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Plasmid#67679PurposeMultisite gateway entry clone for adding C-terminal fusions of p3E-ML1N*2(7Q)DepositorInsertML1N*2(7Q)
UseGateway multisite 3' entry cloneTagsExpressionMutationPromoterAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
p3E-GBP
Plasmid#67672PurposeMultisite gateway entry clone for adding C-terminal fusions of GFP-binding nanobody (GBP)DepositorInsertanti-GFP nanobody
UseGateway multisite 3' entry cloneTagsExpressionMutationPromoterAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
p3E-TAPP1-PH
Plasmid#67675PurposeMultisite gateway entry clone for adding C-terminal fusions of TAPP1-PHDepositorInsertTAPP1-PH
UseGateway multisite 3' entry cloneTagsExpressionMutationPromoterAvailable sinceJune 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3E-OSBP
Plasmid#67677PurposeMultisite gateway entry clone for adding C-terminal fusions of OSBPDepositorInsertOSBP
UseGateway multisite 3' entry cloneTagsExpressionMutationaa 87-185 of NP_002547.1PromoterAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pME-2PH-PLCd
Plasmid#71283PurposeMultisite gateway entry clone for adding C-terminal fusions of human 2PH-PLC-deltaDepositorInsert2PH-PLC delta (Human)
UseGateway multisite middle entry cloneTagsExpressionMutationPromoterAvailable sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
p5E-EF1a (JDW 1164)
Plasmid#224473PurposeGateway compatible 5' entry clone containing the human EF1a promoterDepositorInsertEF1a promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
p5E-hs_ICAM_full (JDW 1047)
Plasmid#224479PurposeGateway compatible 5' entry clone containing the Human ICAM2 promoterDepositorInsertICAM2 promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-Sun1-2xsfGFP-6xMYC-pA (JDW 681)
Plasmid#224489PurposeGateway compatible middle entry clone containing Sun1 reporter fusion to 2 copies of sfGFP and 6 MYC tags (Nuclear envelope reporter and affinity tag)DepositorInsertSun1-2xsfGFP-6xMYC-pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME_Golgi-BFP_P2A_H2A_iRFP (JDW 1007)
Plasmid#224490PurposeGateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-iRFP (BFP golgi reporter and infrared RFP nuclear reporter)DepositorInsertGolgi-mTagBFP-P2A-H2A-iRFP
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME_Golgi-BFP_P2A_H2A_tdiRFP (JDW 1079)
Plasmid#224491PurposeGateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-tdiRFP (BFP golgi reporter and tandem infrared RFP nuclear reporter)DepositorInsertGolgi-mTagBFP-P2A-H2A-tdiRFP
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-rox-nls-mCherry-V5-2xStop-rox (JDW 1167)
Plasmid#224524PurposeA Gateway compatible middle entry clone containing a Rox flanked 3xNLS-mCherry-V5-2xStop cassetteDepositorInsertrox-3xNLS-mCherry-V5-2xSTOP-rox
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
p5E-EFS (EF1a core) (JDW 1319)
Plasmid#224474PurposeGateway compatible 5' entry clone with human alpha 2 globin pause site (to prevent transcription readthrough) and EF1a core promoter. Lacks downstream intron for a lower-activity, compact promoter.DepositorInsertEFS promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-LexA-mODC-LexOP-35SCaMV (JDW 1348)
Plasmid#224505PurposeGateway compatible middle entry clone containing a Destablized LexA and LexOperon with minimal CaMV 35S promoter as an all in one casette for RU486 driven gene expressionDepositorInsertLexA-mODC-LexOP-35SCaMV
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-LexA-mODC-LexOP-c-Fos (JDW 1359)
Plasmid#224506PurposeGateway compatible middle entry clone containing a Destablized LexA and LexOperon with 3' minimal c-Fos promoter for an all in one RU486 driven gene expression constructDepositorInsertLexA-oMDC-LexOP-c-Fos
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME TAEL2.0-5xC120-minpro (JDW 1109)
Plasmid#224507PurposeGateway compatible middle entry clone containing TAEL2.0 transcription factor and 5xC120 response element (All in one binary TAEL and effector domain clone)DepositorInsertTAEL2.0-5xC120
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-3xNLS-(G2SG2)2-Cre (JDW 1149)
Plasmid#224514PurposeA Gateway compatible middle entry clone containing a 3x nuclear localization signal followed by a multiple cloning site and a flexible linker fused to CreDepositorInsert3xNLS-(G2SG2)2-Cre
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox (JDW 1232)
Plasmid#224522PurposeA Gateway compatible middle entry clone containing loxP flanked 3xNLS-mCherry-V5-3xSTOP cassetteDepositorInsertlox-3xNLS-mCherry-V5-SV40pA-3xstop-lox
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-Actin-Vhh-sfGFP-P2A-1xHA-tdiRFP-caax (JDW 1311)
Plasmid#224497PurposeGateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and tdiRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)DepositorInsertActin-Vhh-sfGFP-P2A-HA-tdiRFP-caax
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-Actin-Vhh-sfGFP-P2A-1xHA-iRFP-caax (JDW 1216)
Plasmid#224496PurposeGateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and iRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)DepositorInsertActin-Vhh-sfGFP-P2A-HA-iRFP-caax
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-YAP1
Plasmid#79503PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human YAP1 CDSDepositorInsertYAP1 (YAP1 Human)
UseMultisite gateway entry cloneTagsFLAGExpressionMutationPromoterno PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only