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Plasmid#172152PurposepylBCD variant in pCDF backboneDepositorInsertPT7/lac (pylBCD 40C_sub-pop6); LacI
UseTagsExpressionBacterialMutationPylB(N61S, E175K, E178K)PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH463
Plasmid#172155PurposepylBCD variant in pBAD backboneDepositorInsertPara (pylBCD 36A_sub-pop1); AraC
UseTagsExpressionBacterialMutationPylB(N61S, E122K, E178K, G188R)PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH573
Plasmid#172157PurposepylBCD variant in pBAD backboneDepositorInsertPara (Untagged pylBCD); AraC
UseTagsExpressionBacterialMutationCodon-optimized Methanosarcina acetivorans pylBCDPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH574
Plasmid#172158PurposepylBCD variant in pBAD backboneDepositorInsertPara (pylBCD 36A_sub-pop2); AraC
UseTagsExpressionBacterialMutationPylB(N61S, E84K, E122K) PylC(E316D)PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH575
Plasmid#172159PurposepylBCD variant in pBAD backboneDepositorInsertPara (pylBCD 34B_sub-pop3); AraC
UseTagsExpressionBacterialMutationSumo(S6L) PylB(N61S, E178K, G188R, E251D) PylD(A2…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH576
Plasmid#172160PurposepylBCD variant in pBAD backboneDepositorInsertPara (pylBCD 34B_sub-pop4); AraC
UseTagsExpressionBacterialMutationPylB(N61S, E175K, E178K, E251D) PylD(A252V)PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH60
Plasmid#172142PurposeMicroscopy inclusion body assayDepositorInsertpCDF.CD
UseTagsExpressionBacterialMutationPylCD partial operon (PylB deleted to assess impa…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH123
Plasmid#172146PurposeMicroscopy inclusion body assayDepositorInsertpCDF.B.(SD4)CD
UseTagsExpressionBacterialMutationPylBCD operon (RBS inserted for PylC)PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH126
Plasmid#172147PurposeMicroscopy inclusion body assayDepositorInsertpCDF.SUMO-B.(SD4).CD
UseTagsExpressionBacterialMutationPylBCD operon (SUMO tag inserted for PylB; RBS in…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS_1N_400
Plasmid#170476PurposeTo amplify transcription template containing LacI operatorsDepositorInsertT7A1, O1, O-, Lambda t1 in lacZ fragment
UseTagsExpressionBacterialMutationPromoterT7A1Available sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC038
Plasmid#156345PurposeExpresses heterologous rRNADepositorInsertrRNA operon, orthogonal-antiRBS
UseSynthetic BiologyTagsExpressionMutationWT antiRBS engineered to orthogonal antiRBS, PS e…PromoterAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRF033d
Plasmid#156354PurposeExpresses heterologous rRNADepositorInsertrRNA operon, orthogonal-antiRBS
UseSynthetic BiologyTagsExpressionMutationWT antiRBS engineered to orthogonal antiRBS, PS e…PromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRF031d
Plasmid#156352PurposeExpresses heterologous rRNADepositorInsertrRNA operon, orthogonal-antiRBS
UseSynthetic BiologyTagsExpressionMutationWT antiRBS engineered to orthogonal antiRBS, PS e…PromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC019
Plasmid#156350PurposeExpresses heterologous rRNADepositorInsertrRNA operon, orthogonal-antiRBS
UseSynthetic BiologyTagsExpressionMutationWT antiRBS engineered to orthogonal antiRBS, PS e…PromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRF034d
Plasmid#156355PurposeExpresses heterologous rRNADepositorInsertrRNA operon, orthogonal-antiRBS
UseSynthetic BiologyTagsExpressionMutationWT antiRBS engineered to orthogonal antiRBS, PS e…PromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRF028d
Plasmid#156349PurposeExpresses heterologous rRNADepositorInsertrRNA operon, orthogonal-antiRBS
UseSynthetic BiologyTagsExpressionMutationWT antiRBS engineered to orthogonal antiRBS, PS e…PromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRF035d
Plasmid#156356PurposeExpresses heterologous rRNADepositorInsertrRNA operon, orthogonal-antiRBS
UseSynthetic BiologyTagsExpressionMutationWT antiRBS engineered to orthogonal antiRBS, PS e…PromoterAvailable sinceDec. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRF030d
Plasmid#156351PurposeExpresses heterologous rRNADepositorInsertrRNA operon, orthogonal-antiRBS
UseSynthetic BiologyTagsExpressionMutationWT antiRBS engineered to orthogonal antiRBS, PS e…PromoterAvailable sinceDec. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC041
Plasmid#156348PurposeExpresses heterologous rRNADepositorInsertrRNA operon, orthogonal-antiRBS
UseSynthetic BiologyTagsExpressionMutationWT antiRBS engineered to orthogonal antiRBS, PS e…PromoterAvailable sinceDec. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC040
Plasmid#156346PurposeExpresses heterologous rRNADepositorInsertrRNA operon, orthogonal-antiRBS
UseSynthetic BiologyTagsExpressionMutationWT antiRBS engineered to orthogonal antiRBS, PS e…PromoterAvailable sinceDec. 29, 2020AvailabilityAcademic Institutions and Nonprofits only