-
Plasmid#229212PurposeCRISPR array plasmid for NOTCH2 (Spacers 1-24)DepositorArticleInsertCRISPR array plasmid for NOTCH2 (Spacers 1-24)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCSCherryN5icd
Plasmid#22456DepositorInsertNotch3 (notch3 Zebrafish)
UseExpression vectorTagsmCherryExpressionMutationPromoterAvailable sinceDec. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
453 pCSMTN5ICD
Plasmid#22466DepositorInsertNotch3 (notch3 Zebrafish)
UseExpression vectorTagsExpressionMutationPromoterAvailable sinceSept. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_IRES_mC_pGK_tBFP
Plasmid#79123PurposeLentiviral vector for inducible expression of genes with Gal4DBDVP64 synNotch ReceptorsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_pGK_mCherry
Plasmid#79124PurposeLentiviral vector for inducible expression of genes with Gal4DBDVP64 synNotch Receptors (no IRES)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_tBFP_PGK_mCherry
Plasmid#79130PurposeLentiviral vector - tBFP reporter for Gal4DBDVP64 synNotch receptors with a constitutive mCherryDepositorInserttBFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAG-RbpjEnR
Plasmid#160924Purposedominant-negative construct to inhibit Notch signalingDepositorInsertRbpjEnR
UseTagsExpressionMutationfused with an engrailed repressorPromoterAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG-EnRRbpj
Plasmid#160925Purposedominant-negative construct to inhibit Notch signalingDepositorInsertEnRRbpj
UseTagsExpressionMutationfused with an engrailed repressorPromoterAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
625 pTol2-tp1bglob:egfp
Plasmid#73586PurposeTol2 vector with Notch responsive tp1 element driving expression of EGFPDepositorInserttp1 element driving EGFP
UseTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
502 p5E-tp1
Plasmid#73585Purposeentry vector (L4-R1) containing the Notch responsive tp1 element upstream of a basal promoterDepositorInserttp1 element
UseTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR-NICD1
Plasmid#46048DepositorInsertNotch1 (C-term) (Notch1 Mouse)
UseEntry vectorTagsMyc tagExpressionMutationCytoplasmic domain initiating at amino acid 1753PromoterAvailable sinceJuly 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
p160Tol2-her4.3:switchCas9
Plasmid#227680PurposeExpression of cre inducible and her4.3 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs for SABER-seq in zebrafishDepositorInsertsloxP-dsRed-loxP-Cas9-t2A-GFP
4xU6:sgRNA
UseCRISPRTagsExpressionMutationPromoterU6 and her4.3Available sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
p104Tol2-tp1:Cas9-t2A-GFP, 4xU6:sgRNA
Plasmid#227773PurposeExpression of tp1 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs in zebrafishDepositorInsertsCas9-t2A-GFP
4xU6:sgRNA
UseCRISPRTagsExpressionMutationPromoterU6 and tp1Available sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1a-NICD1
Plasmid#46047DepositorInsertNotch1 (C-term) (Notch1 Mouse)
UseTagsMyc tagExpressionMammalianMutationCytoplasmic domain initiating at amino acid 1753PromoterEF1aAvailable sinceJuly 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1aH NICD1
Plasmid#86500PurposeMammalian expression of Myc tagged NICD1DepositorInsertNotch1 (C-term) (Notch1 Mouse)
UseTagsMycExpressionMammalianMutationCytoplasmic domain only (AA 1753-2531)PromoterEF1aAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato
Plasmid#113865PurposeTriple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation.DepositorInsertsCMV-eGFP
TOP-iRFP
PTCH1-mTurquoise2
CBF-tdTomato
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_EXPR_CMV-eGFP_TOP-NLuc1.1_12GLI-FLuc_CBF-GLuc
Plasmid#113862PurposeTriple pathway reporter, 3P-Luc; wnt-NLuc1.1, hedgehog-FLuc, notch-GLuc; plus CMV-eGFP. Gateway expression vector for lentivirus generation.DepositorInsertsCMV-eGFP
TOP-NLuc1.1
12GLI-Fluc
CBF-GLuc
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLPC-JAG1-mVenus
Plasmid#171170PurposeRetroviral vector for CMV promoter driven expression of JAG1 (Notch pathway) fused to mVenus fluorescent protein (to be used in conjunction with Phoenix packaging cells).DepositorInsertJAG1 (JAG1 Human)
UseRetroviralTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLPC-dnMAML1-mVenus
Plasmid#171023PurposeRetroviral vector for CMV promoter driven expression of dominant negative (DN) MAML1 (Notch pathway) fused to mVenus fluorescent protein (to be used in conjunction with Phoenix packaging cells).DepositorInsertMAML1 Dominant Negative (MAML1 Human)
UseRetroviralTagsmVenusExpressionMammalianMutationamino acids 12-74 only, hence conferring dominant…PromoterCMVAvailable sinceApril 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCLE
Plasmid#17703DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 12, 2008AvailabilityAcademic Institutions and Nonprofits only