Addgene

We narrowed to 1,052 results for: Anti-CRISPR

Showing: 241 - 260 of 1052 results

Results per page:

Sort results by:

pVAX_CBI_AcrIIA6_D1811

Plasmid

#136667

Purpose

Expresses human codon-optimized AcrIIA6 D1811 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

Depositor

Yannick Doyon

Article

Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

Insert

AcrIIA6_D1811

Use

Synthetic Biology

Tags

No tag, No NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pVAX_CBI_AcrIIA6_D1024

Plasmid

#136666

Purpose

Expresses human codon-optimized AcrIIA6 D1024 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

Depositor

Yannick Doyon

Article

Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

Insert

AcrIIA6_D1024

Use

Synthetic Biology

Tags

No tag, No NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pVAX_CBI_AcrIIA5_D4276

Plasmid

#136664

Purpose

Expresses human codon-optimized AcrIIA5 D4276 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

Depositor

Yannick Doyon

Article

Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

Insert

AcrIIA5_D4276

Use

Synthetic Biology

Tags

No tag, No NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pVAX_CBI_AcrIIA5_D1126

Plasmid

#136663

Purpose

Expresses human codon-optimized AcrIIA5 D1126 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

Depositor

Yannick Doyon

Article

Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

Insert

AcrIIA5_D1126

Use

Synthetic Biology

Tags

No tag, No NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pKEW270-pTet-empty

Plasmid

#115669

Purpose

Vector for anti-CRISPR expression with pTet

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcloDF-kan

Plasmid

#184837

Purpose

Backbone that confers Kanamycin resistance, contains cloDF ori

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcloDF-amp

Plasmid

#184844

Purpose

Backbone that confers Ampicillin resistance, contains cloDF ori

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ7751 pHR Ef1α: mCherry-P2A-AcrIIA4

Plasmid

#125148

Purpose

Lentiviral vector for constitutive expression of AcrIIA4 with mCherry fluorescent reporter.

Depositor

Stanley Qi

Article

Nakamura et al Nat Commun. 2019 Jan 14;10(1):194. doi:

Insert

AcrIIA4-P2A-mCherry

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-hAcrVA2.1-NLS(sv40) (BPK5059)

Plasmid

#115137

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA2.1 anti-CRISPR protein with C-terminal NLS

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA2.1 anti-CRISPR protein

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-hAcrVA1-NLS(sv40) (BPK5050)

Plasmid

#115136

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA1 anti-CRISPR protein with C-terminal NLS

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA1 anti-CRISPR protein

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ7780 mU6:- || CMV: mCherry~P2A-DD-AcrIIA4

Plasmid

#123658

Purpose

DD-AcrIIA4 fusion for Shield1-mediated AcrIIA4 control without sgRNA following mU6 promoter.

Depositor

Stanley Qi

Article

Nakamura et al Nat Commun. 2019 Jan 14;10(1):194. doi:

Type

Empty backbone

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-hAcrVA3.1-NLS(sv40) (RTW2624)

Plasmid

#115139

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA3.1 anti-CRISPR protein with C-terminal NLS

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA3.1 anti-CRISPR protein

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-hAcrVA3-NLS(sv40) (BPK5077)

Plasmid

#115140

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA3 anti-CRISPR protein with C-terminal NLS

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA3 anti-CRISPR protein

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-hAcrVA2-NLS(sv40) (AAS2283)

Plasmid

#115138

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA2 anti-CRISPR protein with C-terminal NLS

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA2 anti-CRISPR protein

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CNC3G (CASANOVA-C3 with glycine linkers)

Plasmid

#137192

Purpose

Expresses CNC3G (CASANOVA-C3 with glycine linkers), a blue light dependent NmeCas9 inhibitory protein

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2021 Mar 18;49(5):e29

Insert

CNC3G (CASANOVA-C3 with glycine linkers)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-hOrf2mor-NLS(sv40) (BPK5095)

Plasmid

#115141

Purpose

CMV-T7 promoter expression plasmid for human codon optimized Orf2mor anti-CRISPR protein with C-terminal NLS

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized Orf2mor anti-CRISPR protein

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH376

Plasmid

#86842

Purpose

mamalian expression of anti-CRISPR protein AcrIIA4

Depositor

Joseph Bondy-Denomy

Article

Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

Insert

acrIIA4

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH373

Plasmid

#86840

Purpose

mamalian expression of anti-CRISPR protein AcrIIA2

Depositor

Joseph Bondy-Denomy

Article

Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

Insert

acrIIA2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pPJ23116-MS2-rep

Plasmid

#184842

Purpose

Expression of the rep protein of phage MS2

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

Replicase gene MS2 phage

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23116

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
P70a-deGFP-sc101-kan

Plasmid

#184841

Purpose

Expression of deGFP

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

green fluorescent protein

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

promoter OR2-OR1

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge