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pcDNA3-Flag OSM

Plasmid

#12191

Depositor

Gary Johnson

Article

Uhlik et al Nat Cell Biol. 2003 Dec . 5(12):1104-10.

Insert

OSM (Ccm2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only
pSG5 EDD

Plasmid

#37191

Depositor

Charles Watts, Darren Saunders

Article

Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

Insert

EDD (UBR5 Human)

Use

Tags

6x His Tag and FLAG

Expression

Mammalian

Mutation

K503R (please see depositor comments below)

Promoter

SV40

Available since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[S199A][S227A]-FLAG

Plasmid

#199323

Purpose

double mutated phosphorylation site (kinase acitivity and MLKL interaction inhibited)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 199 and 227 to Alanine

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-TDAG51

Plasmid

#32699

Depositor

Richard C. Austin

Article

Hossain et al J Biol Chem. 2003 Aug 8;278(32):30317-27

Insert

human tdag51 (PHLDA1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only
CFP-OSM

Plasmid

#12190

Depositor

Gary Johnson

Article

Uhlik et al Nat Cell Biol. 2003 Dec . 5(12):1104-10.

Insert

OSM (Ccm2 Mouse)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA1-GATA4-opposite orientation

Plasmid

#51928

Purpose

for T7 directed antisense production of mouse GATA4

Depositor

Jeffery Molkentin

Article

Liang et al J Biol Chem. 2001 Aug 10;276(32):30245-5

Insert

GATA4 reverse orientation (Gata4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 28, 2014

Availability

Academic Institutions and Nonprofits only
pCDB181

Plasmid

#80692

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEHEE_06

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gEHEE_06

Use

Tags

OsmY-10His-Smt3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCDB267

Plasmid

#80695

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gHHH_06

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gHHH_06

Use

Tags

OsmY-10His-Smt3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDB278

Plasmid

#80694

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gHH_44

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gHH_44

Use

Tags

OsmY-10His-Smt3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCDB294

Plasmid

#80693

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gHEEE_02

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gHEEE_02

Use

Tags

OsmY-10His-Smt3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCDB199

Plasmid

#80691

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEHE_06

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gEHE_06

Use

Tags

OsmY-10His-Smt3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDB289

Plasmid

#80690

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEEHE_02

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gEEHE_02

Use

Tags

OsmY-10His-Smt3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDB185

Plasmid

#80688

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEEH_04

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gEEH_04

Use

Tags

OsmY-10His-Smt3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDB285

Plasmid

#80687

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEEEH_04

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gEEEH_04

Use

Tags

OsmY-10His-Smt3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-Cerulean-UCR1

Plasmid

#67578

Purpose

Expresses the UCR1 region of PDE4D3 in mammalian cells

Depositor

Graeme Carnegie

Article

Wang et al Cell Signal. 2015 May;27(5):908-22. doi:

Insert

Upstream conserved region 1 of phosphodiesterase 4D3 (PDE4D Human)

Use

Tags

Cerulean and FLAG

Expression

Mammalian

Mutation

Amino acid residues 17-136 of PDE4D3

Promoter

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-RIPK3[V458A][Q459A][V460A][G461A]-FLAG

Plasmid

#199328

Purpose

mutated RHIM motive (interaction inhibited)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed V458, Q459,V460 and G461 to alanine

Promoter

Available since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[S199D]-FLAG

Plasmid

#199315

Purpose

mutated phosphorylation site (kinase acitivity permanent active)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 199 to Aspartic Acid

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-RIPK3[S199A]-FLAG

Plasmid

#199313

Purpose

mutated phosphorylation site (kinase acitivity inhibited)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 199 to Alanine

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRANS-Pho2-1/2-2-Csy4

Plasmid

#161763

Purpose

pTRANS T-DNA vector with nptII selection and 35S:Cas9 with 6x Csy4-spliced gRNAs and rolD:TREX2 targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pTRANS_220 - #91113)

Depositor

Shaun Curtin

Article

Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6

Insert

Cas9 and gRNAs

Use

Tags

Expression

Plant

Mutation

Promoter

CmYLCV Promoter

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRANS-Pho2-1/2-2-tRNA

Plasmid

#161762

Purpose

pTRANS T-DNA vector with nptII selection and 35S:Cas9 with 6x tRNA-spliced gRNAs and rolD:TREX2 targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pTRANS_220 - #91113)

Depositor

Shaun Curtin

Article

Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6

Insert

Cas9 and gRNAs

Use

Tags

Expression

Plant

Mutation

Promoter

CmYLCV Promoter

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge