-
Plasmid#64386Purposeencodes c8 gRNADepositorInsertc8 gRNA
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRPR1_c6gRNA_RPR1t
Plasmid#64384Purposeencodes c6 gRNADepositorInsertc6 gRNA
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDest_U6_A2_CG2_T1snp_gRNA
Plasmid#213789PurposepDEL114; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type I (SNP) sgRNADepositorInsertnrg1 type I SNP sgRNA (nrg1 Zebrafish)
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRPR1_c2gRNA_RPR1t
Plasmid#64380Purposeencodes c2 gRNADepositorInsertc2 gRNA
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRPR1_g1gRNA_RPR1t
Plasmid#64387Purposeencodes g1 gRNADepositorInsertg1 gRNA
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRPR1_c7gRNA_RPR1t
Plasmid#64385Purposeencodes c7 gRNADepositorInsertc7 gRNA
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRPR1_c1gRNA_RPR1t
Plasmid#64379Purposeencodes c1 gRNADepositorInsertc1 gRNA
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRPR1_c5gRNA_RPR1t
Plasmid#64383Purposeencodes c5 gRNADepositorInsertc5 gRNA
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCFD3.1-w-dU6:3gRNA
Plasmid#123366PurposegRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).DepositorInsertU6:3-gRNA
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
IL1RN gRNA3
Plasmid#113132PurposeExpresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)DepositorInsertIL1RN guideRNA 3 (SpCas9 scaffold), co-expressed GFP (transfection marker)
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPlacPbuCas13b-gRNA-NT
Plasmid#184838PurposeExpression of single-spacer CRISPR array with a shortened non-targeting spacer for PbuCas13b and expression of PbuCas13b in bacteria.DepositorInsertCo-expression of single-spacer CRISPR array with a nontargeting spacer for PbuCas13b and PbuCas13b nuclease in bacteria.
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119 and lac promoterAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
IL1RN gRNA2
Plasmid#113131PurposeExpresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)DepositorInsertIL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
sgRNA with U6 promoter
Plasmid#48962Purposeto drive the sgRNA expression under a U6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionWormMutationPromoterU6Available sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC19-gRNA
Plasmid#137776PurposeContains the T7 promoter and gRNA scaffold and is used for the in vitro transcription of gRNAsDepositorInsertgRNA scaffold
UseCRISPRTagsExpressionMutationpUC19 BsaI destroyedPromoterT7Available sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSPneoHHgRNAH
Plasmid#63557PurposeExpresses gRNA in LeishmaniaDepositorInsertguide sequence
UseLeishmania expressionTagsExpressionMutationPromoterAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSPneogRNAH
Plasmid#63556PurposeExpresses gRNA in LeishmaniaDepositorInsertguide sequence
UseLeishmania expressionTagsExpressionMutationPromoterAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pgRNA_AT
Plasmid#196243PurposeBackbone plasmid for cloning sgRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterJ23119Available sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRPR1_g2gRNA_RPR1t
Plasmid#64388Purposeencodes g2 gRNADepositorInsertg2 gRNA
UseTagsExpressionYeastMutationPromoterpRPR1Available sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC-H1-gRNA
Plasmid#61089PurposeCRISPR/Cas9 system gRNA cloningDepositorInsertH1 promoter; gRNA sequences
UseCRISPR; /cas9 grna cloning vectorTagsExpressionMammalianMutationPromoterH1Available sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHelper_ShCAST_sgRNA
Plasmid#127921PurposeExpresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).DepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
UseTagsExpressionMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only