Addgene

We narrowed to 790 results for: multisite gateway

Showing: 241 - 260 of 790 results

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pDest-302 (attR4-attR2)

Plasmid

#161894

Purpose

Multisite Gateway (attR4-attR2) vector for mammalian protein expression

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pDest-311 (attR4-attR3)

Plasmid

#161896

Purpose

Multisite Gateway (attR4-attR3) vector for mammalian protein expression

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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Ash1-5p

Plasmid

#169712

Purpose

Bacterial Expression of truncated yeast Ash1 including encoding amino acids 420-480 with phosphorylation sites 429 and 470 removed

Depositor

Tanja Mittag

Article

Martin et al J Am Chem Soc. 2016 Nov 30;138(47):15323

Insert

Ash1 (ASH1 Budding Yeast)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Truncation including encoding amino acids 420-480…

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli2 P1-6A

Plasmid

#51251

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848,923,956 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 789,805,817,848,923,956 mutated to Ala

Promoter

EF1a

Availability

Academic Institutions and Nonprofits only
pEF5/FRT/DEST-3xHA/mGli2 P1-4A

Plasmid

#51252

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 789,805,817,848 mutated to Ala

Promoter

EF1a

Availability

Academic Institutions and Nonprofits only
pEF5/FRT/DEST-3xHA/mGli2 P5-6A

Plasmid

#51253

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 923,956 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 923,956 mutated to Ala

Promoter

EF1a

Availability

Academic Institutions and Nonprofits only
pEF5/FRT/DEST-3xHA/mGli2 Pc-gE

Plasmid

#51256

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Glu

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 216,220,224,230,248 mutated to Glu

Promoter

EF1a

Availability

Academic Institutions and Nonprofits only
pEF5/FRT/DEST-3xHA/mGli2 Pc-gA/P1-6A

Plasmid

#51257

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248,789,805,817,848,923,956 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 216,220,224,230,248,789,805,817,848,9…

Promoter

EF1a

Availability

Academic Institutions and Nonprofits only
pME-mTagBFP

Plasmid

#75148

Purpose

TagBFP (blue) fluorophore for use with 5’ promoters.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

mTagBFP

Use

Middle entry vector for multisite gateway three f…

Tags

Expression

Mutation

Promoter

n/a

Availability

Academic Institutions and Nonprofits only

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pME-H2B-mCerulean3

Plasmid

#75156

Purpose

Nuclear mCerulean2 (blue) fluorophore for use with 5’ promoters.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

H2B-mCerulean3

Use

Middle entry vector for multisite gateway three f…

Tags

Expression

Mutation

Promoter

n/a

Availability

Academic Institutions and Nonprofits only

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p3E-MCS

Plasmid

#75174

Purpose

Multiple cloning site for insertion of gene of interest for C-terminal fusions.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

Multiple Cloning Site

Use

Three prime entry vector for multisite gateway th…

Tags

Expression

Mutation

Promoter

n/a

Availability

Academic Institutions and Nonprofits only

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pDONR P2R-P3-EYFP

Plasmid

#48351

Purpose

Contributes the coding sequence for EYFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

Depositor

Tomas Santalucia, Anna Planas

Article

Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

Insert

EYFP

Use

Gateway entry vector

Tags

Expression

Mutation

Contains a stop codon.

Promoter

none

Availability

Academic Institutions and Nonprofits only

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pDONR P2R-P3-ECFP

Plasmid

#48353

Purpose

Contributes the coding sequence for ECFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

Depositor

Tomas Santalucia, Anna Planas

Article

Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

Insert

ECFP

Use

Gateway entry vector

Tags

Expression

Mutation

Contains a stop codon.

Promoter

none

Availability

Academic Institutions and Nonprofits only

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pNJB80

Plasmid

#51518

Purpose

Gateway entry vector with attL5 and attL2 sites flanking a CmR and ccdB gene. In our hands, the multisite reaction still proceeds efficiently.

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Type

Empty backbone

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pENTR/D_creERt2

Plasmid

#27321

Depositor

Leonard Zon

Article

Mosimann et al Development. 2011 Jan . 138(1):169-77.

Insert

creERt2

Use

Multisite gateway middle entry vector

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pTORGm43GW

Plasmid

#112902

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin.

Depositor

Sebastian Schornack

Article

Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

Type

Empty backbone

Use

Oomycete expression

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest-623 (attR4-attR3)

Plasmid

#161878

Purpose

Multisite Gateway (attR4-attR3) vector for expression of multiple proteins using the Bac-to-Bac insect cell system

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest-624 (attR4-attR2)

Plasmid

#161879

Purpose

Multisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell system

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest-639 (attR4-attR2)

Plasmid

#161880

Purpose

Multisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell system

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest-676 (attR4-attR3)

Plasmid

#161888

Purpose

Multisite Gateway (attR4-attR3) vector for lentivirus production

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge