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Plasmid#125548PurposeGateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment2DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment2 attached to n-terminus of Gatew…ExpressionMammalian and YeastMutationPromotersynthetic promoter containing yeast, mammalian, a…Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only
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C098
Plasmid#174345PurposeTemplate for making Nluc mRNA reporter with pA139.DepositorInsertNanoLuc luciferase
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceSept. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
C071
Plasmid#174343PurposeTemplate for making Nluc mRNA reporter with pA29.DepositorInsertNanoLuc luciferase
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceSept. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
C093
Plasmid#174344PurposeTemplate for making Nluc mRNA reporter with pA63.DepositorInsertNanoLuc luciferase
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceSept. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:NLuc-I{AGO-405}:NosT
Plasmid#212182PurposeThis binary vector expresses Nanoluciferase (Nluc) containing the Argonaut (AGO) intron (405 bp into the gene) with the truncated 35sCAMV promoter and the tomato mosaic virus UTRDepositorInsertNanoLuc luciferase-I{AGO-405}
UseTagsExpressionPlantMutationAGO intron added at bp 405, eliminates expression…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEPYC0CM0133
Plasmid#154595PurposePhytobricks Level 0 partDepositorInsertLucN (NanoLuc)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBH111
Plasmid#215778PurposeNBU2 backbone with anti-LacI(YQR) and P(O1)_nanolucDepositorInsertsanti-LacI(YQR)
Nanoluc
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterBt1311 and P(O1)Available sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBH101
Plasmid#215772PurposeNBU2 backbone with LacI(YQR) and P(O1)_nanolucDepositorInsertsLacI(YQR)
Nanoluc
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterBT1311 and P(O1)Available sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBH102
Plasmid#215773PurposeNBU2 backbone with LacI(TAN) and P(tta)_nanolucDepositorInsertsLacI(TAN)
Nanoluc
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterBt1311 and P(tta)Available sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBH103
Plasmid#215774PurposeNBU2 backbone with LacI(KSL) and P(agg)_nanolucDepositorInsertsLacI(KSL)
Nanoluc
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterBt1311 and P(agg)Available sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBH104
Plasmid#215775PurposeNBU2 backbone with LacI(HQN) and P(ttg)_nanolucDepositorInsertsLacI(HQN)
Nanoluc
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterBt1311 and P(ttg)Available sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBH105
Plasmid#215776PurposeNBU2 backbone with LacI(GKR) and P(gac)_nanolucDepositorInsertsLacI(GKR)
Nanoluc
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterBt1311 and P(gac)Available sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBH106
Plasmid#215777PurposeNBU2 backbone with RbsR(YQR) and P(O1)_nanolucDepositorInsertsRbsR(YQR)
Nanoluc
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterBt1311 and P(O1)Available sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBH116
Plasmid#215779PurposeNBU2 backbone with anti-RbsR(YQR) and P(O1)_nanolucDepositorInsertsanti-RbsR(YQR)
Nanoluc
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterBt1311 and P(O1)Available sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-nLuc
Plasmid#199755PurposeControl construct of elongation reporter to quantify elongation of YFPDepositorInsertNanoluc
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPYC1CB0197
Plasmid#154654PurposeMoClo Level 1 luciferase reporterDepositorInsertNOS Nanoluc expression cassette [NOS::TMV::LucN::Flag::nosT]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPYCα2rKN0009
Plasmid#154616PurposeGoldenBraid level α luciferase reporterDepositorInsertMinSyn_066 Nanoluc expression cassette [MinSyn_066::LucN::Flag::nosT]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPYCα2rKN0010
Plasmid#154617PurposeGoldenBraid level α luciferase reporterDepositorInsertMinSyn_064 Nanoluc expression cassette [MinSyn_064::LucN::Flag::nosT]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPKKα2KN0091
Plasmid#154618PurposeGoldenBraid level α luciferase reporterDepositorInsertMinSyn_064 Nanoluc expression cassette [MinSyn_064::LucN::Flag::g7T]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPKKα2KN0092
Plasmid#154619PurposeGoldenBraid level α luciferase reporterDepositorInsertMinSyn_065 Nanoluc expression cassette [MinSyn_065::LucN::Flag::g7T]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only