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Showing: 241 - 260 of 650 results

  1. pcDNA3.1-nAChr-Alpha6-GFP

    Plasmid
    #50486
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Use
    Tags
    GFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the GFP open…
    Promoter
    CMV
    Available since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  3. pILGFP9E3

    Plasmid
    #185842
    Purpose
    Testing the TEF1 promoter inserted with 4 TetO elements using a green fluorescence protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1-PTEF1+[TetO]>yEGFP>TPGK1-TURA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    185842-plasmid-map-sequence-id-368107

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  4. pILGFP4D42

    Plasmid
    #185840
    Purpose
    Testing the TEF1 promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1-PTEF1+[Z268]>yEGFP>TPGK1-TURA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    185840-plasmid-map-sequence-id-369622

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  5. pILGFP10CF71

    Plasmid
    #185860
    Purpose
    Testing the SkGAL2 promoter inserted with 4 Z268 elements using a green fluorescent protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1- PSkGAL2+[Z268]>(BamHI)yEGFP>TURA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185860-plasmid-map-sequence-id-370799

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  9. pILGFP3A2 (2)

    Plasmid
    #185852
    Purpose
    Testing the TEF1 promoter appended with 3 tetracycline riboswitch using a green fluorescence protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1-PTEF1>3*TcRb>yEGFP>TPGK1-TURA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185852-plasmid-map-sequence-id-370761

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  10. pILGFP4D41

    Plasmid
    #185841
    Purpose
    Testing the CYC1 core promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1-PCYC1+[Z268]>yEGFP>TPGK1-TURA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    185841-plasmid-map-sequence-id-369628

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  12. pILGFP10CF72

    Plasmid
    #185857
    Purpose
    Testing the SkGAL2 promoter inserted with 4 PZ4 elements using a green fluorescent protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1- PSkGAL2M4(4*PZ4)>(BamHI)yEGFP>TURA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185857-plasmid-map-sequence-id-370796

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  13. pILGFP10E1B

    Plasmid
    #185856
    Purpose
    Testing the SkGAL2 promoter inserted with 3 PZ4 elements using a green fluorescent protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1- PSkGAL2M2(3*PZ4)>(BamHI)yEGFP>TURA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185856-plasmid-map-sequence-id-370794

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  14. pILGFP10E1A

    Plasmid
    #185855
    Purpose
    Testing the SkGAL2 promoter inserted with 2 PZ4 elements using a green fluorescent protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1- PSkGAL2M1(2*PZ4)> (BamHI)yEGFP>TURA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185855-plasmid-map-sequence-id-370793

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  15. pILGFP8EFA

    Plasmid
    #185854
    Purpose
    Testing a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1-PTEF1>UBI4>RHGSGTMV>DHFRP66L>yEGFP>TPGK1-TURA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    DHFR P66L
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185854-plasmid-map-sequence-id-370766

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  16. pILGFP3A2 (1)

    Plasmid
    #185851
    Purpose
    Testing the TEF1 promoter appended with 2 tetracycline riboswitch using a green fluorescence protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1-PTEF1>2*TcRb>yEGFP>TPGK1-TURA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185851-plasmid-map-sequence-id-370760

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  19. pLTR.gp140/EGFP.Revdelta38/DsRed

    Plasmid
    #115775
    Purpose
    HIV splicing reporter allows the assessment of the effect of LRAs on HIV-1 transcription and splicing.
    Depositor
    Damian Purcell
    Article
    Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37
    Insert
    Envelope fused to EGFP (gp140-EGFP) and non-functional Rev protein fused to DsRed fluorescent protein (Rev-delta38-DsRed).
    Use
    Tags
    EGFP; dsReD
    Expression
    Mammalian
    Mutation
    gp140 uncleaved (1190-91aa, KR > TG; truncated…
    Promoter
    HIV-1 (LTR)
    Available since
    Oct. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    115775-plasmid-map-sequence-id-224438

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Showing: 241 - 260 of 650 results