Addgene

We narrowed to 433 results for: TagRFP

Showing: 241 - 260 of 433 results

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pLenti-tetON-NKX2-1-I207F -EF1a-TagRFP-2A-tet3G

Plasmid

#204212

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (I207F) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NK2 homeobox 1 (NKX2-1 Human)

Use

Lentiviral

Tags

Point mutation on I207F

Expression

Mammalian

Mutation

Promoter

TetON

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1-I207M -EF1a-TagRFP-2A-tet3G

Plasmid

#204213

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (I207M) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Point mutation on I207M

Promoter

TetON

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1(delDBD)-EF1a-TagRFP-2A-tet3G

Plasmid

#204214

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (delDBD) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Deletion of DNA binding domain (delDBD)

Promoter

TetON

Availability

Academic Institutions and Nonprofits only

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AAV-puro-BGHpA-TRE-pEF-tagRFP-T-NLS

Plasmid

#163083

Purpose

Donor vector for integration of TagRFP-T reporter at AAVS1 safe harbor site via TALENs

Depositor

Lacra Bintu

Article

Van et al Nat Commun. 2021 Jan 22;12(1):537. doi:

Insert

TagRFP-T

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Availability

Academic Institutions and Nonprofits only

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pSLPB2-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

Plasmid

#187945

Purpose

dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Insert

SpdCas9-tagRFPt-P2A-tagBFP

Use

Synthetic Biology; Piggybac

Tags

NLS, NLS + HA-tag, P2A, tagBFP, and tagRFPt

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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Tol2-pA-GI-TagRFP-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP

Plasmid

#135812

Purpose

This plasmid can be used to express independently eGFP and TagRFP from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.

Depositor

Nadia Mercader Huber

Article

Sanz-Morejon et al Cell Rep. 2019 Jul 30;28(5):1296-1306.e6

Insert

TagRFP

Use

Zebrafish

Tags

Expression

Mutation

Promoter

UAS

Availability

Academic Institutions and Nonprofits only

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pCFJ2240 - [1-2] ENTR - Fluor - ce-tagRFP-T (1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159855

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-tagRFP-T (1 intron, syntrons(3), no_atg, no_stop)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G

Plasmid

#167935

Purpose

Inducible CRISPRi expression

Depositor

Emma Rawlins

Article

Sun et al Elife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

KRAB-dCas9-DHFR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

tetON

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-dxCas9(3.7)-VPR-EF1a-TagRFP-2A-tet3G

Plasmid

#167937

Purpose

Inducible CRISPRa expression

Depositor

Emma Rawlins

Article

Sun et al Elife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

dxCas9(3.7)-VPR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

tetON

Availability

Academic Institutions and Nonprofits only

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pSLPB2B-ER50-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

Plasmid

#187948

Purpose

ER50 degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Insert

ER50-SpdCas9-tagRFPt-P2A-tagBFP

Use

CRISPR and Synthetic Biology

Tags

GGS linker

Expression

Mammalian

Mutation

6 mutations, T371A, L384M, M421G, G521R, Y537S, N…

Promoter

Availability

Academic Institutions and Nonprofits only

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pSLPB2B-SMASh-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

Plasmid

#187949

Purpose

SMASh degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Insert

SMASh-SpdCas9-tagRFPt-P2A-tagBFP

Use

CRISPR and Synthetic Biology

Tags

GGS linker

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pSLPB2B-ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

Plasmid

#187950

Purpose

ecDHFR degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Insert

ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP

Use

CRISPR and Synthetic Biology

Tags

GGS linker

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pSLPB2B-FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

Plasmid

#187951

Purpose

FKBP12 (F36V mutant) degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Insert

FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP

Use

CRISPR and Synthetic Biology

Tags

GGS linker

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ2307 - [1-2] ENTR - Fluor - ce-tagRFP-T(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

Plasmid

#159856

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-tagRFP-T(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ2437 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159857

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ2442 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159858

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Availability

Academic Institutions and Nonprofits only

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pK098.CAG-loxP-stop-loxP-nlstagRFP-ires-tTA-WPRE (Supernova)

Plasmid

#85179

Purpose

For nucleus-specific RFP labeling in single cells, pK098 should be used with pK031.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

nuclear localization signal (nls) tagRFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pMDJ22 - pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Plasmid

#159814

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Availability

Academic Institutions and Nonprofits only

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pMDJ23 - pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Plasmid

#159815

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Availability

Academic Institutions and Nonprofits only

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pSLPB3B-AID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1

Plasmid

#187960

Purpose

AID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Insert

AID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1

Use

CRISPR and Synthetic Biology

Tags

GGS linker

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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