Addgene

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KNtp-SUR1 core fusion

Plasmid

#216212

Purpose

for expression of KNtp-SUR1 fusion protein in BacMam system

Depositor

Lei Chen

Article

Wang et al Front Pharmacol. 2022 Jun 30;13:929684.

Insert

SUR1 core

Use

Tags

GFP-MBP

Expression

Mammalian

Mutation

deletion of TMD0

Promoter

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor_Tn[GFP]

Plasmid

#213903

Purpose

Mariner transposon expressing mNeonGreen with kan selection casette

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with kan resistance and mNeonGreen

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor_Tn[RFP]

Plasmid

#213904

Purpose

Mariner transposon expressing mCherry with cam selection casette

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with cam resistance and mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVK_AE(J23106-RiboJ-BCD2-E0040m-B0015)

Plasmid

#197673

Purpose

Expresses GFP under the control of J23106 and RiboJ-BCD2

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVK_AE(J23106-RiboJ-UTR1-E0040m-B0015)

Plasmid

#197674

Purpose

Expresses GFP under the control of J23106 and RiboJ-UTR1

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVK_AE(J23100-RiboJ-BCD2-E0040m-B0015)

Plasmid

#197661

Purpose

Expresses GFP under the control of J23100 and RiboJ-BCD2

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVK_AE(J23103-RiboJ-BCD2-E0040m-B0015)

Plasmid

#197667

Purpose

Expresses GFP under the control of J23103 and RiboJ-BCD2

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVK_AE(J23103-RiboJ-UTR1-E0040m-B0015)

Plasmid

#197668

Purpose

Expresses GFP under the control of J23103 and RiboJ-UTR1

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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ISA431: aTc-MP6

Plasmid

#189935

Purpose

Mutagenesis Plasmid: pTet, danQ926, dam, seqA, emrR, ugi and cda1, tetR, CloDF13, KanR

Depositor

Nathan Crook

Article

Al'Abri et al Nucleic Acids Res. 2022 Jun 10;50(10):e5

Insert

danQ926, dam, seqA, emrR, ugi and cda1

Use

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pSelect-7

Plasmid

#190244

Purpose

Encodes lacI- and pheA-targeting gRNAs for positive and negative selections of dCas9 activity.

Depositor

Marc Ostermeier

Article

Spisak et al PLoS One. 2022 Jun 3;17(6):e0269270. doi

Insert

LacI

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pSelect-9

Plasmid

#190245

Purpose

Encodes lacI- and aadA-targeting gRNAs for positive and negative selections of dCas9 activity.

Depositor

Marc Ostermeier

Article

Spisak et al PLoS One. 2022 Jun 3;17(6):e0269270. doi

Insert

LacI

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pJH572

Plasmid

#172156

Purpose

Negative control pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCAFNF-EGFP

Plasmid

#163609

Purpose

Expresses EGFP Flp-dependently in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Egfp

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pFVTd5

Plasmid

#167520

Purpose

Broad-Host range expression vector, RSF1010 origin, VanRAM repressor, PVanCC promoter, tdKatushka2, Gentamicin Resistance Marker

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

VanRAM-tdKatushka2

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PVanCC

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pVG10

Plasmid

#164702

Purpose

yEVenus under the control of GALL promoter

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

Insert

GALLpr - yEVenus - CLN2 PEST - ADH1t

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pVG50

Plasmid

#164708

Purpose

yEVenus under the control of tetOpr with ADH1t upstream of the reporter

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

Insert

ADH1t - tetOpr - yEVenus - CLN2 PEST - ADH1t

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pVG88

Plasmid

#164709

Purpose

yEVenus under the control of ARG3 promoter

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

Insert

ARG3pr - yEVenus - CLN2 PEST - ADH1t

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pFTtG5

Plasmid

#149488

Purpose

Broad-Host range expression vector, RSF1010 origin, TtgR(AM) repressor, PTtg promoter, mRFP.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

TtgR(AM)-mRFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PTtg

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pFVGe2

Plasmid

#149500

Purpose

Broad-Host range expression vector, RSF1010 origin, VanR(AM) repressor, PVanCC promoter, GmR, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

VanR(AM)-GmR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PVanCC

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBLlCa5

Plasmid

#149501

Purpose

Broad-Host range expression vector, pBBR origin, LacI repressor, PLlacO-1 promoter, AmpR, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LacI-AmpR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PLlacO-1

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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