mouse PEA3 [TJ#200]

Plasmid

#16346

Depositor

Thomas Jessell

Insert

PEA3 (Etv4 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 4, 2009

Availability

Academic Institutions and Nonprofits only
pGEM-sgMUC4_dual-MTS-3x-Set I

Plasmid

#162762

Purpose

Separately expressing 3 unique sgMUC4_dual-MTS (SetI)

Depositor

Antony K Chen

Article

Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

Insert

sgMUC4_dual-MTS_1; sgMUC4_dual-MTS_2; sgMUC4_dual-MTS_3

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pGEM-T Easy-Gbait-hs-QF2

Plasmid

#122563

Purpose

Integration of QF2 sequence using the CRISPR/Cas9 system.

Depositor

Marnie Halpern

Article

Choi et al Elife. 2021 Dec 8;10:e72345. doi: 10.755

Insert

Gbait-hsp70-QF2-SV40pA

Use

Cloning

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pGEMT-halo-HA-p2a-neoR

Plasmid

#161977

Purpose

homologous donor template for endogenous tagging

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

GAlinker-HA-halo-HA-p2a-neoR

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMT-puroR-p2a-HA-halo

Plasmid

#161979

Purpose

homologous donor template for endogenous tagging

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

puroR-p2a-HA-halo-GSlinker

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pGEM-T Easy-Gbait-hs-Cre

Plasmid

#122562

Purpose

Integration of Cre sequence using the CRISPR/Cas9 system.

Depositor

Marnie Halpern

Article

Choi et al Elife. 2021 Dec 8;10:e72345. doi: 10.755

Insert

Gbait-hsp70-Cre-SV40pA

Use

Cloning

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pGEM-sgIGR_dual-MTS-3x-Set I

Plasmid

#162764

Purpose

Separately expressing 3 unique sgIGR_dual-MTS (SetI)

Depositor

Antony K Chen

Article

Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

Insert

sgIGR_dual-MTS_1; sgIGR_dual-MTS_2; sgIGR_dual-MTS_3

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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Rab18b cds in pGem-T Easy

Plasmid

#80539

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab18b (rab18b Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Available since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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Rab18a cds in pGem-T Easy

Plasmid

#80538

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab18a (rab18a Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Available since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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pGEMT-Pax7bait-T2a-Cherry-pA

Plasmid

#111154

Purpose

knock in bait for Axolotl Pax7 locus

Depositor

Elly Tanaka

Article

Fei et al Proc Natl Acad Sci U S A. 2017 Nov 21;11

Insert

Pax7bait-T2a-Cherry-pA

Use

Tags

Expression

Mammalian

Mutation

wt

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pGEM-rat FceRI alpha

Plasmid

#8373

Depositor

Jean Kinet

Article

Kinet et al Biochemistry 1987 Jul 28;26(15):4605-10.

Insert

rat Fc epsilon RI alpha cDNA (Fcer1a Rat)

Use

Beta-gal

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 7, 2005

Availability

Academic Institutions and Nonprofits only
pGEMT-aid-p2a-GFP

Plasmid

#161976

Purpose

homologous donor template for endogenous tagging

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

GSlinker-aid-GFP

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMT-GFP-p2a-aid

Plasmid

#161975

Purpose

homologous donor template for endogenous tagging

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

GFP-aid-GSlinker

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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