Addgene

We narrowed to 10,607 results for: SHA;

Showing: 241 - 260 of 10607 results

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pAS1-Prig-3::tagBFP

Plasmid

#165598

Purpose

rig-3 promoter derive BFP expression in AVA head neurons.

Depositor

Andrew Leifer

Article

Hallinen et al Elife. 2021 Jul 29;10:e66135. doi: 10.75

Insert

Prig-3, tagBFP

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRS267

Plasmid

#122380

Purpose

CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -112 bp upstream of HSP90 start codon

Depositor

Rebecca Shapiro

Article

Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

Insert

HSP90

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pQP-2473

Plasmid

#138976

Purpose

Nucleus-to-cytoplasm relocalization of genomically expressed GFP-fusion using NIR light controlled intrabody.

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-BphP1-NES-T2A- iB(GFP)-NES-mCherry-QPAS1- NLS-NotI

Use

Tags

Expression

Mutation

Promoter

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pQP-CiRISB

Plasmid

#138978

Purpose

Light-controlled tri-directional targeting of endogenous proteins (cytoplasm-shifted)

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-BphP1-CAAX-IRES2-iB(GFP)-NESmCherry- Q-PAS1-AsLOV2cNLS-NotI

Use

Tags

Expression

Mutation

Promoter

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pQP-AR10

Plasmid

#138980

Purpose

Endogenous RAS regulation using light-controlled intrabody

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

BglII-BphP1-NES-T2A- iB(RAS)-NES-mCherry-QPAS1- NLS-NotI

Use

Tags

Expression

Mutation

Promoter

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pQP-NiRISB

Plasmid

#138977

Purpose

Light-controlled tri-directional targeting of endogenous proteins (nucleus-shifted)

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-BphP1-CAAX-IRES2-NES- iB(GFP)- mCherry-Q-PAS1-AsLOV2cNLS-NotI

Use

Tags

Expression

Mutation

Promoter

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pQP-iRISB

Plasmid

#138979

Purpose

Light-controlled tri-directional targeting of endogenous proteins

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-BphP1-CAAX-IRES2-NES-SGlinkeriB( GFP)-mCherry-Q-PAS1-AsLOV2cNLS-NotI

Use

Tags

Expression

Mutation

Promoter

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pQP-2363

Plasmid

#138975

Purpose

Relocalization of genomically expressed protein of interest from cytoplasm to nucleus upon near-infrared illumination

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-BphP1- iB(GFP) -IRES2-NES-mCherry-QPAS1- NLS-NotI

Use

Tags

Expression

Mutation

Promoter

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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Mouse lipid metabolism knockout library

Pooled Library

#206248

Purpose

The lipid metabolism library contains guide RNAs against 295 genes targeting mouse genes encoding enzymes that directly impact lipid metabolism.

Depositor

Kivanc Birsoy

Article

Soula et al Nature. 2024 Aug 7. doi: 10.1038/s41586-

Use

CRISPR and Lentiviral

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only
lentiGuide-Puro-pKO-library

Pooled Library

#190744

Purpose

CRISPR/Cas9 paired-guide RNA library to delete proximal poly(A) sites in mouse melanoma.

Depositor

Robert Bradley

Article

Gabel et al Nat Commun. 2024 Feb 1;15(1):959. doi: 1

Expression

Mammalian

Species

Mus musculus

Use

CRISPR, Lentiviral, and Mouse Targeting

Available since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only
EFS-mTSG

Pooled Library

#113584

Purpose

AAV-CRISPR library for pool mutagenesis of top tumor suppressor genes

Depositor

Sidi Chen

Article

Wang et al Sci Adv. 2018 Feb 28;4(2):eaao5508. doi:

Expression

Mammalian

Use

AAV, CRISPR, Lentiviral, and Luciferase

Available since

July 23, 2019

Availability

Academic Institutions and Nonprofits only
TBG-mTSG

Pooled Library

#113585

Purpose

AAV-CRISPR library for pool mutagenesis of top tumor suppressor genes in mouse liver

Depositor

Sidi Chen

Article

Wang et al Sci Adv. 2018 Feb 28;4(2):eaao5508. doi:

Expression

Mammalian

Use

AAV, CRISPR, Lentiviral, and Luciferase

Available since

July 23, 2019

Availability

Academic Institutions and Nonprofits only
pRS156

Plasmid

#182707

Purpose

CRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning site)

Depositor

Rebecca Shapiro

Article

Gervais et al G3 (Bethesda). 2022 Dec 1:jkac301. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-V5-DGCR8

Plasmid

#51383

Depositor

Narry Kim

Article

Yeom et al Nucleic Acids Res. 2006;34(16):4622-9. E

Insert

DGCR8 (DGCR8 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO human ULK1 shRNA 8

Plasmid

#27633

Depositor

Reuben Shaw

Article

Egan et al Science. 2010 Dec 23. ():.

Insert

human ULK1 shRNA 8

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 16, 2011

Availability

Academic Institutions and Nonprofits only

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pLKO human ULK2 shRNA 91

Plasmid

#27634

Depositor

Reuben Shaw

Article

Egan et al Science. 2010 Dec 23. ():.

Insert

human ULK2 shRNA 91

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2011

Availability

Academic Institutions and Nonprofits only
pLKO mouse ULK2 shRNA 93

Plasmid

#27635

Depositor

Reuben Shaw

Article

Egan et al Science. 2010 Dec 23. ():.

Insert

mouse ULK2 shRNA 93

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2011

Availability

Academic Institutions and Nonprofits only
pQP-2460

Plasmid

#138973

Purpose

Light-induced recruitment of QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NES-mCherry-Q-PAS1-NotI

Use

Tags

Expression

Mutation

Promoter

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pRSETC-PITPbeta (splice variant 1)

Plasmid

#58270

Purpose

bacterial expression of PITP beta (phosphatidylinositol transfer protein beta)

Depositor

Shamshad Cockcroft

Article

Shadan et al Traffic. 2008 Sep;9(10):1743-56. doi: 10

Insert

PITP beta (PITPNB Human)

Use

Tags

HIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
NmMetNI-WT

Plasmid

#172114

Purpose

Expression of MetN and MetI membrane proteins

Depositor

Douglas Rees

Article

Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:

Inserts

MetN
MetI

Use

Tags

10x His Tag and Enterokinase

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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