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Plasmid#225352PurposeLentiviral vector for BFP expressionDepositorInsertBFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDF-His_TEV_MsbA
Plasmid#191476PurposeExpresses MsbA with a TEV protease cleavable N terminal His TagDepositorInsertMsbA
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL CusS NarX Chimera
Plasmid#225652PurposeExpresses a NarX-based chimera capable of sensing copper (II). For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertCusS-NarX
UseCell free expressionTagsMycExpressionBacterialMutationTransmembrane and sensing domain replaced to dete…PromoterT7Available sinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NrsS NarX Chimera
Plasmid#225653PurposeExpresses a NarX-based chimera capable of sensing nickel (II). For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertNrsS-NarX
UseCell free expressionTagsMycExpressionBacterialMutationTransmembrane and sensing domain replaced to dete…PromoterT7Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL RssA NarX Chimera
Plasmid#225654PurposeExpresses a NarX-based chimera capable of sensing iron (III). For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertRssA-NarX
UseCell free expressionTagsMycExpressionBacterialMutationTransmembrane and sensing domain replaced to dete…PromoterT7Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NarX Myc
Plasmid#225651PurposeExpresses WT NarX capable of sensing nitrate. For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertNarX (narX E. coli)
UseCell free expressionTagsMycExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL VanS NarX Chimera
Plasmid#225655PurposeExpresses a NarX-based chimera capable of sensing vancomycin. For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertVanS-NarX
UseCell free expressionTagsMycExpressionBacterialMutationTransmembrane and sensing domain replaced to dete…PromoterT7Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBR322_ZA-ampR-PSEAB-2A
Plasmid#135230PurposeAAC6 experimental evolutionDepositorInsertaac6 Pseudomonas sp. ABAC61
UseTagsExpressionBacterialMutationPromoterpZAAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJPT002-M-HaloTag-1x FLAG
Plasmid#202493PurposeExpress "M" HaloTagDepositorInsertHaloTag
UseTags1x FLAG and Myristoylation siteExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJPT003-PM-HaloTag-1x FLAG
Plasmid#202494PurposeExpress "PM" HaloTagDepositorInsertHaloTag
UseTags1x FLAG and Palmitoylation-Myristoylation SiteExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJPT008-ZF6 M-synTF-1x FLAG
Plasmid#202498PurposeExpress "M" synTF (ZF6)DepositorInsertp65-ZF6
UseTags1x FLAG and Myristoylation siteExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJPT007-ZF6 synTF-1x FLAG
Plasmid#202497PurposeExpress "soluble" synTF (ZF6)DepositorInsertp65-ZF6
UseTags1x FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJPT006-1x FLAG-HaloTag-PPF
Plasmid#202496PurposeExpress "PPF" HaloTagDepositorInsertHaloTag
UseTags1x FLAG and Palmitoylation-Palmitoylation-Farnesy…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJPT010-ZF6 1x FLAG-synTF-G
Plasmid#202500PurposeExpress "G" synTF (ZF6)DepositorInsertp65-ZF6
UseTags1x FLAG and Geranylgeranyl siteExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJPT004-1x FLAG-HaloTag-G
Plasmid#202495PurposeExpress "G" HaloTagDepositorInsertHaloTag
UseTags1x FLAG and Geranylgeranyl siteExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSF-NgPglO
Plasmid#198204PurposepSN18 derivative encoding N. gonorrhoeae PglO with C-terminal FLAG epitope tagDepositorInsertNgPglO
UseTagsFlag tagExpressionBacterialMutationPromoterpBADAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
1056D
Plasmid#183132PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii bTub, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[bTub]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056E
Plasmid#183133PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii dsx, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[dsx]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056F
Plasmid#183134PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii tra, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[tra]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
BicC.Ca9.R
Plasmid#168295PurposeExpresses Cas9 in Drosophila late germline cellsDepositorInsertBicC.Cas9-T2A-eGFP-p10
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only